Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,963,829	C→T	R134H (CGC→CAC)	gpp ←	guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,963,829	0	C	T	100.0%	141.0 / NA	44	R134H (CGC→CAC)	gpp	guanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/23); total (21/23)

GATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963701‑3963963

gATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGc                                                                                                                              <  1:312500/139‑1 (MQ=255)

  ttACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcac                                                                                                                            <  2:316420/139‑1 (MQ=255)

  ttACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcac                                                                                                                            <  1:616011/139‑1 (MQ=255)

       aTCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       <  1:824422/139‑1 (MQ=255)

        tCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAg                                                                                                                      <  2:865107/139‑1 (MQ=255)

           gCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAgtgg                                                                                                                   >  2:253893/1‑139 (MQ=255)

               aaTAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGtgtg                                                                                                               >  1:377980/1‑139 (MQ=255)

                    cGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGTTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       <  2:427202/126‑1 (MQ=255)

                    cGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAAGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCAccacca                                                                                                                       >  1:427202/1‑126 (MQ=255)

                       tCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  2:210096/139‑1 (MQ=255)

                       tCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  1:993925/139‑1 (MQ=255)

                               ggTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATaa                                                                                               >  2:944859/1‑139 (MQ=255)

                                gTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATaaa                                                                                              >  2:351594/1‑139 (MQ=255)

                                   aCGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCa                                                                                           >  1:520008/1‑139 (MQ=255)

                                         cccATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGc                                                                                                                              >  2:775488/1‑98 (MQ=255)

                                         cccATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGc                                                                                                                              <  1:775488/98‑1 (MQ=255)

                                           cATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  1:913390/119‑1 (MQ=255)

                                           cATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       >  2:913390/1‑119 (MQ=255)

                                             tCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTc                                                                                 >  2:943136/1‑139 (MQ=255)

                                              cGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACAAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTcc                                                                                >  2:676288/1‑139 (MQ=255)

                                                     cTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCAc                                                                         <  1:484420/139‑1 (MQ=255)

                                                           aacGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGa                                                                   <  1:676288/139‑1 (MQ=255)

                                                           aacGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGa                                                                   <  2:498742/139‑1 (MQ=255)

                                                              gAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCa                                                                >  2:102606/1‑139 (MQ=255)

                                                               aGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               <  1:253893/139‑1 (MQ=255)

                                                                  tggtTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTg                                                            <  1:944859/139‑1 (MQ=255)

                                                                                 tGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCGCCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCgag                                             >  2:1049086/1‑139 (MQ=255)

                                                                                   ccGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCaa                                                                                                          <  1:465189/76‑1 (MQ=255)

                                                                                   ccGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCaa                                                                                                          >  2:465189/1‑76 (MQ=255)

                                                                                        tACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTcc                                      >  1:190432/1‑139 (MQ=255)

                                                                                            aGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTgcg                                  >  2:488490/1‑139 (MQ=255)

                                                                                                     cTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcac                                                                                                                            >  2:502006/1‑40 (MQ=255)

                                                                                                     cTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcac                                                                                                                            <  1:502006/40‑1 (MQ=38)

                                                                                                             gCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGa                                                                   >  1:883599/1‑89 (MQ=255)

                                                                                                             gCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGa                                                                   <  2:883599/89‑1 (MQ=255)

                                                                                                                    tCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCAt          <  2:190432/139‑1 (MQ=255)

                                                                                                                        ccaccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGa                                                     <  2:609527/92‑1 (MQ=255)

                                                                                                                        ccaccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGa                                                     >  1:609527/1‑92 (MQ=255)

                                                                                                                         caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       >  2:582657/1‑41 (MQ=255)

                                                                                                                         caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  1:582657/41‑1 (MQ=255)

                                                                                                                         caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGc                                                                                    >  2:308054/1‑60 (MQ=255)

                                                                                                                         caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGc                                                                                    <  1:308054/60‑1 (MQ=255)

                                                                                                                         caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACg     >  2:1033622/1‑139 (MQ=255)

                                                                                                                            caGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCa  <  2:520008/139‑1 (MQ=255)

GATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963701‑3963963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A