Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,810,304	G→A	160 (TAG→TAA)	coaD →	pantetheine‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,810,304	0	G	A	96.7%	86.3 / ‑4.8	30	160 (TAG→TAA)	coaD	pantetheine‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (15/14); total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TGTTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACAC  >  NC_000913/3810171‑3810417

tgtTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGttt                                                                                                              >  2:899866/1‑139 (MQ=255)

tgtTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGttt                                                                                                              >  2:1026771/1‑139 (MQ=255)

     cTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGcc                                                                                                         <  1:1026771/139‑1 (MQ=255)

      tGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg                                                                                                        <  1:899866/139‑1 (MQ=255)

        aTGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGTGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa                                                                                                      <  1:970310/139‑1 (MQ=255)

                                               gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCa                                                                 >  1:134027/1‑137 (MQ=255)

                                               gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCa                                                                 <  2:134027/137‑1 (MQ=255)

                                               gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACt                                                               >  1:2261/1‑139 (MQ=255)

                                               gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACt                                                               <  2:2261/139‑1 (MQ=255)

                                                    agagGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTAt                                                                                                >  1:53660/1‑101 (MQ=255)

                                                    agagGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTAt                                                                                                <  2:53660/101‑1 (MQ=255)

                                                          ggCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATaa                                                    >  1:133438/1‑139 (MQ=255)

                                                            cgcgcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGAt                                                                                                     <  2:643215/88‑1 (MQ=255)

                                                            cgcgcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGAt                                                                                                     >  1:643215/1‑88 (MQ=255)

                                                                    cAGGGCGCTGTGACCCATTTCCTGCCGGAGAATGTCCTTGGGGCGCTCGTGGCGAGTGTAGCGTAGAGTGTATGCCGGATGGTATTCGATCCGCGGCGCATGAATTACTTCGTGCACTGGCCAAAATAAAGCGTTGccc                                          <  2:709208/139‑1 (MQ=255)

                                                                      gggCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGc                                        <  2:133438/139‑1 (MQ=255)

                                                                           aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTgcgc                                   >  2:1068433/1‑139 (MQ=255)

                                                                             gTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCgg                                                                                       >  1:816971/1‑85 (MQ=255)

                                                                             gTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCgg                                                                                       <  2:816971/85‑1 (MQ=255)

                                                                                 cccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTg                                             >  2:238643/1‑123 (MQ=255)

                                                                                 cccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTg                                             <  1:238643/123‑1 (MQ=255)

                                                                                    aTTTCCTGCCGGAGAATGTCCATCTGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCGTCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGATGCGCATGTTTAGt                          <  2:618100/139‑1 (MQ=255)

                                                                                               gagaATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCg               >  1:568204/1‑139 (MQ=255)

                                                                                                  aaTGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCa                                                                 >  2:50967/1‑86 (MQ=255)

                                                                                                  aaTGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCa                                                                 <  1:50967/86‑1 (MQ=255)

                                                                                                    tGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGAt                                                                                                     >  1:843443/1‑48 (MQ=255)

                                                                                                    tGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGAt                                                                                                     <  2:843443/48‑1 (MQ=255)

                                                                                                            aGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgc                                                                                    <  1:608563/57‑1 (MQ=255)

                                                                                                            aGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgc                                                                                    >  2:608563/1‑57 (MQ=255)

                                                                                                            aGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCacac  >  2:1078751/1‑139 (MQ=255)

                                                                                                                              tagcgtaacgTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATaaa                                                   >  2:118408/1‑72 (MQ=255)

                                                                                                                              tagcgtaacgTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATaaa                                                   <  1:118408/72‑1 (MQ=255)

TGTTTCTGATGCCGTCGAAAGAGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACAC  >  NC_000913/3810171‑3810417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A