Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,693	G→A	610 (TAG→TAA)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,693	0	G	A	100.0%	182.3 / NA	57	610 (TAG→TAA)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/31); total (26/31)

GCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCC  >  NC_000913/4007562‑4007819

gCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagcag                                                                                                                         >  1:808280/1‑139 (MQ=255)

   gAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCAt                                                                                                                      <  2:808280/139‑1 (MQ=255)

    aaCAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                            >  2:134593/1‑132 (MQ=255)

    aaCAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                            <  1:134593/132‑1 (MQ=255)

    aaCAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                            <  2:167138/132‑1 (MQ=255)

    aaCAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                            >  1:167138/1‑132 (MQ=255)

          tGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAg                                                                                                               >  1:838953/1‑139 (MQ=255)

              gATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           >  2:865356/1‑139 (MQ=255)

              gATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           <  2:795798/139‑1 (MQ=255)

              gATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           <  2:624073/139‑1 (MQ=255)

                tCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAg                                                                                                         >  2:807979/1‑139 (MQ=255)

                   ccGCCAGCGAAATGCTCAGCGTTACCGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAg                                                                                                         >  1:1094524/1‑136 (MQ=255)

                   ccGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           <  2:298775/134‑1 (MQ=255)

                   ccGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           >  1:298775/1‑134 (MQ=255)

                   ccGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTGTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAg                                                                                                         <  2:1094524/136‑1 (MQ=255)

                              aTGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGt                                                                                           >  1:794180/1‑139 (MQ=255)

                              aTGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGt                                                                                           >  2:477994/1‑139 (MQ=255)

                                 cTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTaaa                                                                                        <  1:807979/139‑1 (MQ=255)

                                   cAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                          >  1:152232/1‑135 (MQ=255)

                                   cAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                          <  2:152232/135‑1 (MQ=255)

                                    aGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGt                                                                                     >  2:676465/1‑139 (MQ=255)

                                            cGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             <  1:1145724/139‑1 (MQ=255)

                                            cGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             <  1:1035451/139‑1 (MQ=255)

                                                 ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGt                                                                        <  1:477994/139‑1 (MQ=255)

                                                    ggATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCaa                                                                     <  1:1124674/139‑1 (MQ=255)

                                                       tGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAatg                                                                  <  1:1200448/139‑1 (MQ=255)

                                                         cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             >  2:1061168/1‑126 (MQ=255)

                                                         cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             <  1:1061168/126‑1 (MQ=255)

                                                           cAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAatgtatg                                                              >  1:483703/1‑139 (MQ=255)

                                                             aGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGtt                                                            <  2:1195127/139‑1 (MQ=255)

                                                                 ggAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATg                                                        >  2:996945/1‑139 (MQ=255)

                                                                        tttGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTtc                                                 >  1:1201938/1‑139 (MQ=255)

                                                                            gCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCAc                                             <  1:409401/139‑1 (MQ=255)

                                                                                   gTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGcc                                      <  1:377202/139‑1 (MQ=255)

                                                                                   gTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGcc                                      <  1:865356/139‑1 (MQ=255)

                                                                                   gTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGcc                                      <  1:454821/139‑1 (MQ=255)

                                                                                    tttATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCa                                     <  1:404617/139‑1 (MQ=255)

                                                                                        tGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGt                                 <  1:676465/139‑1 (MQ=255)

                                                                                         ggCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTg                                <  1:996945/139‑1 (MQ=255)

                                                                                           cgcTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGca                              >  1:639998/1‑139 (MQ=255)

                                                                                                  tCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCATCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATtgtg                       <  2:838953/139‑1 (MQ=255)

                                                                                                     tGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           >  1:564253/1‑52 (MQ=255)

                                                                                                     tGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                           <  2:564253/52‑1 (MQ=255)

                                                                                                       cgcATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCg                                        >  2:120389/1‑117 (MQ=255)

                                                                                                       cgcATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCg                                        <  1:120389/117‑1 (MQ=255)

                                                                                                               gATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGc                                                                                                            <  1:1257901/41‑1 (MQ=255)

                                                                                                               gATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGc                                                                                                            >  2:1257901/1‑41 (MQ=255)

                                                                                                                    cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                 >  2:418074/1‑47 (MQ=255)

                                                                                                                    cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                                 <  1:418074/47‑1 (MQ=255)

                                                                                                                    cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGt     >  1:177574/1‑139 (MQ=255)

                                                                                                                      aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             <  2:466810/65‑1 (MQ=255)

                                                                                                                      aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             <  1:775176/65‑1 (MQ=255)

                                                                                                                      aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             >  2:775176/1‑65 (MQ=255)

                                                                                                                      aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                             >  1:466810/1‑65 (MQ=255)

                                                                                                                       tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGtt                                                      >  2:297348/1‑87 (MQ=255)

                                                                                                                       tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGtt                                                      <  1:297348/87‑1 (MQ=255)

                                                                                                                       tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTccc  >  2:881661/1‑139 (MQ=255)

GCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCC  >  NC_000913/4007562‑4007819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A