Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,171,879 |
C→T |
*111* (TAG→TAA) |
ygiZ ← |
inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,171,879 | 0 | C | T | 100.0%
| 103.0
/ NA
| 32 | *111* (TAG→TAA) | ygiZ | inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16) |
TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCT > NC_000913/3171746‑3171992
|
tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATa > 1:420502/1‑139 (MQ=255)
cGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAat > 2:460531/1‑139 (MQ=255)
cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATaaa > 2:315381/1‑139 (MQ=255)
aaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTc < 1:973126/139‑1 (MQ=255)
catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAg < 2:420502/139‑1 (MQ=255)
tGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc < 1:553133/139‑1 (MQ=255)
gggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAcc > 1:737793/1‑139 (MQ=255)
gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa < 1:1061541/97‑1 (MQ=255)
gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa > 2:1061541/1‑97 (MQ=255)
gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga < 2:737793/139‑1 (MQ=255)
aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa < 2:833112/139‑1 (MQ=255)
aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa < 1:460531/139‑1 (MQ=255)
aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc > 2:942859/1‑98 (MQ=255)
aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc < 1:942859/98‑1 (MQ=255)
tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc > 2:68204/1‑87 (MQ=255)
tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc < 1:68204/87‑1 (MQ=255)
aTGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAGCCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa > 1:83943/1‑139 (MQ=255)
aTGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg > 2:58934/1‑95 (MQ=255)
aTGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg < 1:58934/95‑1 (MQ=255)
gagaGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga > 1:924862/1‑139 (MQ=255)
agagCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag < 2:83943/139‑1 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCa < 1:763543/36‑1 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCa > 2:763543/1‑36 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc < 1:811805/75‑1 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc > 2:811805/1‑75 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg < 1:776055/92‑1 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg > 2:776055/1‑92 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag < 2:159508/90‑1 (MQ=255)
ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag > 1:159508/1‑90 (MQ=255)
gTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGGGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCt > 1:750166/1‑139 (MQ=255)
gACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt > 2:221895/1‑112 (MQ=255)
gACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt < 1:221895/112‑1 (MQ=255)
|
TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCT > NC_000913/3171746‑3171992
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
