Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,669,271 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,669,2710GC99.7% 693.4 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (139/153);  total (140/153)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01
*CP0425831,669,2720CG99.7% 692.6 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (139/153);  total (140/153)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,669,2730TA99.3% 691.4 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (138/153);  minor base G (2/0);  total (140/153)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

CACCAGCACGATGCGTTCACGGTGCTGCGGCAGAAAATGTTTGCCGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGGCTGTTCTTTTTGCTTACGCCAGCCAGGCTAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATGCTTTCAAAACCGCGACGGAT  >  CP042583/1668995‑1669546
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                  
caccaGCACGATGCGTTCACGGTGCTGCGGCAGAAAATGTTTGCCGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTAGCGCAGGCAAAACCGTTCGCCCGCCCGAGCGGGATCttttt                                                                                                                                                                                                                                                                           >  2:1419607/1‑288 (MQ=255)
           tGCGTTCACGGTGCTGCGGCAGAAAATGTTTGCCGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACgccag                                                                                                                                                                                                                                                                >  1:2150835/1‑288 (MQ=255)
                   cGGTGCTGCGGCAGAAAATGTTTGCCGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCTCCGGA‑CGACGCGCGTCGATAATGCGTACCCC‑ATCAAAAAACAGCGTGCCCTGGGTCTCGCAGGCAAAACCTTGCGCCCGCCCGAGCGAGTTCTTTCTCGATACGccagccagcgaca                                                                                                                                                                                                                                                       >  2:2506008/1‑284 (MQ=255)
                                gAAAATGTTTGCCGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAgg                                                                                                                                                                                                                                         >  1:25916/1‑289 (MQ=255)
                                    aTGTTTGCCGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGaaa                                                                                                                                                                                                                                     <  1:1115391/289‑1 (MQ=255)
                                         tgcctccGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCTTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGc                                                                                                                                                                                                                                <  1:503336/283‑1 (MQ=255)
                                         tcccGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGc                                                                                                                                                                                                                                <  1:1841860/287‑1 (MQ=255)
                                               cGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCGGGTTTTT‑ACGGTTT‑CGAGCCACAACAACGCCCGACCGCCCCCCTCCGTTAAT‑CGTAACACAACCAAAAAAAACCGTCCCCTGGTTA‑CGCCAGCAAAACCCGTCCCCCGCCCCAGCCGGGTCCTTTTTCATACCGCAACCCGGCAAAAAGGCCACACAGGGAAAACCGCCCAGaaa                                                                                                                                                                                                                          >  2:2191176/1‑289 (MQ=255)
                                                gatgatTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTaaa                                                                                                                                                                                                                         <  1:2354019/289‑1 (MQ=255)
                                                gatgatTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGAGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGCATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCAAAAAGGG‑CTGACAAGGAAAACCggaacataaa                                                                                                                                                                                                                         >  2:2678238/1‑281 (MQ=255)
                                                     ttttCGGCTCGTCTGGCCCATTATCTTCTGCATCAGCCCCGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATc                                                                                                                                                                                                                    <  1:309636/289‑1 (MQ=255)
                                                     ttttCGACTCGTCTGGCCCATTATCTTCTGCATCCGCCCCATCATAGCCCGGTTCGTCCAGCGTCTGCATGATGATGCGGAACATTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGGGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATc                                                                                                                                                                                                                    <  2:1102960/289‑1 (MQ=255)
                                                          ggATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGtt                                                                                                                                                                                                               <  2:416653/289‑1 (MQ=255)
                                                               gTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCg                                                                                                                                                                                                                   >  1:763681/1‑280 (MQ=255)
                                                               gTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCg                                                                                                                                                                                                                   <  2:763681/280‑1 (MQ=255)
                                                                tCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑ATGCCAAGGGAAACCGGCCAGTAAAACATCTGGTTCAGGaa                                                                                                                                                                                                         >  2:1286498/1‑289 (MQ=255)
                                                                    gCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAAtgtg                                                                                                                                                                                                     >  1:851974/1‑289 (MQ=255)
                                                                     cccATTCTCTTCTGCATCAGCCACGTCAGAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAAtgtg                                                                                                                                                                                                     <  1:581814/288‑1 (MQ=255)
                                                                      ccATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATgtgt                                                                                                                                                                                                    <  1:1615081/288‑1 (MQ=255)
                                                                         ttATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGAc                                                                                                                                                                                                >  1:634885/1‑289 (MQ=255)
                                                                          tATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑AGACGCGCGTCGATAATGCGTACCAA‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCTAAAACGG‑CTGCCAACGGACACCGGACAGCAAAACATCGGTTTCACGAATGTGTTGACg                                                                                                                                                                                               >  2:2656871/1‑289 (MQ=255)
                                                                                          gacataaagagccAGTTCGTGCGGCGGTGTCATCATGATACGGAACGTTCTGTCCTTGTCGTGACTTTTGCGTTTTTTGCCGTTTTGCGA‑CACAAACATCACCGGA‑GGGAGCGGGTCGATAAGGCGTACCAC‑CACAAAAAGCAGCGGGCCCTGGGTAGCGCAGGCAAAACCGTGGGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTccgccgc                                                                                                                                                                                <  2:2605079/277‑1 (MQ=255)
                                                                                          cACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTccgccgc                                                                                                                                                                                <  1:2325871/288‑1 (MQ=255)
