Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,183,006	G→A	G153S (GGT→AGT)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,183,006	0	G	A	100.0%	121.9 / NA	38	G153S (GGT→AGT)	zupT	zinc transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/16); total (22/16)

AGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATT  >  NC_000913/3182873‑3183137

aGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCtg                                                                                                                                >  1:120247/1‑139 (MQ=255)

  ccATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCtggc                                                                                                                              >  2:763174/1‑139 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                         <  1:157801/136‑1 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                         >  2:157801/1‑136 (MQ=255)

            cACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTACCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGc                                                                                                                    >  2:138791/1‑139 (MQ=255)

                    gCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTa                                                                                                            >  1:206873/1‑139 (MQ=255)

                         aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCgg                                                                                                       >  1:197368/1‑139 (MQ=255)

                            cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                                    <  2:325778/139‑1 (MQ=255)

                                         cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                       >  2:877303/1‑139 (MQ=255)

                                         cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                       >  2:784154/1‑139 (MQ=255)

                                         cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                       >  2:281765/1‑139 (MQ=255)

                                                      cACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                         <  1:1029026/93‑1 (MQ=255)

                                                      cACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                         >  2:1029026/1‑93 (MQ=255)

                                                      cACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCtg                                                                          <  1:763174/139‑1 (MQ=255)

                                                      cACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCtg                                                                          >  2:1149358/1‑139 (MQ=255)

                                                              tCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                                  <  1:1198149/139‑1 (MQ=255)

                                                              tCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                                  <  2:120247/139‑1 (MQ=255)

                                                                        cagcaACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGG‑TTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGAc                                                       >  1:165980/1‑139 (MQ=255)

                                                                                 ggAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                               <  1:1149358/139‑1 (MQ=255)

                                                                                        ggATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATg                                                                                                          <  1:982701/73‑1 (MQ=255)

                                                                                        ggATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATg                                                                                                          >  2:982701/1‑73 (MQ=255)

                                                                                        ggATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtg                                        >  1:882038/1‑139 (MQ=255)

                                                                                           tttGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                     <  1:877303/139‑1 (MQ=255)

                                                                                              ggCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGggggtg                                  >  1:613421/1‑139 (MQ=255)

                                                                                                     cACTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                           <  2:59416/139‑1 (MQ=255)

                                                                                                       cTGG‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGt                         <  2:197368/139‑1 (MQ=255)

                                                                                                          g‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                      >  1:338540/1‑139 (MQ=255)

                                                                                                          g‑CCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                      <  2:338540/139‑1 (MQ=255)

                                                                                                              gTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGTAGGc                                                                                                                    <  2:65454/42‑1 (MQ=255)

                                                                                                              gTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGTAGGc                                                                                                                    >  1:65454/1‑42 (MQ=255)

                                                                                                                     tGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCgg            >  1:779040/1‑139 (MQ=255)

                                                                                                                               cccTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCt                                          >  2:1150076/1‑99 (MQ=255)

                                                                                                                               cccTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCt                                          <  1:1150076/99‑1 (MQ=255)

                                                                                                                               cccTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGAtt  >  2:964162/1‑139 (MQ=255)

                                                                                                                                 cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                           >  2:988672/1‑112 (MQ=255)

                                                                                                                                 cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                           <  1:988672/112‑1 (MQ=255)

                                                                                                                                 cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt               <  2:1096126/124‑1 (MQ=255)

                                                                                                                                 cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt               >  1:1096126/1‑124 (MQ=255)

AGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGG‑CCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATT  >  NC_000913/3182873‑3183137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A