Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,963,829	C→T	R134H (CGC→CAC)	gpp ←	guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,963,829	0	C	T	100.0%	95.5 / NA	30	R134H (CGC→CAC)	gpp	guanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/14); total (16/14)

GATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963701‑3963963

gATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGc                                                                                                                              <  1:404821/139‑1 (MQ=255)

  ttACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcac                                                                                                                            <  2:186624/139‑1 (MQ=255)

  ttACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcac                                                                                                                            <  1:755950/139‑1 (MQ=255)

     cGATCGGCTAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcaccac                                                                                                                         >  1:688496/1‑139 (MQ=255)

       aTCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       <  2:168850/139‑1 (MQ=255)

         cGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAgt                                                                                                                     <  2:569947/139‑1 (MQ=255)

           gCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAgtgg                                                                                                                   >  1:35785/1‑139 (MQ=255)

            cAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAgtggt                                                                                                                  <  1:126612/139‑1 (MQ=255)

                  aGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGc                                                                                                            <  1:360558/139‑1 (MQ=255)

                   gCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCa                                                                                                           <  2:35785/139‑1 (MQ=255)

                                        gCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGt                                                                                      <  1:38672/139‑1 (MQ=255)

                                               gACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCct                                                                               >  1:771025/1‑139 (MQ=255)

                                                   ggCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTc                                                                           >  2:44655/1‑139 (MQ=255)

                                                    gCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCa                                                                          <  1:44655/139‑1 (MQ=255)

                                                                     tttGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTAc                                                         >  2:173711/1‑139 (MQ=255)

                                                                       tgtgCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATc                                                                 >  2:509851/1‑129 (MQ=255)

                                                                       tgtgCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATc                                                                 <  1:509851/129‑1 (MQ=255)

                                                                               cGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCg                                               >  2:696313/1‑139 (MQ=255)

                                                                               cGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCg                                               <  2:688496/139‑1 (MQ=255)

                                                                               cGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCA‑CAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCga                                              >  1:745264/1‑139 (MQ=255)

                                                                                      gTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAAttt                                        >  2:520982/1‑139 (MQ=255)

                                                                                                  gTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTgg                            >  2:530968/1‑139 (MQ=255)

                                                                                                   tACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGc                           >  2:66754/1‑139 (MQ=255)

                                                                                                    aCTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCa                          <  2:119703/139‑1 (MQ=255)

                                                                                                        gCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATaa                      >  2:430626/1‑139 (MQ=255)

                                                                                                        acTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATaa                      >  1:499761/2‑139 (MQ=255)

                                                                                                                  tatCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGc            <  1:66754/139‑1 (MQ=255)

                                                                                                                         caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACg     >  1:37547/1‑139 (MQ=255)

                                                                                                                          accaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACgg    >  1:106552/1‑139 (MQ=255)

                                                                                                                            caGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCa  >  1:739684/1‑139 (MQ=255)

GATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963701‑3963963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A