Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,997,689 |
C→T |
intergenic (‑89/+3) |
insC1 ← / ← ygeQ |
IS2 repressor TnpA/pseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,997,689 | 0 | C | T | 100.0%
| 149.9
/ NA
| 47 | intergenic (‑89/+3) | insC1/ygeQ | IS2 repressor TnpA/pseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/20); total (27/20) |
AAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATC > NC_000913/2997587‑2997820
|
aaGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttcttc < 2:414127/122‑1 (MQ=255)
aaGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttcttc > 1:414127/1‑122 (MQ=255)
caatcaTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg < 1:976063/139‑1 (MQ=255)
caatcaTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg > 2:976063/1‑139 (MQ=255)
ctcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg < 2:800086/131‑1 (MQ=255)
ctcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg > 1:800086/1‑131 (MQ=255)
tcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAAc > 1:620207/1‑139 (MQ=255)
aCTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGt > 1:324261/1‑139 (MQ=255)
tcgaaaaaCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGc > 2:623646/4‑139 (MQ=255)
aGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg < 2:958248/104‑1 (MQ=255)
aGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg > 1:958248/1‑104 (MQ=255)
aGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCt > 1:92719/1‑139 (MQ=255)
gTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCtt < 2:620207/139‑1 (MQ=255)
ttAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTtctc > 2:471999/1‑139 (MQ=255)
aCTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTaa > 2:84616/1‑139 (MQ=255)
tATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaat > 2:831509/1‑103 (MQ=255)
tATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaat < 1:831509/103‑1 (MQ=255)
aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttctt < 1:626007/67‑1 (MQ=255)
aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttctt > 2:626007/1‑67 (MQ=255)
tCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGtt > 2:121667/1‑139 (MQ=255)
aTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGAttt < 1:90569/84‑1 (MQ=255)
aTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGAttt > 2:90569/1‑84 (MQ=255)
aTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCtt > 1:308297/1‑139 (MQ=255)
aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTg > 2:200525/1‑59 (MQ=255)
aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTg < 1:200525/59‑1 (MQ=255)
gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa < 2:964195/86‑1 (MQ=255)
gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa > 1:964195/1‑86 (MQ=255)
atTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTAt < 2:245436/60‑1 (MQ=255)
atTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTAt > 1:245436/1‑60 (MQ=255)
gTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttc > 1:729929/1‑43 (MQ=255)
gTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttc < 2:729929/43‑1 (MQ=255)
gTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTg > 2:639333/1‑139 (MQ=255)
tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGgtgt < 1:790029/48‑1 (MQ=255)
tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGgtgt > 2:790029/1‑48 (MQ=255)
tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataga < 1:776491/75‑3 (MQ=255)
tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa > 2:776491/1‑75 (MQ=255)
tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATTACATTAAGTAAAGCCGCATCGGCTtct < 2:641556/104‑1 (MQ=255)
tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTtct > 1:641556/1‑104 (MQ=255)
tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGa > 1:945697/1‑139 (MQ=255)
agaTTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATtctc > 2:693346/1‑139 (MQ=255)
cAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg > 2:421456/1‑60 (MQ=255)
cAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg < 1:421456/60‑1 (MQ=255)
cAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCt < 1:639333/139‑1 (MQ=255)
ggCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATc < 1:121667/139‑1 (MQ=255)
ggCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATc < 1:84616/139‑1 (MQ=255)
ccAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg > 2:590692/1‑49 (MQ=255)
ccAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg < 1:590692/49‑1 (MQ=255)
|
AAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATC > NC_000913/2997587‑2997820
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
