Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,693	G→A	610 (TAG→TAA)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,693	0	G	A	100.0%	153.6 / NA	48	610 (TAG→TAA)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/24); total (24/24)

GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCC  >  NC_000913/4007558‑4007819

gaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATca                                                                                                                             >  1:1014290/1‑139 (MQ=255)

gaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATca                                                                                                                             >  1:765505/1‑139 (MQ=255)

 aTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                            <  1:494714/139‑1 (MQ=255)

   ggCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCACGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagca                                                                                                                          <  1:874442/139‑1 (MQ=255)

     cTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCagcagc                                                                                                                        >  1:837043/1‑139 (MQ=255)

              tGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAg                                                                                                               >  1:348497/1‑139 (MQ=255)

               gCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGt                                                                                                              >  2:621576/1‑139 (MQ=255)

               gCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGt                                                                                                              >  1:920118/1‑139 (MQ=255)

                 cGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCAT‑AAAAAGTGcc                                                                                                           <  2:1014290/139‑1 (MQ=255)

                  gATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           <  2:348497/139‑1 (MQ=255)

                       ccGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTAt                                                                                                      >  2:894352/1‑139 (MQ=255)

                          ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGaa                                                                                                   >  1:1053240/1‑139 (MQ=255)

                            aGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                 <  1:775093/139‑1 (MQ=255)

                            aGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                 <  1:247594/139‑1 (MQ=255)

                              cGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACt                                                                                               <  2:38402/139‑1 (MQ=255)

                              cGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACt                                                                                               >  2:729291/1‑139 (MQ=255)

                                        aGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGt                                                                                     >  2:674992/1‑139 (MQ=255)

                                              aaCGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTcccc                                                                               <  2:837043/139‑1 (MQ=255)

                                                cGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             <  1:361220/139‑1 (MQ=255)

                                                 ggCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTCATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcgc                                                                            <  1:729291/139‑1 (MQ=255)

                                                     ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGt                                                                        <  2:1203681/139‑1 (MQ=255)

                                                       gggATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCa                                                                      <  2:920118/139‑1 (MQ=255)

                                                             cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                 <  2:145197/106‑1 (MQ=255)

                                                             cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                 >  1:145197/1‑106 (MQ=255)

                                                             cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAatgta                                                                >  1:650141/1‑139 (MQ=255)

                                                                aaGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAatgtatgt                                                             <  2:765505/139‑1 (MQ=255)

                                                                 aGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGtt                                                            <  1:546212/139‑1 (MQ=255)

                                                                       aaCGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcgc                                                                            <  1:493124/117‑1 (MQ=255)

                                                                       aaCGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcgc                                                                            >  2:493124/1‑117 (MQ=255)

                                                                            tttGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTtc                                                 >  2:255066/1‑139 (MQ=255)

                                                                                    aCCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTc                                         >  2:948728/1‑139 (MQ=255)

                                                                                               cgcTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGca                              >  2:1034136/1‑139 (MQ=255)

                                                                                               cgcTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGca                              <  2:308103/139‑1 (MQ=255)

                                                                                                 cTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           <  1:61134/60‑1 (MQ=255)

                                                                                                 cTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           >  2:61134/1‑60 (MQ=255)

                                                                                                 cTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTtctc                                               <  2:112579/120‑1 (MQ=255)

                                                                                                 cTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTtctc                                               >  1:112579/1‑120 (MQ=255)

                                                                                                    aTTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATaaaa                                                                                                                  <  1:1028152/50‑1 (MQ=255)

                                                                                                    aTTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATaaaa                                                                                                                  >  2:1028152/1‑50 (MQ=255)

                                                                                                      tCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATtgtg                       <  1:674992/139‑1 (MQ=255)

                                                                                                         tGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGtt                                                            <  2:732129/99‑1 (MQ=255)

                                                                                                         tGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGtt                                                            >  1:732129/1‑99 (MQ=255)

                                                                                                                gTTGATGGCGATGACGAAGAATAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGa             >  1:716307/1‑139 (MQ=255)

                                                                                                                        cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCg                                        >  2:532915/1‑104 (MQ=255)

                                                                                                                        cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCg                                        <  1:532915/104‑1 (MQ=255)

                                                                                                                        cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGt     >  1:756825/1‑139 (MQ=255)

                                                                                                                          aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTcc   >  2:670246/1‑139 (MQ=255)

                                                                                                                           tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTccc  <  2:952608/139‑1 (MQ=255)

GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCC  >  NC_000913/4007558‑4007819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A