Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,728,118	(C)_8→9	coding (1254/1455 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,110	1	.	C	96.5%	197.4 / ‑0.9	57	coding (1262/1455 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (24/31); total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA  >  NC_000913/3727972‑3728236

ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑t                                                                                                                                  >  1:1703491/1‑139 (MQ=255)

 gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑c                                                                                                                                >  1:1523799/1‑139 (MQ=255)

    gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccc                                                                                                                             <  1:499951/139‑1 (MQ=255)

               tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACgg                                                                                                                  >  2:589104/1‑139 (MQ=255)

                    cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATc                                                                                                             <  1:215526/139‑1 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTaa                                                                                                               <  2:313088/134‑1 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTaa                                                                                                               <  2:790535/134‑1 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTaa                                                                                                               >  1:790535/1‑134 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTaa                                                                                                               >  1:313088/1‑134 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccccc                                                                                                                            <  1:702910/121‑1 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccccc                                                                                                                            >  2:702910/1‑121 (MQ=255)

                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctg                                                                                                       <  2:746737/139‑1 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctgct                                                                                                     <  1:910090/139‑1 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctgct                                                                                                     >  2:1713981/1‑139 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctgct                                                                                                     <  1:382434/139‑1 (MQ=255)

                               gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGAc                                                                                                  <  2:1523799/139‑1 (MQ=255)

                               gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGAc                                                                                                  <  1:101276/139‑1 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                             >  2:222245/1‑139 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                         <  1:465036/139‑1 (MQ=255)

                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccc                                                                                                                             <  2:1474967/102‑1 (MQ=255)

                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccc                                                                                                                             >  1:1474967/1‑102 (MQ=255)

                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccc                                                                                                                             <  1:383322/99‑1 (MQ=255)

                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccc                                                                                                                             >  2:383322/1‑99 (MQ=255)

                                              cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                   >  2:1782556/1‑139 (MQ=255)

                                              cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAACCGAGCTGCTGACCGCTGATATCCGCCa                                                                                   >  2:846717/1‑139 (MQ=255)

                                               tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                  <  1:200250/139‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctgct                                                                                                     <  1:59892/113‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctgct                                                                                                     >  2:59892/1‑113 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           <  1:1782556/139‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           >  1:805673/1‑139 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           <  1:894407/139‑1 (MQ=255)

                                                         tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                        <  2:1593650/139‑1 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                       <  2:805673/139‑1 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                       <  2:1666328/139‑1 (MQ=255)

                                                            ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccccc                                                                                                                            <  2:116621/84‑1 (MQ=255)

                                                            ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccccc                                                                                                                            >  1:116621/1‑84 (MQ=255)

                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                  >  2:989937/1‑125 (MQ=255)

                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                  <  1:989937/125‑1 (MQ=255)

                                                                acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCTCCGTCCACAAATCGAGCTGCCGACCGCCGAATTCCGTCAGCATCTGACGCCCGTACt                                                                 >  1:1563839/1‑139 (MQ=255)

                                                                           aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccc                                                                                                                             <  2:507432/68‑1 (MQ=255)

                                                                           aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccc                                                                                                                             >  1:507432/1‑68 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                     >  1:1598719/1‑139 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                     >  1:17342/1‑139 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                     <  2:1219901/139‑1 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                     >  1:459146/1‑139 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                     <  2:17342/139‑1 (MQ=255)

                                                                                 ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                <  1:846717/139‑1 (MQ=255)

                                                                                      tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           <  2:245139/139‑1 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACCTa                                    >  2:755387/1‑139 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCCGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                    >  2:74152/1‑139 (MQ=255)

                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCGGACCGCTGGTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                               >  2:3943/1‑139 (MQ=255)

                                                                                                     cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                            <  2:1598719/139‑1 (MQ=255)

                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                            <  1:1529050/136‑1 (MQ=255)

                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                            >  2:1529050/1‑136 (MQ=255)

                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cc                                                                                                                               >  1:1514043/1‑37 (MQ=255)

                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cc                                                                                                                               <  2:1514043/37‑1 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGAc                                                                                                  <  2:1538496/60‑1 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGAc                                                                                                  >  1:1538496/1‑60 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCa                                                                      >  2:1501034/1‑88 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                      <  1:1501034/88‑1 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTaa                                                                                                               >  1:766842/1‑43 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTaa                                                                                                               <  2:766842/43‑1 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCg               >  1:140868/1‑139 (MQ=255)

                                                                                                                     gcgcCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTTATACCGCAg            <  2:1703491/139‑1 (MQ=255)

                                                                                                                       gcCCAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc          <  2:459146/139‑1 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctg                                                                                                       >  2:70590/1‑45 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAgctg                                                                                                       <  1:70590/45‑1 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCCTCTGACGCCCGCACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa         >  2:975144/1‑139 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACA‑TCCCCCCC‑CCGTCCGGTAAACGAGCCGCTGCCCGCTGATATCCGCCAGCATCTGCCGCCAGTACTCACCACGCGCCCCGCCCCCCACCACCGTACCCCTTTGCGGTTTAATACCGCAGGCAtcc      >  2:599962/1‑137 (MQ=255)

                                                                                                                             tGCTGGCCCCACACC‑CCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc    <  2:1563839/139‑1 (MQ=255)

                                                                                                                             tGCTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc    <  2:477034/139‑1 (MQ=255)

                                                                                                                               cTGGCCCCACA‑TCCCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa  <  2:402333/139‑1 (MQ=255)

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCC‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA  >  NC_000913/3727972‑3728236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A