Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,183,006	G→A	G153S (GGT→AGT)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,183,006	0	G	A	100.0%	136.0 / NA	43	G153S (GGT→AGT)	zupT	zinc transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/22); total (21/22)

CGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGG  >  NC_000913/3182891‑3183127

cGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGtt                                                                                                    >  1:1339527/1‑139 (MQ=255)

       aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCgg                                                                                             >  1:1046095/1‑139 (MQ=255)

          cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                          <  2:1339527/139‑1 (MQ=255)

             cATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgg                                                                                       >  2:247516/1‑139 (MQ=255)

                  cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgggg                                                                                     <  2:397504/136‑1 (MQ=255)

                  cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgggg                                                                                     >  1:397504/1‑136 (MQ=255)

                  cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCt                                                                                  >  2:138525/1‑139 (MQ=255)

                  cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCt                                                                                  >  2:1211591/1‑139 (MQ=255)

                   ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGccc                                                                                                        >  2:532628/1‑116 (MQ=255)

                   ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGccc                                                                                                        <  1:532628/116‑1 (MQ=255)

                     cccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                        <  2:299851/130‑1 (MQ=255)

                     cccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                        >  1:299851/1‑130 (MQ=255)

                       cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                         >  2:641503/1‑127 (MQ=255)

                       cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                         <  1:641503/127‑1 (MQ=255)

                                        tttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                               >  2:908680/1‑88 (MQ=255)

                                        tttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                               <  1:908680/88‑1 (MQ=255)

                                        tttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTggg                                                            >  2:223652/1‑139 (MQ=255)

                                          tGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCg                                                          <  1:1135635/139‑1 (MQ=255)

                                            tCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                        <  2:1046095/139‑1 (MQ=255)

                                             cACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCgggg                                                       >  1:1077653/1‑139 (MQ=255)

                                                  cGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTc                                                  <  1:988695/139‑1 (MQ=255)

                                                        gcaACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACt                                            <  1:1211591/139‑1 (MQ=255)

                                                             cTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAg                                       <  1:138525/139‑1 (MQ=255)

                                                               ggAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCt                                                                 >  2:193506/1‑111 (MQ=255)

                                                               ggAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCt                                                                 <  1:193506/111‑1 (MQ=255)

                                                                      ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                        <  1:42324/113‑1 (MQ=255)

                                                                      ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                        >  2:42324/1‑113 (MQ=255)

                                                                      ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtg                              >  2:853612/1‑139 (MQ=255)

                                                                                 cGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                           <  1:1103566/131‑1 (MQ=255)

                                                                                 cGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                           >  2:1103566/1‑131 (MQ=255)

                                                                                  gCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCa                                                                           >  2:34439/1‑82 (MQ=255)

                                                                                  gCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCa                                                                           <  1:34439/82‑1 (MQ=255)

                                                                                  gCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                        >  2:889383/1‑101 (MQ=255)

                                                                                  gCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                        <  1:889383/101‑1 (MQ=255)

                                                                                   cACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                 <  1:247516/139‑1 (MQ=255)

                                                                                        gCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt            <  2:1181297/139‑1 (MQ=255)

                                                                                        gCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt            >  2:288446/1‑139 (MQ=255)

                                                                                             cgcgTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTa          >  1:1012673/1‑136 (MQ=255)

                                                                                             cgcgTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTa          <  2:1012673/136‑1 (MQ=255)

                                                                                               cgTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt     <  1:288446/139‑1 (MQ=255)

                                                                                                  tGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCgg  <  2:1077653/139‑1 (MQ=255)

                                                                                                              cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                        >  2:831365/1‑73 (MQ=255)

                                                                                                              cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                        <  1:831365/73‑1 (MQ=255)

CGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGG  >  NC_000913/3182891‑3183127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A