Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,970,015 T→C W329R (TGG→CGG)  wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,970,0150TC100.0% 214.2 / NA 61W329R (TGG→CGG) wzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/30);  total (31/30)

CGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG  >  NC_000913/3969886‑3970148
                                                                                                                                 |                                                                                                                                       
cGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTg                                                                                                                                >  1:400472/1‑139 (MQ=255)
             acacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑gggggggctga                                                                                                                 <  1:1406126/139‑6 (MQ=255)
                 ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGt                                                                                                                               <  2:222259/123‑1 (MQ=255)
                 ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGt                                                                                                                               >  1:222259/1‑123 (MQ=255)
                 ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgg                                                                                                              >  2:463068/1‑139 (MQ=255)
                 ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgg                                                                                                              >  2:1316447/1‑139 (MQ=255)
                 ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgg                                                                                                              >  1:132133/1‑139 (MQ=255)
                           ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGgtgt                                                                                                     >  1:1100051/1‑139 (MQ=255)
                           ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTc                                                                                                    >  2:647281/1‑139 (MQ=255)
                           ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTc                                                                                                    >  2:1119748/1‑139 (MQ=255)
                           ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCAAGTCGTGCCTTCCTGATGATTATCCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTc                                                                                                    >  2:962471/1‑139 (MQ=255)
                             tCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGc                                                                                                  <  2:75713/139‑1 (MQ=255)
                                         tCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgccg                                                                                      <  1:142789/139‑1 (MQ=255)
                                            gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGc                                                                                                           <  2:1209518/115‑1 (MQ=255)
                                            gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGc                                                                                                           >  1:1209518/1‑115 (MQ=255)
                                            gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg                                                                                   >  1:280076/1‑139 (MQ=255)
                                            gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg                                                                                   >  1:721646/1‑139 (MQ=255)
                                              gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                 <  1:962471/139‑1 (MQ=255)
                                               ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                <  1:1316447/139‑1 (MQ=255)
                                                 ttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                              <  2:721646/139‑1 (MQ=255)
                                                 ttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                              <  2:280076/139‑1 (MQ=255)
                                                  tCAGACCTATTGCTATTTGCGTCCGCAGGAAGAACCGGTAAACCGCGATAGCCCACGTGGTGTCTTCCAAATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                              <  2:1100051/139‑1 (MQ=255)
                                                          aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑GGGGGGGCTATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                  <  1:1265094/125‑1 (MQ=255)
                                                          aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑GGGGGGGCTATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                  >  2:1265094/1‑125 (MQ=255)
                                                                   tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                            >  2:468102/1‑139 (MQ=255)
                                                                   tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                            >  2:721841/1‑139 (MQ=255)
                                                                     cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt                                                          <  2:400472/139‑1 (MQ=255)
                                                                         cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtg                                                                                                      <  1:1143630/91‑1 (MQ=255)
                                                                         cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtg                                                                                                      >  2:1143630/1‑91 (MQ=255)
                                                                            cGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCa                                                   <  1:721841/139‑1 (MQ=255)
                                                                               aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgggg                                                                                                            <  1:156574/79‑1 (MQ=255)
                                                                               aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgggg                                                                                                            <  1:852362/79‑1 (MQ=255)
                                                                               aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgggg                                                                                                            >  2:852362/1‑79 (MQ=255)
                                                                               aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgggg                                                                                                            >  2:156574/1‑79 (MQ=255)
                                                                                    ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTg                                                         >  1:850951/1‑125 (MQ=255)
                                                                                    ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTg                                                         <  2:850951/125‑1 (MQ=255)
                                                                                       gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                        >  1:986664/1‑139 (MQ=255)
                                                                                       gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                        >  2:237959/1‑139 (MQ=255)
                                                                                       gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                        <  1:647281/139‑1 (MQ=255)
                                                                                        tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                              <  1:1248936/100‑1 (MQ=255)
                                                                                        tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                              >  2:1248936/1‑100 (MQ=255)
                                                                                         aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                      <  1:237959/139‑1 (MQ=255)
                                                                                           aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGTTGTCGTATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                    <  2:132133/139‑1 (MQ=255)
                                                                                           aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                    <  1:468102/139‑1 (MQ=255)
                                                                                             cgcgATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                    <  1:1068618/137‑1 (MQ=255)
                                                                                             cgcgATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                    >  2:1068618/1‑137 (MQ=255)
                                                                                                 aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtg                                                                                                      >  2:1183323/1‑67 (MQ=255)
                                                                                                 aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtg                                                                                                      <  1:1183323/67‑1 (MQ=255)
                                                                                                 aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                              <  1:1119748/139‑1 (MQ=255)
                                                                                                  tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGa                             >  1:214067/1‑139 (MQ=255)
                                                                                                          gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                     >  1:979576/1‑139 (MQ=255)
                                                                                                              tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGAt                                                                                                                 <  2:1353360/43‑1 (MQ=255)
                                                                                                              tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGAt                                                                                                                 >  1:1353360/1‑43 (MQ=255)
                                                                                                              tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                           <  2:1246405/113‑1 (MQ=255)
                                                                                                              tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                           >  1:1246405/1‑113 (MQ=255)
                                                                                                              tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgc                                     <  2:283255/119‑1 (MQ=255)
                                                                                                              tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgc                                     >  1:283255/1‑119 (MQ=255)
                                                                                                              tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                 <  1:463068/139‑1 (MQ=255)
                                                                                                                        atgatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                >  2:1016494/1‑66 (MQ=255)
                                                                                                                        atgatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                <  1:1016494/66‑1 (MQ=255)
                                                                                                                             tATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg  >  2:728496/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                       
CGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG  >  NC_000913/3969886‑3970148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A