Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,826,151	T→C	I314I (ATT→ATC)	recG →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,826,151	0	T	C	100.0%	189.6 / NA	54	I314I (ATT→ATC)	recG	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/26); total (28/26)

CAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAG  >  NC_000913/3826015‑3826271

cAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGc                                                                                                                        >  1:970153/1‑139 (MQ=255)

         cacaGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaa                                                                                                               <  1:1479402/139‑1 (MQ=255)

                      aGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaa                                                                                                               <  2:706412/126‑1 (MQ=255)

                      aGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCaa                                                                                                               >  1:706412/1‑126 (MQ=255)

                        tGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGAt                                                                                                >  2:199968/1‑139 (MQ=255)

                                aTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCATGGCAAACAGGTAGCATTGATGGCACCaa                                                                                        >  1:1296317/1‑139 (MQ=255)

                                  cGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCATGGCAAACAGGTAGCATTGATGGCACCAAcc                                                                                      <  2:1296317/139‑1 (MQ=255)

                                    aGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGa                                                                                    >  2:1169610/1‑139 (MQ=255)

                                                     gATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTa                                                                                                        <  2:668447/102‑1 (MQ=255)

                                                     gATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTa                                                                                                        >  1:668447/1‑102 (MQ=255)

                                                      aTGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAgcagca                                                                  <  2:970153/139‑1 (MQ=255)

                                                       tgtgCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGag                                                                       >  1:1189435/1‑133 (MQ=255)

                                                       tgtgCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGag                                                                       <  2:1189435/133‑1 (MQ=255)

                                                       tgtgCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCAc                                                                 >  2:1440423/1‑139 (MQ=255)

                                                          gCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAAc                                                                                                             >  2:465729/1‑92 (MQ=255)

                                                          gCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAAc                                                                                                             <  1:465729/92‑1 (MQ=255)

                                                          gCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAgcagca                                                                  >  2:689220/1‑135 (MQ=255)

                                                          gCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAgcagca                                                                  <  1:689220/135‑1 (MQ=255)

                                                               tgatgCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCATCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATaa                                                         >  2:1168514/1‑139 (MQ=255)

                                                                 atgCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGccc                                                                                                                      <  2:529521/76‑1 (MQ=255)

                                                                 atgCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGccc                                                                                                                      >  1:529521/1‑76 (MQ=255)

                                                                 atgCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACt                                                       >  1:1166861/1‑139 (MQ=255)

                                                                      tCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGc                                                  >  2:226508/1‑139 (MQ=255)

                                                                        tGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                <  1:1440423/139‑1 (MQ=255)

                                                                        tGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                <  1:1290009/139‑1 (MQ=255)

                                                                        tGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                <  1:199968/139‑1 (MQ=255)

                                                                                ggCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTg                                        >  2:1266649/1‑139 (MQ=255)

                                                                                ggCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTg                                        <  1:226508/139‑1 (MQ=255)

                                                                                ggCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTg                                        >  2:1081840/1‑139 (MQ=255)

                                                                                ggCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACACCAATAACTTCCGCAACTGGTTTg                                        >  1:444431/1‑139 (MQ=255)

                                                                                    aTGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCAcc                                    >  1:1057159/1‑139 (MQ=255)

                                                                                     tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACt                                                       <  2:1157615/119‑1 (MQ=255)

                                                                                     tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACt                                                       >  1:1157615/1‑119 (MQ=255)

                                                                                     tGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCg                                   >  1:808906/1‑139 (MQ=255)

                                                                                       tAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCt                                 <  2:1166861/139‑1 (MQ=255)

                                                                                        aGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTc                                <  1:1081840/139‑1 (MQ=255)

                                                                                          gTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCAc                                                                                                                    <  2:55740/53‑1 (MQ=255)

                                                                                          gTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCAc                                                                                                                    >  1:55740/1‑53 (MQ=255)

                                                                                            tCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTa                            >  1:849027/1‑139 (MQ=255)

                                                                                               ggTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCAc                                                                                                                    <  1:1519884/48‑1 (MQ=255)

                                                                                               ggTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCAc                                                                                                                    >  2:1519884/1‑48 (MQ=255)

                                                                                               ggTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACTGGTAGCATTGATGGCACCAACCGAATTGCTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                <  2:4126/116‑1 (MQ=255)

                                                                                               ggTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAAATACTCGCCGAGCAGCACGCCAATAACTTCCGCaa                                                >  1:4126/1‑116 (MQ=255)

                                                                                                gTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACgg                                                                                                                  <  1:1124591/49‑1 (MQ=255)

                                                                                                gTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACgg                                                                                                                  >  2:1124591/1‑49 (MQ=255)

                                                                                                gTAAAACGCTGGTCACCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGa                        >  1:932633/1‑139 (MQ=255)

                                                                                                   aaaCGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGc                                                                                                                        >  1:328482/1‑40 (MQ=255)

                                                                                                       gCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGggc                 <  1:1266649/139‑1 (MQ=255)

                                                                                                           gTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTAt                           >  2:295724/1‑125 (MQ=255)

                                                                                                           gTCGCCGCCCTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTAt                           <  1:295724/125‑1 (MQ=255)

                                                                                                                    cTCGCCGCGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTa    <  2:849027/139‑1 (MQ=255)

                                                                                                                      cgccgcGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAg  <  2:46867/139‑1 (MQ=255)

                                                                                                                      cgccgcGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAg  <  2:1057159/139‑1 (MQ=255)

                                                                                                                      cgccgcGTTGCGTGCGATCGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACACCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAg  <  2:444431/139‑1 (MQ=255)

CAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCCCACGGCAAACAGGTAGCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAG  >  NC_000913/3826015‑3826271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A