Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,171,879	C→T	111 (TAG→TAA)	ygiZ ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,171,879	0	C	T	100.0%	109.7 / NA	35	111 (TAG→TAA)	ygiZ	inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/20); total (15/20)

ATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATA  >  NC_000913/3171744‑3171999

aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTaa                                                                                                                       <  2:1140573/139‑1 (MQ=255)

  tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATa                                                                                                                     >  1:839259/1‑139 (MQ=255)

    tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGc                                                                                                                   <  1:898533/139‑1 (MQ=255)

    tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGCAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTTACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGc                                                                                                                   <  2:246554/139‑1 (MQ=255)

      cAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                 <  2:445815/139‑1 (MQ=255)

       aGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCaaa                                                                                                                >  2:29915/1‑139 (MQ=255)

                             catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                          >  1:1086128/1‑139 (MQ=255)

                                gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                 >  1:198936/1‑113 (MQ=255)

                                gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                 <  2:198936/113‑1 (MQ=255)

                                  aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                     <  2:839259/139‑1 (MQ=255)

                                      tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacc                                                                                 <  2:1086128/139‑1 (MQ=255)

                                                  tGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAGTAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACa                                                                     >  2:773169/1‑139 (MQ=255)

                                                               ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                        <  1:734749/139‑1 (MQ=255)

                                                               ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                        <  1:827116/139‑1 (MQ=255)

                                                                 aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                            <  1:757379/133‑1 (MQ=255)

                                                                 aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTAATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                            >  2:757379/1‑133 (MQ=255)

                                                                                    tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCGCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGGGCGAGAATACACATACCCAAAACTCg                                   <  1:877887/139‑1 (MQ=255)

                                                                                      gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga                                                         <  1:173922/115‑1 (MQ=255)

                                                                                      gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga                                                         >  2:173922/1‑115 (MQ=255)

                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                 <  1:1104028/37‑1 (MQ=255)

                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                 >  2:1104028/1‑37 (MQ=255)

                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                                                                      <  2:430142/64‑1 (MQ=255)

                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                                                                      >  1:430142/1‑64 (MQ=255)

                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                                                                              >  1:708063/1‑72 (MQ=255)

                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                                                                              <  2:708063/72‑1 (MQ=255)

                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt           >  1:940355/1‑139 (MQ=255)

                                                                                                               ttAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt        <  2:940355/139‑1 (MQ=255)

                                                                                                                tAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTta       >  1:790347/1‑139 (MQ=255)

                                                                                                                     cGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata  <  2:626768/139‑1 (MQ=255)

                                                                                                                       aCTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt        <  1:980954/131‑1 (MQ=255)

                                                                                                                       aCTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt        >  2:980954/1‑131 (MQ=255)

                                                                                                                         tctcATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                <  2:376557/105‑1 (MQ=255)

                                                                                                                         tctcATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                >  1:376557/1‑105 (MQ=255)

                                                                                                                            cATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                          <  2:653551/76‑1 (MQ=255)

                                                                                                                            cATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                          >  1:653551/1‑76 (MQ=255)

ATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATA  >  NC_000913/3171744‑3171999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A