                                                                                           aCGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTccgccgcc                                                                                                                                                                               >  2:1203292/1‑288 (MQ=255)
                                                                                                  aGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCtcatc                                                                                                                                                                       >  1:1538323/1‑289 (MQ=255)
                                                                                                  aGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCtcat                                                                                                                                                                        >  1:2682825/1‑288 (MQ=255)
                                                                                                   gCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCttt                                                                                                                                                                                                                                                                             <  2:1325225/187‑1 (MQ=255)
                                                                                                    cccAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCtcatca                                                                                                                                                                      >  1:475417/1‑288 (MQ=255)
                                                                                                       aGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGGTGACGACTATGTTTCGCCGCCGCCTCAtcactc                                                                                                                                                                   >  1:1602346/1‑288 (MQ=255)
                                                                                                         ttCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGTCCAGGCAAACATCGCGTTCAGGAATGTGTTTACGAATATGTTCCGCCGCCACCTCAtcactcac                                                                                                                                                                 >  2:1856185/1‑288 (MQ=255)
                                                                                                         ttCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACATTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGCCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTCTTCGATACGCCAGCCAGCTAAAATGG‑CTGACAAGGGAAACCGGCAAGTAAAACATCGTGTTCAGGAATGTGGTGACGAAAATGTTCCGCCGCCGCGTCAtcccccac                                                                                                                                                                 >  2:847084/1‑288 (MQ=255)
                                                                                                          tCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACg                                                                                                                                                                <  1:1203292/288‑1 (MQ=255)
                                                                                                           cGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGcc                                                                                                                                                              <  1:2506008/289‑1 (MQ=255)
                                                                                                              ccAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCctt                                                                                                                                                            <  1:2665671/288‑1 (MQ=255)
                                                                                                                  cGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCCAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCCCTCACGCCTTCTTTa                                                                                                                                                       >  1:1346579/1‑289 (MQ=255)
                                                                                                                  cGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTa                                                                                                                                                       >  1:379906/1‑289 (MQ=255)
                                                                                                                   gTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGc                                                                                                                                                                                                                                <  2:2684081/215‑1 (MQ=255)
                                                                                                                      tGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCttt                                                                                                                                                        <  1:1054577/284‑1 (MQ=255)
                                                                                                                      tGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CCGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCttt                                                                                                                                                        >  2:1054577/1‑284 (MQ=255)
                                                                                                                             tgatgCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAggg                                                                                                                                             <  1:94550/288‑1 (MQ=255)
                                                                                                                                tgCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGAt                                                                                                                                         >  1:550606/1‑289 (MQ=255)
                                                                                                                                  cGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGc                                                                                                                                                                                                                                >  2:2702220/1‑200 (MQ=255)
                                                                                                                                       cGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCgg                                                                                                                                  >  2:384432/1‑289 (MQ=255)
                                                                                                                                        gTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGa                                                                                                                                 <  1:2376227/289‑1 (MQ=255)
                                                                                                                                            tACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATc                                                                                                                             <  1:1419607/289‑1 (MQ=255)
                                                                                                                                                  ggTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGc                                                                                                                                                                                                                                <  2:591158/184‑1 (MQ=255)
                                                                                                                                                   gTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATgg                                                                                                                                                    >  1:2312006/1‑259 (MQ=255)
                                                                                                                                                   gTCGTGACTTTTCAGGTTTTTGACGTTTA‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGa                                                                                                                                                                                                 <  2:1828535/214‑1 (MQ=255)
                                                                                                                                                   gTCGTGACTTTTCAGGTTTTTGACGTTTA‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGa                                                                                                                                                                                                 >  1:1828535/1‑214 (MQ=255)
