Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,767,179	G→A	281 (TAG→TAA)	yibA →	putative immunity protein for polymorphic toxin RhsA, HEAT‑domain protein, lethality reduction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,767,179	0	G	A	100.0%	210.0 / NA	65	281 (TAG→TAA)	yibA	putative immunity protein for polymorphic toxin RhsA, HEAT‑domain protein, lethality reduction protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/32); total (33/32)

TATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3767048‑3767311

gatgatgATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTACCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               <  1:1471413/138‑1 (MQ=255)

   gatgatATCATTGAAGCTGCGGGAGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               <  2:249923/136‑1 (MQ=255)

   gatgatATCATTGAAGCTGCGGGAGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               >  1:249923/1‑136 (MQ=255)

        tatCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATg                                                                                                                       >  1:549103/1‑139 (MQ=255)

        tatCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGCGAGTTGCCTATg                                                                                                                       >  1:1157366/1‑139 (MQ=255)

        tatCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCATGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATg                                                                                                                       >  2:1087039/1‑139 (MQ=255)

          tCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGca                                                                                                                     <  1:1087039/139‑1 (MQ=255)

            aTTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGcaca                                                                                                                   >  1:342056/1‑139 (MQ=255)

             ttGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAg                                                                                                                  <  1:1429846/139‑1 (MQ=255)

                              ttAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                 <  2:549103/139‑1 (MQ=255)

                                aGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               <  1:966293/139‑1 (MQ=255)

                                 ggTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGCGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCAc                                                                                              <  2:1157366/139‑1 (MQ=255)

                                  gTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACg                                                                                             >  1:52759/1‑139 (MQ=255)

                                   tGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTcc                                                                                                       >  2:1318542/1‑128 (MQ=255)

                                   tGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTcc                                                                                                       <  1:1318542/128‑1 (MQ=255)

                                      tAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCt                                                                                                                          >  2:909822/1‑106 (MQ=255)

                                      tAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCt                                                                                                                          <  1:909822/106‑1 (MQ=255)

                                        aaaCGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAg                                                                                       >  2:77817/1‑139 (MQ=255)

                                           cGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCa                                                                                        >  2:1239700/1‑135 (MQ=255)

                                           cGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCa                                                                                        <  1:1239700/135‑1 (MQ=255)

                                               aCTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCAGGCACGGTGCAGATGCTCg                                                                                >  1:982672/1‑139 (MQ=255)

                                                          tAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               >  2:1317801/1‑113 (MQ=255)

                                                          tAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               <  1:1317801/113‑1 (MQ=255)

                                                                 tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                 <  1:289899/104‑1 (MQ=255)

                                                                 tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                 >  2:289899/1‑104 (MQ=255)

                                                                 tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               <  1:379176/106‑1 (MQ=255)

                                                                 tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               >  2:379176/1‑106 (MQ=255)

                                                                 tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGaaa                                                              <  1:304224/139‑1 (MQ=255)

                                                                 tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGaaa                                                              >  2:295875/1‑139 (MQ=255)

                                                                   tgttgtACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               >  1:569614/1‑72 (MQ=255)

                                                                   tgttgtACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               <  2:569614/72‑1 (MQ=255)

                                                                      tgtACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGc                                                                                                                      >  2:1248523/1‑78 (MQ=255)

                                                                      tgtACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGc                                                                                                                      <  1:1248523/78‑1 (MQ=255)

                                                                             gTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGAcc                                                  >  1:577266/1‑139 (MQ=255)

                                                                             gTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGAcc                                                  >  1:1493549/1‑139 (MQ=255)

                                                                               ttGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                <  1:77817/139‑1 (MQ=255)

                                                                                  atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAg                                                                                                                  <  2:1095881/70‑1 (MQ=255)

                                                                                  atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAg                                                                                                                  >  1:1095881/1‑70 (MQ=255)

                                                                                  atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                             >  1:992098/1‑139 (MQ=255)

                                                                                    gaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgag                                           <  2:342056/139‑1 (MQ=255)

                                                                                       aaTGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTgg                                                        >  1:1380136/1‑123 (MQ=255)

                                                                                       aaTGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTgg                                                        <  2:1380136/123‑1 (MQ=255)

                                                                                         tGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGACTGGAAAGTGAAATCCTGGCTGACCGGGTGACTGAgg                                      >  2:377708/1‑139 (MQ=255)

                                                                                                 taACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgagtg                                         <  2:968822/128‑1 (MQ=255)

                                                                                                 taACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgagtg                                         >  1:968822/1‑128 (MQ=255)

                                                                                                   aCTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                            <  2:52759/139‑1 (MQ=255)

                                                                                                   aCTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                            <  1:295875/139‑1 (MQ=255)

                                                                                                       ccGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACATTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgc                                 <  2:208046/130‑1 (MQ=255)

                                                                                                       ccGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgc                                 >  1:208046/1‑130 (MQ=255)

                                                                                                          cTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtga                                            <  1:1069238/116‑1 (MQ=255)

                                                                                                          cTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtga                                            >  2:1069238/1‑116 (MQ=255)

                                                                                                                    gCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCg           >  2:184187/1‑139 (MQ=255)

                                                                                                                       gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               <  2:1018871/52‑1 (MQ=255)

                                                                                                                       gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               >  1:827093/1‑52 (MQ=255)

                                                                                                                       gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               <  2:827093/52‑1 (MQ=255)

                                                                                                                       gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               >  1:1018871/1‑52 (MQ=255)

                                                                                                                       gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                 <  2:1430980/82‑1 (MQ=255)

                                                                                                                       gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                 >  1:1430980/1‑82 (MQ=255)

                                                                                                                       gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGcc        <  1:184187/139‑1 (MQ=255)

                                                                                                                         aGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCg                                                 >  2:1092978/1‑96 (MQ=255)

                                                                                                                         aGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCg                                                 <  1:1092978/96‑1 (MQ=255)

                                                                                                                         aGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGACTGGCATCGTGCGGCCTg      >  1:784781/1‑139 (MQ=255)

                                                                                                                             ttCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCg  <  2:992098/139‑1 (MQ=255)

                                                                                                                              tCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCggg                                               <  1:981371/93‑1 (MQ=255)

                                                                                                                              tCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCggg                                               >  2:981371/1‑93 (MQ=255)

TATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3767048‑3767311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A