Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,472,033	G→A	I494I (ATC→ATT)	fusA ←	protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,472,033	0	G	A	98.7%	241.1 / ‑4.3	76	I494I (ATC→ATT)	fusA	protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (39/36); total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

CGTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGGATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGA  >  NC_000913/3471900‑3472158

cGTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGGATAGt                                                                                                                          >  2:109972/1‑139 (MQ=255)

 gTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGtt                                                                                                                         >  1:586846/1‑139 (MQ=255)

 gTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGtt                                                                                                                         >  1:760356/1‑139 (MQ=255)

            cGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGc                                                                                                              >  2:392818/1‑139 (MQ=255)

            cGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGc                                                                                                              >  2:393334/1‑139 (MQ=255)

                  cTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                        <  1:536625/139‑1 (MQ=255)

                    ttCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCg                                                                                                      >  2:1217643/1‑139 (MQ=255)

                    ttCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCg                                                                                                      <  1:393334/139‑1 (MQ=255)

                    ttCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCg                                                                                                      >  1:133574/1‑139 (MQ=255)

                    ttCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCg                                                                                                      <  2:867533/139‑1 (MQ=255)

                    ttCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATAGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCg                                                                                                      <  1:448860/139‑1 (MQ=255)

                           ttGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTAcc                                                                                               >  2:626248/1‑139 (MQ=255)

                           ttGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTAcc                                                                                               >  2:651203/1‑139 (MQ=255)

                            tGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCt                                                                                              <  2:570577/139‑1 (MQ=255)

                                 ccGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGtt                                                                                         <  1:392818/139‑1 (MQ=255)

                                   ggCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                     >  2:329023/1‑109 (MQ=255)

                                   ggCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                     <  1:329023/109‑1 (MQ=255)

                                   ggCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGtt                                                                                         <  1:1047806/137‑1 (MQ=255)

                                   ggCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGtt                                                                                         >  2:1047806/1‑137 (MQ=255)

                                   ggCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCg                                                                                       >  2:1086737/1‑139 (MQ=255)

                                                aCATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                     <  2:435705/96‑1 (MQ=255)

                                                aCATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                     >  1:435705/1‑96 (MQ=255)

                                                   tGTCGATAACAACATGACCATCCTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTc                                                                       <  1:651203/139‑1 (MQ=255)

                                                   tGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTc                                                                       <  1:1086737/139‑1 (MQ=255)

                                                       gATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGc                                                                   >  2:975729/1‑139 (MQ=255)

                                                         tAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                        <  2:788340/100‑1 (MQ=255)

                                                         tAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                        >  1:788340/1‑100 (MQ=255)

                                                            caacaTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCAt                                                              >  2:1201151/1‑139 (MQ=255)

                                                             aacaTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGtt                                                                                         <  2:1184611/111‑1 (MQ=255)

                                                             aacaTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGtt                                                                                         >  1:1184611/1‑111 (MQ=255)

                                                             aacaTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATa                                                             <  1:975729/139‑1 (MQ=255)

                                                              acaTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATAc                                                            <  1:1217643/139‑1 (MQ=255)

                                                               caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                        >  1:653606/1‑94 (MQ=255)

                                                               caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                        <  2:653606/94‑1 (MQ=255)

                                                                       tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAAcc                                                                                                          <  2:372341/84‑1 (MQ=255)

                                                                       tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAAcc                                                                                                          >  1:372341/1‑84 (MQ=255)

                                                                       tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACga                                                   >  2:962574/1‑139 (MQ=255)

                                                                       tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACga                                                   >  2:381032/1‑139 (MQ=255)

                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCt                                                                                     >  2:486699/1‑100 (MQ=255)

                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCt                                                                                     <  1:486699/100‑1 (MQ=255)

                                                                              cacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTc                                            >  2:839305/1‑139 (MQ=255)

                                                                               acgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCg                                           >  1:422484/1‑139 (MQ=255)

                                                                                 gaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAAcc                                                                                                          >  1:221499/1‑74 (MQ=255)

                                                                                 gaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAAcc                                                                                                          <  2:221499/74‑1 (MQ=255)

                                                                                      ccaGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAg                                                                                                               >  1:1131755/1‑64 (MQ=255)

                                                                                      ccaGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAg                                                                                                               <  2:1131755/64‑1 (MQ=255)

                                                                                        aGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGt                                  <  1:345133/139‑1 (MQ=255)

                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGt                                  >  2:1211702/1‑133 (MQ=255)

                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGt                                  <  1:1211702/133‑1 (MQ=255)

                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGccc                            >  1:491045/1‑139 (MQ=255)

                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGccc                            >  1:691267/1‑139 (MQ=255)

                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGccc                            >  1:925381/1‑139 (MQ=255)

                                                                                                 cgcgTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATa                         >  2:1169075/1‑139 (MQ=255)

                                                                                                 cgcgTGTTTACCTTCAACA‑CGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAc                        >  2:831286/1‑139 (MQ=255)

                                                                                                   cgTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                       >  2:295145/1‑139 (MQ=255)

                                                                                                   cgTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                       >  1:699634/1‑139 (MQ=255)

                                                                                                     tgtTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                     <  1:269521/139‑1 (MQ=255)

                                                                                                      gtTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcgc                    >  1:99209/1‑139 (MQ=255)

                                                                                                        ttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGtt                                                                             <  1:153583/80‑1 (MQ=255)

                                                                                                        ttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGtt                                                                             >  2:153583/1‑80 (MQ=255)

                                                                                                         tACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgat                 >  2:1200145/1‑139 (MQ=255)

                                                                                                          aCCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgatg                <  1:962574/139‑1 (MQ=255)

                                                                                                          aCCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgatg                <  2:133574/139‑1 (MQ=255)

                                                                                                          aCCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgatg                <  1:1201151/139‑1 (MQ=255)

                                                                                                             ttCAACA‑CGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTCGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATgg            <  1:831286/139‑1 (MQ=255)

                                                                                                                aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc          <  1:1200145/139‑1 (MQ=255)

                                                                                                                aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc          <  2:699634/139‑1 (MQ=255)

                                                                                                                    tCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGt      <  1:295145/139‑1 (MQ=255)

                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACgatga                                                >  2:663922/1‑95 (MQ=255)

                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACgatga                                                <  1:663922/95‑1 (MQ=255)

                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTa    <  2:422484/139‑1 (MQ=255)

                                                                                                                       gTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTgg       <  1:378542/135‑1 (MQ=255)

                                                                                                                       gTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTgg       >  2:378542/1‑135 (MQ=255)

                                                                                                                        tAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGa  <  2:691267/139‑1 (MQ=255)

                                                                                                                               cTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATgg            <  1:406160/122‑1 (MQ=255)

                                                                                                                               cTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATgg            >  2:406160/1‑122 (MQ=255)

CGTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGGATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGA  >  NC_000913/3471900‑3472158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A