Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 766 64.2 3232166 90.9% 2938038 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,826,151 T→C I314I (ATT→ATC)  recG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,826,1510TC100.0% 194.2 / NA 54I314I (ATT→ATC) recGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/31);  total (23/31)

CGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAA  >  NC_000913/3826018‑3826282
                                                                                                                                     |                                                                                                                                   
cGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCa                                                                                                                                <  1:292212/139‑1 (MQ=255)
cGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCa                                                                                                                                >  2:280580/1‑139 (MQ=255)
      cacaGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaa                                                                                                                          <  1:280580/139‑1 (MQ=255)
        caGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAAc                                                                                                                        <  1:144180/139‑1 (MQ=255)
           gCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAgg                                                                                                                     >  1:611541/1‑139 (MQ=255)
              cgcgTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAg                                                                                                                  >  2:1593290/1‑139 (MQ=255)
                cgTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGccc                                                                                                                                 <  1:31304/122‑1 (MQ=255)
                cgTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGccc                                                                                                                                 >  2:31304/1‑122 (MQ=255)
                     tGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGAt                                                                                                           >  2:669358/1‑139 (MQ=255)
                     tGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGAt                                                                                                           >  2:1101736/1‑139 (MQ=255)
                        cGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGc                                                                                                        <  1:1593290/139‑1 (MQ=255)
                              tCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAAc                                                                                                  <  2:1279407/139‑1 (MQ=255)
                                     cgATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTa                                                                                           >  2:861960/1‑139 (MQ=255)
                                       atatGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGcc                                                                                                                                  >  1:335233/1‑98 (MQ=255)
                                       atatGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGcc                                                                                                                                  <  2:335233/98‑1 (MQ=255)
                                       atatGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaa                                                                                                                          >  1:1263877/1‑106 (MQ=255)
                                       atatGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaa                                                                                                                          <  2:1263877/106‑1 (MQ=255)
                                                  gATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGcc                                                                                                                                  <  1:765184/87‑1 (MQ=255)
                                                  gATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGcc                                                                                                                                  >  2:765184/1‑87 (MQ=255)
                                                  gATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaaa                                                                                                                         <  2:124656/96‑1 (MQ=255)
                                                  gATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaaa                                                                                                                         >  1:124656/1‑96 (MQ=255)
                                                   aTGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAgcagca                                                                             <  1:669358/139‑1 (MQ=255)
                                                       gCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGcc                                                                         >  2:146552/1‑139 (MQ=255)
                                                          gatgatGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAAt                                                                      <  2:1152652/139‑1 (MQ=255)
                                                           atgatgCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATa                                                                     <  2:796160/139‑1 (MQ=255)
                                                                     tGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                           <  1:565407/139‑1 (MQ=255)
                                                                     tGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                           <  1:909468/139‑1 (MQ=255)
                                                                     tGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                           <  2:1324063/139‑1 (MQ=255)
                                                                     tGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                           <  2:905920/139‑1 (MQ=255)
                                                                          cAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGt                                                      <  1:1118320/139‑1 (MQ=255)
                                                                                gATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCAc                                                <  1:1256029/139‑1 (MQ=255)
                                                                                 aTGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCa                                                                        >  1:1253442/1‑114 (MQ=255)
                                                                                 aTGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCa                                                                        <  2:1253442/114‑1 (MQ=255)
                                                                                  tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAAt                                                                      >  1:1260503/1‑115 (MQ=255)
                                                                                  tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAAt                                                                      <  2:1260503/115‑1 (MQ=255)
                                                                                  tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCg                                              >  2:917308/1‑139 (MQ=255)
                                                                                  tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCg                                              <  1:1101736/139‑1 (MQ=255)
                                                                                  tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCg                                              <  1:861960/139‑1 (MQ=255)
                                                                                    tAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCt                                            <  1:1436493/139‑1 (MQ=255)
                                                                                           cGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCa                                                                                                                           >  2:1160391/1‑53 (MQ=255)
                                                                                           cGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCa                                                                                                                           <  1:1160391/53‑1 (MQ=255)
                                                                                           cGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGagcag                                                                               <  1:1007277/97‑1 (MQ=255)
                                                                                           cGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGagcag                                                                               >  2:1007277/1‑97 (MQ=255)
                                                                                                            ccgccCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGggctgg                         >  2:1105997/1‑134 (MQ=255)
                                                                                                            ccgccCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGggctgg                         <  1:1105997/134‑1 (MQ=255)
                                                                                                             cgccCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACCCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGcc                   >  1:1361980/1‑139 (MQ=255)
                                                                                                             cgccCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATAGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGcc                   >  1:1560217/1‑139 (MQ=255)
                                                                                                                ccTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGt                <  2:1475405/139‑1 (MQ=255)
                                                                                                                      cgcgTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAg          <  1:210195/139‑1 (MQ=255)
                                                                                                                            gcgtgcgATCGCCCACGGCAAACAGGTAGCATTGATTGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCt                                            <  1:1053867/99‑1 (MQ=255)
                                                                                                                            gcgtgcgATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCt                                            >  2:1053867/1‑99 (MQ=255)
                                                                                                                              gtgcgATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTaa  >  2:1360001/1‑139 (MQ=255)
                                                                                                                               tgcgATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                           >  2:1052041/1‑81 (MQ=255)
                                                                                                                               tgcgATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                           <  1:1052041/81‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAA  >  NC_000913/3826018‑3826282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A