Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,171,879	C→T	111 (TAG→TAA)	ygiZ ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,171,879	0	C	T	100.0%	113.4 / NA	36	111 (TAG→TAA)	ygiZ	inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/22); total (14/22)

TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAA  >  NC_000913/3171746‑3172006

tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTaa                                                                                                                               <  2:725043/137‑1 (MQ=255)

tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTaa                                                                                                                               >  1:725043/1‑137 (MQ=255)

   ccAGCGAATTGCCAAAATTGATGGCAATAATGGTTGATTTGGGAATGGCGGACAAAGTTGATTTTGAGG‑GAAGGTTAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                                          <  1:299483/139‑1 (MQ=255)

   ccAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                                          <  2:333942/139‑1 (MQ=255)

    cAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                         <  2:636770/139‑1 (MQ=255)

                  aTTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTcc                                                                                                           <  2:522835/139‑1 (MQ=255)

                                          tGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   <  2:791128/139‑1 (MQ=255)

                                             gAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGc                                                                                                                           <  1:276590/96‑1 (MQ=255)

                                             gAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGc                                                                                                                           >  2:276590/1‑96 (MQ=255)

                                                         aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccga                                                                    <  2:47011/139‑1 (MQ=255)

                                                          aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  1:109163/139‑1 (MQ=255)

                                                          aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  1:592679/139‑1 (MQ=255)

                                                           ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                   >  1:812965/1‑107 (MQ=255)

                                                           ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                   <  2:812965/107‑1 (MQ=255)

                                                                aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   >  1:9491/1‑118 (MQ=255)

                                                                aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   <  2:9491/118‑1 (MQ=255)

                                                                                               tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   >  1:36142/1‑102 (MQ=255)

                                                                                               tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  2:36142/102‑1 (MQ=255)

                                                                                               tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                 <  1:75517/136‑1 (MQ=255)

                                                                                               tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                 >  2:75517/1‑136 (MQ=255)

                                                                                                      gagaGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   >  2:674218/1‑95 (MQ=255)

                                                                                                      gagaGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  1:674218/95‑1 (MQ=255)

                                                                                                           ccattAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCAAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccg                                                                     >  2:485337/4‑88 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                         <  1:354714/37‑1 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                         >  2:354714/1‑37 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   <  1:262776/75‑1 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   >  2:262776/1‑75 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgagcgag                                                               <  1:367563/94‑1 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgagcgag                                                               >  2:367563/1‑94 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccg                                                                     <  1:485337/88‑1 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag                      >  1:151013/1‑135 (MQ=255)

                                                                                                           ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag                      <  2:151013/135‑1 (MQ=255)

                                                                                                             gTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                 >  2:92003/1‑139 (MQ=255)

                                                                                                              ttAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt               <  2:167220/139‑1 (MQ=255)

                                                                                                                    cGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat          <  1:92003/139‑1 (MQ=255)

                                                                                                                           cATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataa  >  1:567168/1‑139 (MQ=255)

TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAA  >  NC_000913/3171746‑3172006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A