Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,728,118	(C)_8→9	coding (1254/1455 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,110	1	.	C	100.0%	276.5 / NA	76	D421G (GAT→GGT)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (34/42); total (34/42)

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC  >  NC_000913/3727976‑3728234

gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                          <  1:416993/139‑1 (MQ=255)

           tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccccgtac                                                                                                                 <  1:721825/137‑6 (MQ=255)

           tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccccgtac                                                                                                                 >  2:721825/1‑132 (MQ=255)

                cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                          >  1:741582/1‑123 (MQ=255)

                cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                          <  2:741582/123‑1 (MQ=255)

                cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATc                                                                                                           <  2:669027/139‑1 (MQ=255)

                   cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAg                                                                                                        >  1:332961/1‑139 (MQ=255)

                    gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAgc                                                                                                       <  1:584125/139‑1 (MQ=255)

                    gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAg                                                                                                        <  2:1109795/139‑1 (MQ=255)

                              gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                             <  1:762261/139‑1 (MQ=255)

                                tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                            >  1:308696/1‑139 (MQ=255)

                                tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                           >  1:952786/1‑139 (MQ=255)

                                   gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                        <  2:332961/139‑1 (MQ=255)

                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                   <  1:931474/127‑1 (MQ=255)

                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                   >  2:931474/1‑127 (MQ=255)

                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                       >  2:952453/1‑139 (MQ=255)

                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                        >  2:434959/1‑134 (MQ=255)

                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                        <  1:434959/134‑1 (MQ=255)

                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGCTTTCCGc                                                                                   >  1:527271/1‑139 (MQ=255)

                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                  >  1:973195/1‑139 (MQ=255)

                                           tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                <  2:341778/139‑1 (MQ=255)

                                                gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCacct                                                                           <  2:973195/139‑4 (MQ=255)

                                                gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                           <  2:126495/139‑1 (MQ=255)

                                                gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGACCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                           >  1:332729/1‑139 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         >  1:562242/1‑139 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         <  1:1018916/139‑1 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         >  1:849885/1‑139 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                   <  1:844307/113‑1 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                   >  2:844307/1‑113 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         <  2:989530/139‑1 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         >  1:989530/1‑139 (MQ=255)

                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         >  2:2787/1‑139 (MQ=255)

                                                   gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                        >  1:488483/1‑139 (MQ=255)

                                                    ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGATCTGGCCCCACATC‑CCCCCCCCGTACCGTCATCGAACTTCCGACCGCTGATATCCGCCAACATCTGACg                                                                       >  1:918504/1‑139 (MQ=255)

                                                    ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAATGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                       >  2:758619/1‑139 (MQ=255)

                                                      gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                     <  2:940196/139‑1 (MQ=255)

                                                         gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                  >  1:1134090/1‑139 (MQ=255)

                                                           aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                >  2:322099/1‑139 (MQ=255)

                                                              aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                             <  2:265291/139‑1 (MQ=255)

                                                              aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                             <  1:763315/139‑1 (MQ=255)

                                                                        gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                   <  1:969915/139‑1 (MQ=255)

                                                                        gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                   <  2:488483/139‑1 (MQ=255)

                                                                             ttCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                    <  1:157995/101‑1 (MQ=255)

                                                                             ttCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                    >  2:157995/1‑101 (MQ=255)

                                                                                  tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                         <  1:322099/139‑1 (MQ=255)

                                                                                  tGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                         <  1:758619/139‑1 (MQ=255)

                                                                                  tGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                         <  2:527271/139‑1 (MQ=255)

                                                                                  tGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                         <  1:414240/139‑1 (MQ=255)

                                                                                   ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                         <  2:308696/139‑1 (MQ=255)

                                                                                       tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGTCCCACATC‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                     <  1:595929/74‑1 (MQ=255)

                                                                                       tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGTCCCACATC‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                     >  2:595929/1‑74 (MQ=255)

                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                 >  2:298381/1‑76 (MQ=255)

                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                 <  1:298381/76‑1 (MQ=255)

                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                            <  1:1100607/81‑1 (MQ=255)

                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                            >  2:1100607/1‑81 (MQ=255)

                                                                                         gccgcGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTTAAATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                  >  2:727694/1‑139 (MQ=255)

                                                                                         gccgcGATCTGCACCAGCCTTGCTGCGCCCAGTACTGGCCCCACATC‑CCCCCCCCGTACGGTAATc                                                                                                           <  1:1197195/66‑1 (MQ=255)

                                                                                         gccgcGATCTGCACCAGCCTTGCTGCGCCCAGTACTGGCCCCACATC‑CCCCCCCCGTACGGTAATc                                                                                                           >  2:1197195/1‑66 (MQ=255)

                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                          >  2:743707/1‑49 (MQ=255)

                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                          <  1:743707/49‑1 (MQ=255)

                                                                                          ccgcGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                 <  2:1134090/139‑1 (MQ=255)

                                                                                                 cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                          <  2:562242/139‑1 (MQ=255)

                                                                                                 cTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                          <  1:2787/139‑1 (MQ=255)

                                                                                                   gcaccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAg                                                                                                        <  1:544888/56‑1 (MQ=255)

                                                                                                   gcaccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAg                                                                                                        >  2:544888/4‑59 (MQ=255)

                                                                                                    cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                             <  2:670907/85‑1 (MQ=255)

                                                                                                    cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                             >  1:670907/1‑85 (MQ=255)

                                                                                                     accAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                       >  2:528625/2‑90 (MQ=255)

                                                                                                     accAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                       <  1:528625/89‑1 (MQ=255)

                                                                                                         gCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                  <  2:352186/139‑1 (MQ=255)

                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                      <  2:653952/86‑1 (MQ=255)

                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                      >  1:653952/1‑86 (MQ=255)

                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                  <  2:364752/138‑1 (MQ=255)

                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                  >  1:364752/1‑138 (MQ=255)

                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCCGACCGCTGATATCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                 >  2:440320/1‑139 (MQ=255)

                                                                                                            ttGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGTCCCCAATCAACGTAaca                               >  1:235323/1‑123 (MQ=255)

                                                                                                            ttGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGTCCCCAATCAACGTAaca                               <  2:235323/123‑1 (MQ=255)

                                                                                                                    cccAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         <  2:241887/73‑1 (MQ=255)

                                                                                                                    cccAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                         >  1:241887/1‑73 (MQ=255)

                                                                                                                     ccAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt      <  2:952786/139‑1 (MQ=255)

                                                                                                                         tGCTGGACCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc  <  2:332729/139‑1 (MQ=255)

                                                                                                                              gCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                         >  2:1003866/1‑95 (MQ=255)

                                                                                                                              gCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                         <  1:1003866/95‑1 (MQ=255)

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC  >  NC_000913/3727976‑3728234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A