                                                                                                                                                        gACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGAAGCGCGTCGAGAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGATCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTCAAACATCGTGTTCCGGAATGTGTTGACGAATATGTTCCGACGCCGCCTCATCACTCACACCTTCTTTATGGCTGCGGTGGATGTAGCCGCTAACTTCATTAAAatt                                                                                                                 >  2:2090392/1‑288 (MQ=255)
                                                                                                                                                           ttttCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGCGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAatgatg                                                                                                              <  2:24497/289‑1 (MQ=255)
                                                                                                                                                             ttCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTACAATGATGCg                                                                                                            >  1:1260123/1‑289 (MQ=255)
                                                                                                                                                              tCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGa                                                                                                                                          >  2:1890711/1‑258 (MQ=255)
                                                                                                                                                                   tttttGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCg                                                                                                      <  2:1990120/289‑1 (MQ=255)
                                                                                                                                                                      ttgccgtcat‑CGCGCAAAAACAGCGGCGGA‑CGACGCGGCTCGATAGTGCGTACCAC‑AGCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGAt                                                                                                   <  2:2380271/280‑1 (MQ=255)
                                                                                                                                                                      ttGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGAt                                                                                                   <  1:2633245/289‑1 (MQ=255)
                                                                                                                                                                            gttttagataccaAGCATCTCCACA‑CGACGCGCGTCCATAATCAGAACCAC‑ATCAAAAAACCGCGTGGCCTTGTAACCGCAGCCAAAACCGCTCGCCGGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGACAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCaa                                                                                              <  2:1602346/278‑1 (MQ=255)
                                                                                                                                                                               t‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCaataat                                                                                          >  1:416308/1‑289 (MQ=255)
                                                                                                                                                                                  gAGCACAAACATCCACGGA‑CGACCGGCGTCGATAATGCGTACCAC‑ATCAAACACCAGCGTGCCCTGAGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATgg                                                                                        <  2:25916/289‑1 (MQ=255)
                                                                                                                                                                                     cacaAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGtt                                                                                      <  2:710601/288‑1 (MQ=255)
                                                                                                                                                                                      acacacacCGCCGGA‑CGACGCGCGACGATACTGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGtt                                                                                      <  2:1260123/287‑1 (MQ=255)
                                                                                                                                                                                      acacacATCGCCGGG‑AGACACGACTGGATGAAGCTTAACCA‑ATAAAAAACCATCGGGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCACGAGCGATTTCTGTTGCGATAAGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAGCCGGCCAGTAATACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGTTATTTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTgg                                                                                    <  2:2215095/289‑1 (MQ=255)
                                                                                                                                                                                      acaAACATCGCCGGA‑CGACGGGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTgg                                                                                    <  1:1286498/289‑1 (MQ=255)
                                                                                                                                                                                      acaAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGtt                                                                                      <  2:948538/287‑1 (MQ=255)
                                                                                                                                                                                      acaAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTgg                                                                                    <  2:550606/289‑1 (MQ=255)
                                                                                                                                                                                      acaAACAGCGCCGGA‑CGACGAGCGTAGATAATGCGTACCAC‑AGCAAAAAACAGCGTGCCCTGGGTATCGCAAGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGtt                                                                                      <  2:2128392/287‑1 (MQ=255)
                                                                                                                                                                                           cATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTta                                                                               <  2:888815/289‑1 (MQ=255)
                                                                                                                                                                                             tCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTtata                                                                             <  1:2049357/289‑1 (MQ=255)
                                                                                                                                                                                                         cgcgcgTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGa                                                                                                                                                                                                 >  1:786350/1‑162 (MQ=255)
                                                                                                                                                                                                         cgcgcgTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGa                                                                                                                                                                                                 <  2:786350/162‑1 (MQ=255)
                                                                                                                                                                                                              gTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTg                                                                                                                                           <  2:2301088/211‑1 (MQ=255)
                                                                                                                                                                                                               tCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGTTTTATAAGTGCGTACCGCAggtt                                                            >  1:1407795/1‑289 (MQ=255)
                                                                                                                                                                                                                      tGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCAtcact                                                                                                                                                                    <  1:2289614/178‑1 (MQ=255)
