Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,176,721 G→A T38I (ACC→ATC)  cpdA ← 3',5' cAMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,176,7210GA97.4% 240.4 / ‑1.3 76coding (113/828 nt)cpdA3',5' cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (37/37);  minor base . (1/1);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

CTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCA  >  NC_000913/3176586‑3176852
                                                                                                                                       |                                                                                                                                   
cTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                  <  1:1363902/139‑1 (MQ=255)
  tGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGttt                                                                                                                                <  2:55521/139‑1 (MQ=255)
     gATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                             <  1:1088865/139‑1 (MQ=255)
     gATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                             <  1:995381/139‑1 (MQ=255)
              aGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAgg                                                                                                                    >  2:1017701/1‑139 (MQ=255)
                   aaTGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTc                                                                                                               >  1:1744887/1‑139 (MQ=255)
                   aaTGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTc                                                                                                               >  1:1849574/1‑139 (MQ=255)
                   aaTGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTc                                                                                                               >  1:484265/1‑139 (MQ=255)
                     tGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGt                                                                                                             >  1:1208574/1‑139 (MQ=255)
                        tGATAGGCCGCAGAGGATTGATCCTGCACTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCt                                                                                                          <  1:1726554/139‑1 (MQ=255)
                            aGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtg                                                                                                      >  2:1635696/1‑139 (MQ=255)
                             ggCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                     <  1:1635696/139‑1 (MQ=255)
                              gCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTGCCCCTAACAGGGCTTCGTGCTTTtgtg                                                                                                    >  1:1072911/1‑139 (MQ=255)
                               ccGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGc                                                                                                   >  1:1213029/1‑139 (MQ=255)
                                cGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                  <  1:1192104/139‑1 (MQ=255)
                                 gCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTaa                                                                                                                        <  1:324745/116‑1 (MQ=255)
                                 gCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTaa                                                                                                                        >  2:324745/1‑116 (MQ=255)
                                      agacagATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACACTCCCAGATGTTTACCCCTAACAGAGCTTCGTGCTTTTGTGCAAACAgg                                                                                            <  2:975773/134‑1 (MQ=255)
                                       gATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                           <  2:1213029/139‑1 (MQ=255)
                                            aTCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAgtg                                                                                      >  2:411761/1‑139 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                                 <  2:847453/91‑1 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                                 >  1:847453/1‑91 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcccc                                                                                                                           >  2:1641020/1‑97 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcccc                                                                                                                           <  1:1641020/97‑1 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCg                                                                                   >  1:732569/1‑137 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCg                                                                                   <  2:732569/137‑1 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                 >  1:1250508/1‑139 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCTCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGTGCTTCGTGCTTGTGTGCAAACACGTGAGTGTCGGtctgtct                                                                           >  1:975773/1‑133 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCt                                                                                                                 <  1:1314310/101‑1 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCt                                                                                                                 >  2:1314310/1‑101 (MQ=255)
                                                        aTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCGCCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGt                                                                          <  1:1121936/139‑1 (MQ=255)
                                                          cACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTaa                                                                        <  1:411761/139‑1 (MQ=255)
                                                            ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCtt                                                                                                                <  2:1146980/97‑1 (MQ=255)
                                                            ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCtt                                                                                                                >  1:1146980/1‑97 (MQ=255)
                                                            ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTaaaa                                                                      >  2:1821224/1‑139 (MQ=255)
                                                                 cgcgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTg                                                                 >  2:1816666/1‑139 (MQ=255)
                                                                 cgcgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTg                                                                 >  1:84952/1‑139 (MQ=255)
                                                                 cgcgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTg                                                                 >  2:789510/1‑139 (MQ=255)
                                                                    gACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCTTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGAct                                                              <  1:565141/139‑1 (MQ=255)
                                                                       aaTCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCA‑‑‑GTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTgg                                                          <  1:1780878/137‑1 (MQ=255)
                                                                       aaTCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCA‑‑‑GTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTgg                                                          >  2:1780878/1‑137 (MQ=255)
                                                                             gTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCa                                                     <  1:1816666/139‑1 (MQ=255)
                                                                                       tGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATcc                                                                   >  2:1784155/1‑115 (MQ=255)
                                                                                       tGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATcc                                                                   <  1:1784155/115‑1 (MQ=255)
                                                                                       tGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCagag                                           <  1:1017701/139‑1 (MQ=255)
                                                                                        gCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAgg                                          <  2:84952/139‑1 (MQ=255)
                                                                                           ggTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaaa                                                                                                >  1:690920/1‑82 (MQ=255)
                                                                                           ggTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaaa                                                                                                <  2:690920/82‑1 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTTCCAGATGTTTACCCCTAACATGGCTTCGTGCTTTTGTGCAAACAGGTGa                                                                                         <  1:1218054/83‑1 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAg                                                                                                                     <  1:683332/55‑1 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAg                                                                                                                     >  2:683332/1‑55 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCttt                                                                                                        >  1:900886/1‑68 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCttt                                                                                                        <  2:900886/68‑1 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGa                                                                                         >  2:1218054/1‑83 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTgg                                                          >  2:1672171/1‑114 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTgg                                                          <  1:1672171/114‑1 (MQ=255)
                                                                                                      aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGGAGGGTTAACAGGc                            >  2:789193/1‑139 (MQ=255)
                                                                                                      aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                            <  1:1821224/139‑1 (MQ=255)
                                                                                                                    cGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCt                                                                                                          <  2:1740234/47‑1 (MQ=255)
                                                                                                                    cGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCt                                                                                                          >  1:1740234/1‑47 (MQ=255)
                                                                                                                      ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTcc            >  1:1406477/1‑139 (MQ=255)
                                                                                                                        tGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGGAGGGTTAACAGGCTTTCCAAATGGTGTCCtt          <  1:789193/139‑1 (MQ=255)
                                                                                                                        tGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt          <  2:484265/139‑1 (MQ=255)
                                                                                                                        tGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt          <  2:180402/139‑1 (MQ=255)
                                                                                                                         ggTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATg                  >  1:1446689/1‑130 (MQ=255)
                                                                                                                         ggTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATg                  <  2:1446689/130‑1 (MQ=255)
                                                                                                                           tAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAgg       >  1:1563427/1‑139 (MQ=255)
                                                                                                                              ctctCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGttt    >  2:322178/1‑139 (MQ=255)
                                                                                                                                ctcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCa  <  2:1208574/139‑1 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAgg                                                                                            <  1:1560711/48‑1 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAgg                                                                                            >  2:1560711/1‑48 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTaaaa                                                                      >  1:486208/1‑70 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTaaaa                                                                      <  2:486208/70‑1 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCagag                                           >  2:1647048/1‑97 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCagag                                           <  1:1647048/97‑1 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATg                  >  1:1509007/1‑122 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATg                  <  2:1509007/122‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCA  >  NC_000913/3176586‑3176852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A