Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,963,829	C→T	R134H (CGC→CAC)	gpp ←	guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,963,829	0	C	T	100.0%	177.9 / NA	55	R134H (CGC→CAC)	gpp	guanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/28); total (27/28)

CCCCAGATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963696‑3963963

ccccAGATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGa                                                                                                                                   >  2:989098/1‑139 (MQ=255)

ccccAGATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGa                                                                                                                                   <  2:1008226/139‑1 (MQ=255)

         aCGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGTCCATCGACAGGCTGAACAACGAGTTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcacca                                                                                                                          <  1:1027369/139‑1 (MQ=255)

           gATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCaccacc                                                                                                                        >  1:1216468/1‑139 (MQ=255)

            aTCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       <  1:49551/139‑1 (MQ=255)

                gCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAgtgg                                                                                                                   >  2:781398/1‑139 (MQ=255)

                gCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAgtgg                                                                                                                   >  1:219262/1‑139 (MQ=255)

                        gCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCa                                                                                                           <  2:1216468/139‑1 (MQ=255)

                         cGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCaa                                                                                                          >  2:735681/1‑139 (MQ=255)

                         cGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCaa                                                                                                          >  1:844438/1‑139 (MQ=255)

                            tCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGGGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  1:678190/139‑1 (MQ=255)

                              cagccagGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGccc                                                                                                     >  2:318921/1‑139 (MQ=255)

                                      tggcGCAGCCCCTCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGc                                                                                                            >  1:933405/4‑124 (MQ=255)

                                      tGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGc                                                                                                            <  2:933405/124‑1 (MQ=255)

                                      tGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAAt                                                                                             >  1:541443/1‑139 (MQ=255)

                                          gCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGa                                                                                                                                   <  2:1436567/97‑1 (MQ=255)

                                          gCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGa                                                                                                                                   >  1:1436567/1‑97 (MQ=255)

                                                       aGGCTTAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCATCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCtt                                                                            <  2:1003279/139‑1 (MQ=255)

                                                       aGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCtt                                                                            <  2:534503/139‑1 (MQ=255)

                                                        ggCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGc                                                                                                            >  1:663892/1‑106 (MQ=255)

                                                        ggCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGc                                                                                                            <  2:663892/106‑1 (MQ=255)

                                                         gCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGc                                                                                                            <  2:1452584/105‑1 (MQ=255)

                                                         gCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGc                                                                                                            >  1:1452584/1‑105 (MQ=255)

                                                         gCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCa                                                                          >  1:540787/1‑139 (MQ=255)

                                                          cTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  1:625201/109‑1 (MQ=255)

                                                          cTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       >  2:625201/1‑109 (MQ=255)

                                                            gAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCg                                                                       <  1:989098/139‑1 (MQ=255)

                                                               caacGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTg                                                                    <  1:735681/139‑1 (MQ=255)

                                                               caacGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTg                                                                    <  1:781398/139‑1 (MQ=255)

                                                               caacGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTg                                                                    <  1:833641/139‑1 (MQ=255)

                                                               caacGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTg                                                                    >  2:1526415/1‑139 (MQ=255)

                                                                             gtgCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCgg                                                      <  2:541443/139‑1 (MQ=255)

                                                                                       gCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCgaga                                            <  2:844438/139‑1 (MQ=255)

                                                                                           gTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       >  2:33547/1‑76 (MQ=255)

                                                                                           gTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  1:33547/76‑1 (MQ=255)

                                                                                             tACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       >  1:423740/1‑58 (MQ=255)

                                                                                             tACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       <  2:423740/58‑1 (MQ=255)

                                                                                             tACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTcc                                      >  2:1189254/1‑139 (MQ=255)

                                                                                               ccAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCAc                                                                         >  2:1597285/1‑102 (MQ=255)

                                                                                               ccAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCAc                                                                         <  1:1597285/102‑1 (MQ=255)

                                                                                                   ttCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       >  2:281273/1‑52 (MQ=255)

                                                                                                   ttCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       <  1:281273/52‑1 (MQ=255)

                                                                                                    tCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCtt                               <  1:318921/139‑1 (MQ=255)

                                                                                                      aGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATc                                                                                            <  1:669595/76‑1 (MQ=255)

                                                                                                      aGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATc                                                                                            >  2:669595/1‑76 (MQ=255)

                                                                                                        tACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               >  2:1492689/1‑103 (MQ=255)

                                                                                                        tACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               <  1:1492689/103‑1 (MQ=255)

                                                                                                        tACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGc                           >  2:136240/1‑139 (MQ=255)

                                                                                                             gCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCtt                               >  2:1473447/1‑130 (MQ=255)

                                                                                                             gCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCtt                               <  1:1473447/130‑1 (MQ=255)

                                                                                                               tCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATaaaa                    >  1:525152/1‑139 (MQ=255)

                                                                                                                ccGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAAt                   >  1:1230912/1‑139 (MQ=255)

                                                                                                                     tataTCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCAccc              <  2:219262/139‑1 (MQ=255)

                                                                                                                                 caGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCa  >  1:776524/1‑139 (MQ=255)

                                                                                                                                 caGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCa  <  1:1189254/139‑1 (MQ=255)

CCCCAGATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963696‑3963963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A