                                                                                                                                                                                                                         gTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCAtt                                                   <  1:1483842/288‑1 (MQ=255)
                                                                                                                                                                                                                              ac‑aTCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTgg                                             <  1:1440986/289‑1 (MQ=255)
                                                                                                                                                                                                                                atccAAAAAAC‑GCGTGCCCTGGGTATCACAGGCAAAGCCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGAGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTGCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTTGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGTATGTTTGTTCCATTCGCTGGTa                                           <  2:475417/286‑1 (MQ=255)
                                                                                                                                                                                                                                 aTCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGtt                                                                                      <  1:2211102/246‑1 (MQ=255)
                                                                                                                                                                                                                                      aaaaCAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAacac                                      >  1:2698515/1‑289 (MQ=255)
                                                                                                                                                                                                                                       aaaCAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACg                                     <  1:2499446/289‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGc                                                                                                                                                                                                                                <  1:1726309/96‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGc                                                                                                                                                                                                                                >  2:1726309/1‑96 (MQ=255)
                                                                                                                                                                                                                                               tGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCa                                   >  1:1675827/1‑283 (MQ=255)
                                                                                                                                                                                                                                               tGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGGTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTcc                             >  1:2452071/1‑289 (MQ=255)
                                                                                                                                                                                                                                                  ccTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCg                                                                  <  1:1564133/249‑1 (MQ=255)
                                                                                                                                                                                                                                                  ccTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCg                                                                  >  2:1564133/1‑249 (MQ=255)
                                                                                                                                                                                                                                                     gggTATCGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCGTTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAAt                       <  1:969928/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           cGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTTGCTGGTAAACACGCACTGTCCGCCCAtcgcttc                 >  2:361972/1‑283 (MQ=255)
                                                                                                                                                                                                                                                           cGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACg                                     >  1:1966182/1‑269 (MQ=255)
                                                                                                                                                                                                                                                           cGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAAtcgattc                 >  1:88203/1‑283 (MQ=255)
                                                                                                                                                                                                                                                           cGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAAtcgattc                 >  1:1768784/1‑283 (MQ=255)
                                                                                                                                                                                                                                                           cGCAGGCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CGGACAAGGGAAACCGGCCAGAAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATAACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTGGCCGGATCGAAATAATGGTTGGCTTTATAAGTGCGTACGGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATGcatc                  >  2:1942789/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                gCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATc                                                                                                                                                                                                                    <  2:1475881/89‑1 (MQ=255)
                                                                                                                                                                                                                                                                gCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTTGCTTTATAAGTGCGTACCGCATGTTTGGTCCATTCGCTGGTAAACACGCAATGTCCGCCCAtcgaatcaaaa             >  2:1387030/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                gCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCaaaa             >  1:1902294/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                gCAAAACCGTGCGCCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGCGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCACTTGCTGGTAACCACGCACTGTCCGCCAACCCATTCaaaa             >  2:1481795/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                          gcgcCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATc                                                                                                                             <  2:2324259/166‑1 (MQ=255)
                                                                                                                                                                                                                                                                          gcgcCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGtt                                                                                      >  1:2266627/1‑205 (MQ=255)
                                                                                                                                                                                                                                                                          gcgcCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGtt                                                                                      >  2:2502763/1‑205 (MQ=255)
                                                                                                                                                                                                                                                                          gcgcCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGAt  <  1:2415882/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                          gcgcCCGCCCGAGCGAGTTCTTTTTCGATACGCCAGCCAGCGAAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGAt  <  1:1019325/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                  
CACCAGCACGATGCGTTCACGGTGCTGCGGCAGAAAATGTTTGCCGTCGATGATTTTCGGATCGTCTGGCCCATTATCTTCTGCATCAGCCACGTCATAGCCCAGTTCGTCCAGCGTCTGCATGATGATGCGGAACGTTTTACCCTGGTCGTGACTTTTCAGGTTTTTGACGTTTT‑CGAGCACAAACATCGCCGGA‑CGACGCGCGTCGATAATGCGTACCAC‑ATCAAAAAACAGCGTGCCCTGGGTATCGCAGGCAAAACCGTGCGCCCGCCCGAGGCTGTTCTTTTTGCTTACGCCAGCCAGGCTAAATGG‑CTGACAAGGGAAACCGGCCAGTAAAACATCGTGTTCAGGAATGTGTTGACGAATATGTTCCGCCGCCGCCTCATCACTCACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATGCTTTCAAAACCGCGACGGAT  >  CP042583/1668995‑1669546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A