Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,176,721	G→A	T38I (ACC→ATC)	cpdA ←	3',5' cAMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,176,721	0	G	A	100.0%	144.7 / NA	45	T38I (ACC→ATC)	cpdA	3',5' cAMP phosphodiesterase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/24); total (21/24)

TTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGT  >  NC_000913/3176587‑3176838

ttGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                   <  2:249460/139‑1 (MQ=255)

    gATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                               <  2:388633/139‑1 (MQ=255)

      tGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcccc                                                                                                             <  1:530846/139‑1 (MQ=255)

                    tGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGt                                                                                               >  1:588945/1‑139 (MQ=255)

                               cGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCtt                                                                                           >  2:907403/1‑132 (MQ=255)

                               cGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCtt                                                                                           <  1:907403/132‑1 (MQ=255)

                                 cAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaaa                                                                                  >  2:45120/1‑139 (MQ=255)

                                 cAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCACCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaaa                                                                                  <  2:631269/139‑1 (MQ=255)

                                    agGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAg                                                                               <  2:678699/139‑1 (MQ=255)

                                      gATTGATCCTGCGCTAAATCACCTGTCGCTACAATCAGGTCGAATGCGTGCTAGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                   >  1:583441/1‑101 (MQ=255)

                                      gATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGtt                                                                                                                   <  2:583441/101‑1 (MQ=255)

                                       aTTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTg                                                                            <  2:534542/139‑1 (MQ=255)

                                       aTTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTg                                                                            <  1:45120/139‑1 (MQ=255)

                                           aTCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTg                                                                            <  2:73260/135‑1 (MQ=255)

                                           aTCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTg                                                                            >  1:73260/1‑135 (MQ=255)

                                              cTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCg                                                                     >  1:880653/1‑139 (MQ=255)

                                              cTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCg                                                                     >  2:910206/1‑139 (MQ=255)

                                                gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                               <  2:904469/95‑1 (MQ=255)

                                                gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                               >  1:904469/1‑95 (MQ=255)

                                                 cgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCt                                                                                                   <  2:606721/106‑1 (MQ=255)

                                                 cgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTa                                                                  >  1:984514/1‑139 (MQ=255)

                                                 cgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTa                                                                  >  2:276643/1‑139 (MQ=255)

                                                 cacTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTCTACCCCTGACAGGGCt                                                                                                   >  1:606721/3‑106 (MQ=255)

                                                      aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTg                                                             >  1:5350/1‑139 (MQ=255)

                                                              gTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAGCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGa                                                                           >  1:612863/1‑117 (MQ=255)

                                                              gTCGCGACAATCAGGTCCAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGa                                                                           <  2:612863/117‑1 (MQ=255)

                                                                cgcgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTg                                                   >  1:473708/1‑139 (MQ=255)

                                                                        tCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGc                                           >  2:239677/1‑139 (MQ=255)

                                                                            gTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCATCa                                       <  1:219853/139‑1 (MQ=255)

                                                                                 aTTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                  <  2:473708/139‑1 (MQ=255)

                                                                                      tGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                  >  2:44692/1‑134 (MQ=255)

                                                                                      tGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                  <  1:44692/134‑1 (MQ=255)

                                                                                       gCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAgg                            >  1:889603/1‑139 (MQ=255)

                                                                                          ggTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAg                         <  2:889603/139‑1 (MQ=255)

                                                                                           gtgtGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCtt                                                                                           <  2:125613/72‑1 (MQ=255)

                                                                                           gtgtGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCtt                                                                                           >  1:125613/1‑72 (MQ=255)

                                                                                                gCCGAATCGCCTCCAGCCCCGCCTGGTAACTCTCCCAGatgt                                                                                                                    >  2:631522/1‑42 (MQ=38)

                                                                                                gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgc                                                                                                                    <  1:631522/42‑2 (MQ=255)

                                                                                                gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATcc                                                     >  1:818438/1‑105 (MQ=255)

                                                                                                gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATcc                                                     <  2:818438/105‑1 (MQ=255)

                                                                                                  cGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACa                 <  2:526210/139‑1 (MQ=255)

                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc              <  2:984514/139‑1 (MQ=255)

                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc              <  2:588945/139‑1 (MQ=255)

                                                                                                     aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc              >  1:641240/1‑139 (MQ=255)

                                                                                                                 aCCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGgt  <  2:880653/139‑1 (MQ=255)

                                                                                                                     ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCt                                         >  2:136635/1‑96 (MQ=255)

                                                                                                                     ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCt                                         <  1:136635/96‑1 (MQ=255)

TTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGT  >  NC_000913/3176587‑3176838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A