Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,767,179	G→A	281 (TAG→TAA)	yibA →	putative immunity protein for polymorphic toxin RhsA, HEAT‑domain protein, lethality reduction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,767,179	0	G	A	100.0%	191.0 / NA	59	281 (TAG→TAA)	yibA	putative immunity protein for polymorphic toxin RhsA, HEAT‑domain protein, lethality reduction protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/27); total (32/27)

TATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3767056‑3767311

tatCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACTTGAGt                                                                                                                               <  1:887942/131‑1 (MQ=255)

tatCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               >  2:887942/1‑131 (MQ=255)

tatCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATg                                                                                                                       <  2:1288722/139‑1 (MQ=255)

                           tGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACgg                                                                                            >  2:1205941/1‑139 (MQ=255)

                              tAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGc                                                                                         >  1:1295355/1‑139 (MQ=255)

                              tAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGc                                                                                         >  2:1447988/1‑139 (MQ=255)

                                   cGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               >  1:255511/1‑128 (MQ=255)

                                   cGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               <  2:255511/128‑1 (MQ=255)

                                                 ttAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGa                                                                      <  2:1295355/139‑1 (MQ=255)

                                                         tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGaaa                                                              >  2:1648846/1‑139 (MQ=255)

                                                                tACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGa                                                                                                                                 <  2:663706/65‑1 (MQ=255)

                                                                tACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGa                                                                                                                                 >  1:663706/1‑65 (MQ=255)

                                                                     gTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGa                                                                                      <  1:1535177/103‑1 (MQ=255)

                                                                     gTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGa                                                                                      >  2:1535177/1‑103 (MQ=255)

                                                                          atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                             >  2:1456964/1‑139 (MQ=255)

                                                                          atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                             >  1:1274461/1‑139 (MQ=255)

                                                                           tgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtga                                            <  2:1274461/139‑1 (MQ=255)

                                                                               aaTGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgagtga                                        >  2:343620/1‑139 (MQ=255)

                                                                               aaTGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgagtga                                        >  2:597569/1‑139 (MQ=255)

                                                                                aTGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGc                                                                                                                            >  2:1198233/1‑54 (MQ=255)

                                                                                aTGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGc                                                                                                                            <  1:1198233/54‑1 (MQ=255)

                                                                                 tGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                      <  2:97158/123‑1 (MQ=255)

                                                                                 tGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                      >  1:97158/1‑123 (MQ=255)

                                                                                 tGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAgg                                      >  1:32362/1‑139 (MQ=255)

                                                                                      ttATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgc                                 <  2:32362/139‑1 (MQ=255)

                                                                                         taACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               >  2:325817/1‑42 (MQ=255)

                                                                                         taACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                               <  1:325817/42‑1 (MQ=255)

                                                                                           aCTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                            <  1:1205941/139‑1 (MQ=255)

                                                                                           aCTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                            <  1:597569/139‑1 (MQ=255)

                                                                                           aCTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                            <  1:343620/139‑1 (MQ=255)

                                                                                             ttCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGc                                                                                                <  2:624130/69‑1 (MQ=255)

                                                                                             ttCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGc                                                                                                >  1:624130/1‑69 (MQ=255)

                                                                                             ttCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgct                          >  2:1491841/1‑139 (MQ=255)

                                                                                                cGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGa                                                                      <  1:784316/92‑1 (MQ=255)

                                                                                                cGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGa                                                                      >  2:784316/1‑92 (MQ=255)

                                                                                                cGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCggg                                               >  2:1497990/1‑115 (MQ=255)

                                                                                                cGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCggg                                               <  1:1497990/115‑1 (MQ=255)

                                                                                                cGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggc                       >  1:544367/1‑139 (MQ=255)

                                                                                                cGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggc                       >  2:1256575/1‑139 (MQ=255)

                                                                                                         tAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGt              <  1:1491841/139‑1 (MQ=255)

                                                                                                          aaGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTg             >  2:511265/1‑139 (MQ=255)

                                                                                                             cTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                 >  2:965198/1‑52 (MQ=255)

                                                                                                             cTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                 >  1:301487/1‑52 (MQ=255)

                                                                                                             cTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                 <  2:301487/52‑1 (MQ=255)

                                                                                                             cTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                 <  1:965198/52‑1 (MQ=255)

                                                                                                             cTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                      <  2:761052/95‑1 (MQ=255)

                                                                                                             cTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                      >  1:761052/1‑95 (MQ=255)

                                                                                                             cTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg          <  1:1256575/139‑1 (MQ=255)

                                                                                                               gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                 >  2:398661/1‑82 (MQ=255)

                                                                                                               gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                 <  1:398661/82‑1 (MQ=255)

                                                                                                               gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                >  2:755903/1‑99 (MQ=255)

                                                                                                               gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                <  1:755903/99‑1 (MQ=255)

                                                                                                                aaGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                  >  1:1567874/1‑80 (MQ=255)

                                                                                                                aaGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                  <  2:1567874/80‑1 (MQ=255)

                                                                                                                aaGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt       <  1:1456964/139‑1 (MQ=255)

                                                                                                                  gCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               >  2:622196/1‑49 (MQ=255)

                                                                                                                  gCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                               <  1:622196/49‑1 (MQ=255)

                                                                                                                     ttCATAACGTGAGTTGCCTATGCACAGTGTGGGATTCCCGCCGGCGCGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCg  >  2:1000402/1‑139 (MQ=255)

                                                                                                                     ttCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCg  >  2:90888/1‑139 (MQ=255)

TATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3767056‑3767311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A