Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,693	G→A	610 (TAG→TAA)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,693	0	G	A	100.0%	129.7 / NA	41	610 (TAG→TAA)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/21); total (20/21)

GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCCGATTTCTT  >  NC_000913/4007558‑4007827

gaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGACGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATca                                                                                                                                      >  2:1262916/1‑139 (MQ=255)

             aTGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATaaaaaa                                                                                                                         >  1:232706/1‑139 (MQ=255)

               gCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGt                                                                                                                       >  1:21698/1‑139 (MQ=255)

                ccGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTg                                                                                                                      <  1:1114750/139‑1 (MQ=255)

                ccGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTg                                                                                                                      <  1:2480/139‑1 (MQ=255)

                  gATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATACGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                                    >  2:176115/1‑139 (MQ=255)

                        cGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                                    <  2:993643/133‑1 (MQ=255)

                        cGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                                    >  1:993643/1‑133 (MQ=255)

                          ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                                     >  1:1090036/1‑114 (MQ=255)

                          ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                                     <  2:1090036/114‑1 (MQ=255)

                            aGCGAAATGCTCAGCGTTAACGGCGTTGGGATACGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                          <  1:176115/139‑1 (MQ=255)

                                         gCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGtt                                                                                            <  2:21698/139‑1 (MQ=255)

                                                    gTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCAGGTAT‑AAGAATCCCGTAAAGGtctgtccttattc                                                                                 >  1:114538/1‑127 (MQ=255)

                                                     ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGt                                                                                >  1:343271/1‑139 (MQ=255)

                                                     ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGt                                                                                <  2:232706/139‑1 (MQ=255)

                                                     ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGt                                                                                <  1:876338/139‑1 (MQ=255)

                                                        ggATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCaa                                                                             <  1:202899/139‑1 (MQ=255)

                                                             cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                          >  2:1315638/1‑106 (MQ=255)

                                                             cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                          <  1:1315638/106‑1 (MQ=255)

                                                              gcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAatgtat                                                                       <  2:1225301/139‑1 (MQ=255)

                                                                  gCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAg                                                                                                                        >  2:388761/1‑87 (MQ=255)

                                                                  gCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAg                                                                                                                        <  1:388761/87‑1 (MQ=255)

                                                                               ggCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCa                                                      <  2:343271/139‑1 (MQ=255)

                                                                                      cGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGAc                                                                                                         <  1:436240/82‑1 (MQ=255)

                                                                                      cGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGAc                                                                                                         >  2:436240/1‑82 (MQ=255)

                                                                                            tGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGt                                         <  1:618388/139‑1 (MQ=255)

                                                                                            tGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGt                                         <  1:1262916/139‑1 (MQ=255)

                                                                                                  tGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                          <  2:194885/69‑1 (MQ=255)

                                                                                                  tGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                          >  1:194885/1‑69 (MQ=255)

                                                                                                  tGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAat                                                                           <  2:1006239/99‑1 (MQ=255)

                                                                                                  tGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAat                                                                           >  1:1006239/1‑99 (MQ=255)

                                                                                                              aTGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTAt                       >  2:582309/1‑139 (MQ=255)

                                                                                                                tttGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGa                     >  1:828136/2‑139 (MQ=255)

                                                                                                                          aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCcgc                                                                                    <  2:1310088/66‑1 (MQ=255)

                                                                                                                          aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCcgc                                                                                    >  1:1310088/1‑66 (MQ=255)

                                                                                                                           tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCAc                                                     <  1:1188135/96‑1 (MQ=255)

                                                                                                                           tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCAc                                                     >  2:1188135/1‑96 (MQ=255)

                                                                                                                            gACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCCg         >  2:380719/1‑139 (MQ=255)

                                                                                                                             aCGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGt                                                                                <  2:233826/67‑1 (MQ=255)

                                                                                                                             aCGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGt                                                                                >  1:233826/1‑67 (MQ=255)

                                                                                                                                 agagTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCCGATTTc    >  2:715656/1‑139 (MQ=255)

                                                                                                                                   agtaatCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCTCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCCGATTTCtt  <  1:715656/139‑1 (MQ=255)

GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACT‑CCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCCGATTTCTT  >  NC_000913/4007558‑4007827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A