Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,015	T→C	W329R (TGG→CGG)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,015	0	T	C	98.5%	231.4 / NA	68	W329R (TGG→CGG)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (34/33); total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACT  >  NC_000913/3969883‑3970147

aaTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCa                                                                                                                                   <  2:1456579/139‑1 (MQ=255)

aaTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCa                                                                                                                                   <  1:492822/139‑1 (MQ=255)

 aTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCAt                                                                                                                                  >  2:1519250/1‑139 (MQ=255)

 aTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCAt                                                                                                                                  <  1:160174/139‑1 (MQ=255)

     ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                     <  2:1770786/132‑1 (MQ=255)

     ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                     >  1:1770786/1‑132 (MQ=255)

     ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑c                                                                                                                             >  2:1325380/1‑139 (MQ=255)

                 cacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGAt                                                                                                                <  2:17398/139‑1 (MQ=255)

                    ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgg                                                                                                             >  2:510772/1‑139 (MQ=255)

                    ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgg                                                                                                             >  2:1773938/1‑139 (MQ=255)

                      tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCAtt                                                                                                                                 <  2:163689/119‑1 (MQ=255)

                      tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCAtt                                                                                                                                 >  1:163689/1‑119 (MQ=255)

                      tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgggg                                                                                                           >  2:512937/1‑139 (MQ=255)

                      tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgggg                                                                                                           >  2:29255/1‑139 (MQ=255)

                        aaTGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCt                                                                                                         >  1:338912/1‑139 (MQ=255)

                              ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTc                                                                                                   >  2:466753/1‑139 (MQ=255)

                                 ccAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCa                                                                                                <  1:29255/139‑1 (MQ=255)

                                    aCCCTGGATCCGCGTTTTCAGACCTATCACTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTa                                                                                             >  1:1166713/1‑139 (MQ=255)

                                               gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCa                                                                                                                                   >  1:1557019/1‑92 (MQ=255)

                                               gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCa                                                                                                                                   <  2:1557019/92‑1 (MQ=255)

                                                cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                 >  2:239018/1‑139 (MQ=255)

                                                cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                 >  1:227001/1‑139 (MQ=255)

                                                 gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                <  2:227001/139‑1 (MQ=255)

                                                  ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                               <  1:1519250/139‑1 (MQ=255)

                                                  ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                               <  2:338912/139‑1 (MQ=255)

                                                  ataagggacagaTCGTTATTGGCGAACGCCGGAAGACCCGGTAAAACGCGATAGCCCACTTCGGGCCTTCCTGATGATTATGCGGGGCATTTT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                               <  1:98791/128‑1 (MQ=255)

                                                       aGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt                                                                          <  1:1325380/139‑1 (MQ=255)

                                                             aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                    >  2:907514/1‑139 (MQ=255)

                                                             aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                    >  1:1355507/1‑139 (MQ=255)

                                                             aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑C‑gggg‑ggg                                                                                                                    <  1:600255/90‑2 (MQ=255)

                                                             aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑C‑gggg‑ggg                                                                                                                    >  2:600255/1‑89 (MQ=255)

                                                                   aTTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActgct                                                              >  1:989281/1‑139 (MQ=255)

                                                                     ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtgctgc                                                            >  2:1037030/1‑139 (MQ=255)

                                                                        cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAc                                                                                           <  1:644376/105‑1 (MQ=255)

                                                                        cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAc                                                                                           >  2:644376/1‑105 (MQ=255)

                                                                        cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaacctgtctcttat                                                         >  2:98791/1‑128 (MQ=255)

                                                                                       ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                          <  2:989281/139‑1 (MQ=255)

                                                                                       ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                          <  1:512937/139‑1 (MQ=255)

                                                                                          gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                       <  2:1166713/139‑1 (MQ=255)

                                                                                                cgcgATAGCCCACGTCATGCCTTCCTGATGATTATGTGGGGCATTGTGG‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGCTGCTCGAAACAACCACACTGCTGCGGTGAGCGTAAACGCGCTCGACGCttc                                 >  1:439635/1‑138 (MQ=255)

                                                                                                    aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGAt                                                                                                                <  1:848991/56‑1 (MQ=255)

                                                                                                    aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGAt                                                                                                                >  2:848991/1‑56 (MQ=255)

                                                                                                    aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                             <  1:1037030/139‑1 (MQ=255)

                                                                                                    aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                             >  1:372481/1‑139 (MQ=255)

                                                                                                     tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                   <  2:687386/100‑1 (MQ=255)

                                                                                                     tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                   >  1:687386/1‑100 (MQ=255)

                                                                                                          cACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAtt                               >  1:1504591/1‑131 (MQ=255)

                                                                                                          cACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAtt                               <  2:1504591/131‑1 (MQ=255)

                                                                                                           aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                     <  1:848195/92‑1 (MQ=255)

                                                                                                           aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                     >  2:848195/1‑92 (MQ=255)

                                                                                                               cgtGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                  >  2:1181279/1‑139 (MQ=255)

                                                                                                               cgtGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                  <  1:1773938/139‑1 (MQ=255)

                                                                                                                 tGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                >  2:1224611/1‑123 (MQ=255)

                                                                                                                 tGCCTTCCTGATGATTATGCGGGGCATTGT‑C‑GGGGTGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                <  1:1224611/123‑1 (MQ=255)

                                                                                                                  gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                <  2:1080808/74‑1 (MQ=255)

                                                                                                                  gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                >  2:309330/1‑74 (MQ=255)

                                                                                                                  gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                <  1:309330/74‑1 (MQ=255)

                                                                                                                  gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                >  1:1080808/1‑74 (MQ=255)

                                                                                                                    cTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa             <  1:1181279/139‑1 (MQ=255)

                                                                                                                       ccTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                   >  1:94096/1‑114 (MQ=255)

                                                                                                                       ccTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                   <  2:94096/114‑1 (MQ=255)

                                                                                                                           atgatTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact      >  2:413183/1‑139 (MQ=255)

                                                                                                                            tgatTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg     <  2:372481/139‑1 (MQ=255)

                                                                                                                             gatTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga    <  1:907514/139‑1 (MQ=255)

                                                                                                                              atTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGTAAAGGAGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac   <  1:239018/139‑1 (MQ=255)

                                                                                                                              atTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac   >  2:1462504/1‑139 (MQ=255)

                                                                                                                              atTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac   <  2:1355507/139‑1 (MQ=255)

                                                                                                                                tATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact  >  2:414364/1‑138 (MQ=255)

                                                                                                                                tATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact  <  1:414364/138‑1 (MQ=255)

AATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACT  >  NC_000913/3969883‑3970147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A