Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,810,304 |
G→A |
*160* (TAG→TAA) |
coaD → |
pantetheine‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,810,304 | 0 | G | A | 100.0%
| 107.5
/ NA
| 34 | *160* (TAG→TAA) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/15); total (19/15) |
TGTGTTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACG > NC_000913/3810169‑3810425
|
tgtgtTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTagcgtaacgt > 2:1167345/1‑139 (MQ=255)
tGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg < 1:1167345/139‑1 (MQ=255)
aTGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa > 1:114525/1‑139 (MQ=255)
gCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATg > 2:183803/1‑139 (MQ=255)
gCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATg > 1:273195/1‑139 (MQ=255)
cGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa < 1:1025676/135‑1 (MQ=255)
cGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa > 2:1025676/1‑135 (MQ=255)
gagTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATc < 2:273195/139‑1 (MQ=255)
tctcTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGaa < 2:114525/139‑1 (MQ=255)
cATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGcc > 1:312461/1‑105 (MQ=255)
cATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGcc < 2:312461/105‑1 (MQ=255)
ttGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGc > 1:58620/1‑139 (MQ=255)
gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAtt > 1:246083/1‑127 (MQ=255)
gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAtt < 2:246083/127‑1 (MQ=255)
aaaGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCAt > 1:122114/1‑108 (MQ=255)
aaaGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCAt < 2:122114/108‑1 (MQ=255)
agagGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGa > 1:502234/1‑139 (MQ=255)
agGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACa > 2:600314/1‑139 (MQ=255)
tGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATg > 1:77125/1‑92 (MQ=255)
tGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATg < 2:77125/92‑1 (MQ=255)
gcgcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAAc > 2:274773/1‑139 (MQ=255)
cGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTgc < 2:459401/139‑1 (MQ=255)
cACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGcc < 2:1023918/65‑1 (MQ=255)
cACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGcc > 1:1023918/1‑65 (MQ=255)
aCCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGtt < 1:274773/139‑1 (MQ=255)
cccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATc > 2:336552/1‑78 (MQ=255)
cccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATc < 1:336552/78‑1 (MQ=255)
ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACaa > 1:1131378/1‑139 (MQ=255)
cGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAAt < 2:58620/139‑1 (MQ=255)
cATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGcc < 2:345182/102‑1 (MQ=255)
cATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGcc > 1:345182/1‑102 (MQ=255)
cAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGcaca > 1:750288/1‑139 (MQ=255)
gcTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGccc > 2:325775/1‑95 (MQ=255)
gcTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGccc < 1:325775/95‑1 (MQ=255)
aTGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCCTACCGCACACCCGACACg > 2:820317/1‑139 (MQ=255)
tagcgtaacgTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAAt < 1:91776/69‑1 (MQ=255)
tagcgtaacgTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAAt > 2:91776/1‑69 (MQ=255)
gtaacgtTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCg < 2:692662/60‑1 (MQ=255)
|
TGTGTTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACG > NC_000913/3810169‑3810425
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
