Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,472,033 G→A I494I (ATC→ATT)  fusA ← protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,472,0330GA100.0% 234.7 / NA 72I494I (ATC→ATT) fusAprotein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/38);  total (34/38)

GTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGGATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTC  >  NC_000913/3471901‑3472167
                                                                                                                                    |                                                                                                                                      
gTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGtt                                                                                                                                  >  1:1843145/1‑139 (MQ=255)
gTTGATAAACTCGTAGCCTTTCGGGTTTAAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGtt                                                                                                                                  >  1:491911/1‑139 (MQ=255)
               gCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAAcc                                                                                                                   <  1:1832601/139‑1 (MQ=255)
                 cTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                                 <  2:1358172/139‑1 (MQ=255)
                 cTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                                 <  2:348362/139‑1 (MQ=255)
                 cTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                                 <  1:984964/139‑1 (MQ=255)
                           tGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCt                                                                                                       >  2:1165736/1‑139 (MQ=255)
                                  ggCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCg                                                                                                >  2:467121/1‑139 (MQ=255)
                                   gCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGc                                                                                               >  2:1093039/1‑139 (MQ=255)
                                          cGGGTACATGTCGATAACAACATGACCATACTGACGACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              <  1:1281117/101‑1 (MQ=255)
                                          cGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCgg                                                                                                              >  2:7466/1‑117 (MQ=255)
                                          cGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCgg                                                                                                              <  1:7466/117‑1 (MQ=255)
                                          cGGGTACATGTCGATAACAACACGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCCACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              >  2:1281117/1‑101 (MQ=255)
                                              tACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGa                                                                                    >  1:932213/1‑139 (MQ=255)
                                                 aTGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAAtt                                                                                 <  1:75203/139‑1 (MQ=255)
                                                     cGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACg                                                                             <  1:1453843/139‑1 (MQ=255)
                                                     cGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACg                                                                             <  1:1165736/139‑1 (MQ=255)
                                                      gATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGc                                                                            >  2:537073/1‑139 (MQ=255)
                                                            aacaTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATa                                                                      <  1:624959/139‑1 (MQ=255)
                                                              caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCa                                                                               >  2:90278/1‑128 (MQ=255)
                                                              caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCa                                                                               <  1:90278/128‑1 (MQ=255)
                                                              caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    <  2:491911/139‑1 (MQ=255)
                                                                      tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCAt                                                                       >  1:388420/1‑128 (MQ=255)
                                                                      tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCAt                                                                       <  2:388420/128‑1 (MQ=255)
                                                                        cTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACgatg                                                          <  2:1080670/139‑1 (MQ=255)
                                                                           accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    >  1:1650301/1‑126 (MQ=255)
                                                                           accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    >  1:106990/1‑126 (MQ=255)
                                                                           accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    <  2:106990/126‑1 (MQ=255)
                                                                           accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    <  2:1650301/126‑1 (MQ=255)
                                                                           accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGatgatg                                                       >  1:1067092/1‑139 (MQ=255)
                                                                           accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGatgatg                                                       >  1:933210/1‑139 (MQ=255)
                                                                             cacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTc                                                     >  1:1798478/1‑139 (MQ=255)
                                                                             cacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTc                                                     >  1:1143505/1‑139 (MQ=255)
                                                                              acgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              <  2:214339/65‑1 (MQ=255)
                                                                              acgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              >  1:214339/1‑65 (MQ=255)
                                                                                 accaccAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAgg                                                 <  2:1192877/139‑1 (MQ=255)
                                                                                     ccaGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCa                                             >  2:1765148/1‑139 (MQ=255)
                                                                                             tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTAc                                                                                                         >  1:39938/1‑71 (MQ=255)
                                                                                             tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTAc                                                                                                         <  2:39938/71‑1 (MQ=255)
                                                                                             tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTc                                                                                <  2:1297839/96‑1 (MQ=255)
                                                                                             tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTc                                                                                >  1:1297839/1‑96 (MQ=255)
                                                                                             tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGt                                                <  1:546859/128‑1 (MQ=255)
                                                                                             tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGt                                                >  2:546859/1‑128 (MQ=255)
                                                                                             tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGccc                                     >  1:1357314/1‑139 (MQ=255)
                                                                                                  cgTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACATGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                                <  2:998179/139‑1 (MQ=255)
                                                                                                      tttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGt                                           >  2:1067746/1‑124 (MQ=255)
                                                                                                      tttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGt                                           <  1:1067746/124‑1 (MQ=255)
                                                                                                       ttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                                <  2:1423308/134‑1 (MQ=255)
                                                                                                       ttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                                >  1:1423308/1‑134 (MQ=255)
                                                                                                         aCCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgatg                         <  2:1067092/139‑1 (MQ=255)
                                                                                                         aCCTTCAACATCGGTAACTTTATGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGTgatg                         <  1:1093039/139‑1 (MQ=255)
                                                                                                          ccTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgatga                        <  2:933210/139‑1 (MQ=255)
                                                                                                               aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCGACCTGCGGTTTACCTACGTTCGCTTCGACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc                   <  2:1143505/139‑1 (MQ=255)
                                                                                                               aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              <  2:1305697/128‑1 (MQ=255)
                                                                                                               aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              >  1:1305697/1‑128 (MQ=255)
                                                                                                                    cGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              <  2:284768/123‑1 (MQ=255)
                                                                                                                    cGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              >  1:284768/1‑123 (MQ=255)
                                                                                                                     ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              <  1:1855177/122‑1 (MQ=255)
                                                                                                                     ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              >  2:1855177/1‑122 (MQ=255)
                                                                                                                     ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATgg                     <  1:675594/131‑1 (MQ=255)
                                                                                                                     ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATgg                     >  2:675594/1‑131 (MQ=255)
                                                                                                                     ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTa             >  1:385762/1‑139 (MQ=255)
                                                                                                                          cTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGAttc        <  2:1357314/139‑1 (MQ=255)
                                                                                                                            ttCTGGCGAATAGTTTCACGATAAGAAACCTGCGGATTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTCGTTAGAttctt      >  1:185004/1‑139 (MQ=255)
                                                                                                                             tCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCa                                                                >  2:901308/1‑80 (MQ=255)
                                                                                                                             tCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCa                                                                <  1:901308/80‑1 (MQ=255)
                                                                                                                              cTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCg    <  1:467121/139‑1 (MQ=255)
                                                                                                                              cTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCg    <  1:537073/139‑1 (MQ=255)
                                                                                                                               tGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGt   >  2:1449121/1‑139 (MQ=255)
                                                                                                                                ggCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTc  <  1:569031/139‑1 (MQ=255)
                                                                                                                                 gCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGCATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc                   >  1:553224/1‑121 (MQ=255)
                                                                                                                                 gCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCGTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc                   <  2:553224/121‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGGATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTC  >  NC_000913/3471901‑3472167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A