Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,414	G→A	475 (TAG→TAA)	ascB →	cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,414	0	G	A	100.0%	183.9 / NA	57	475 (TAG→TAA)	ascB	cryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/29); total (28/29)

AGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAA  >  NC_000913/2842286‑2842543

aGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGc                                                                                                                         >  2:481515/1‑139 (MQ=255)

  cAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCg                                                                                                                       >  1:180970/1‑139 (MQ=255)

                 tgtctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACg                                                                                                        >  1:747676/1‑139 (MQ=255)

                  gtctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGc                                                                                                       <  2:383390/139‑1 (MQ=255)

                        gttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAgtaagtaa                                                                                                                               >  1:1014411/1‑109 (MQ=255)

                        gttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAgtaagtaa                                                                                                                               >  1:1368268/1‑109 (MQ=255)

                        gttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAgtaagtaa                                                                                                                               <  2:1368268/109‑1 (MQ=255)

                        gttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAgtaagtaa                                                                                                                               <  2:1014411/109‑1 (MQ=255)

                           gACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                            <  2:144484/125‑1 (MQ=255)

                           gACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                            >  1:144484/1‑125 (MQ=255)

                               gTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACg                                                                                                        >  2:1015684/1‑125 (MQ=255)

                               gTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACg                                                                                                        <  1:1015684/125‑1 (MQ=255)

                                   cgacgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGcc                                                                                                      >  2:1406083/1‑123 (MQ=255)

                                   cgacgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGcc                                                                                                      <  1:1406083/123‑1 (MQ=255)

                                        cAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                 <  2:180970/139‑1 (MQ=255)

                                        cAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                 <  1:26189/139‑1 (MQ=255)

                                                     gCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                 <  1:261244/110‑1 (MQ=255)

                                                     gCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                 >  2:261244/1‑110 (MQ=255)

                                                      cTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGc                                                                   <  1:481515/139‑1 (MQ=255)

                                                          cgcgCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGa                                                               >  1:1148144/1‑139 (MQ=255)

                                                            cgcACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATa                                                             >  2:1029995/1‑139 (MQ=255)

                                                            cgcACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATa                                                             >  2:626771/1‑139 (MQ=255)

                                                                cgcgTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                 <  2:820086/115‑1 (MQ=255)

                                                                cgcgTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                 >  1:820086/1‑115 (MQ=255)

                                                                cgcgTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAagcg                                                         >  2:1071979/1‑139 (MQ=255)

                                                                   gTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                            <  2:1291689/85‑1 (MQ=255)

                                                                   gTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                            >  1:1291689/1‑85 (MQ=255)

                                                                   gTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGc                                                                   <  1:586561/126‑1 (MQ=255)

                                                                   gTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGc                                                                   >  2:586561/1‑126 (MQ=255)

                                                                           tCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                              >  1:477606/1‑139 (MQ=255)

                                                                            cATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                             <  1:1029995/139‑1 (MQ=255)

                                                                             aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  <  2:1301624/85‑1 (MQ=255)

                                                                             aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  <  1:119265/85‑1 (MQ=255)

                                                                             aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  >  2:119265/1‑85 (MQ=255)

                                                                             aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  >  1:1301624/1‑85 (MQ=255)

                                                                                       tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                             <  1:854226/128‑1 (MQ=255)

                                                                                       tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                             >  2:854226/1‑128 (MQ=255)

                                                                                        ataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGa                                                                                                           <  2:1005297/65‑1 (MQ=255)

                                                                                        ataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGa                                                                                                           >  1:1005297/1‑65 (MQ=255)

                                                                                        ataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTa                                      <  1:435364/134‑1 (MQ=255)

                                                                                        ataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTa                                      >  2:435364/1‑134 (MQ=255)

                                                                                         taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAAc                                                                                                                              <  1:290376/45‑1 (MQ=255)

                                                                                         taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAAc                                                                                                                              >  2:290376/1‑45 (MQ=255)

                                                                                               aGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATc                          <  2:747676/139‑1 (MQ=255)

                                                                                                gTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                 >  1:687576/1‑83 (MQ=255)

                                                                                                gTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                 <  2:687576/83‑1 (MQ=255)

                                                                                                 tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTa                                                                                                   <  1:1253764/64‑1 (MQ=255)

                                                                                                 tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTa                                                                                                   >  2:1253764/1‑64 (MQ=255)

                                                                                                 tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  >  1:106644/1‑65 (MQ=255)

                                                                                                 tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  <  2:106644/65‑1 (MQ=255)

                                                                                                             aTGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCAcc            <  2:477606/139‑1 (MQ=255)

                                                                                                                gggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCa         <  1:626771/139‑1 (MQ=255)

                                                                                                                 ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                <  2:1466705/51‑1 (MQ=255)

                                                                                                                 ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                >  1:1466705/1‑51 (MQ=255)

                                                                                                                 ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGc                     <  2:283665/126‑1 (MQ=255)

                                                                                                                 ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGc                     >  1:283665/1‑126 (MQ=255)

                                                                                                                 ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCAt        <  2:233290/139‑1 (MQ=255)

                                                                                                                 ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCAt        >  1:233290/1‑139 (MQ=255)

                                                                                                                     gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                              >  2:236974/1‑97 (MQ=255)

                                                                                                                     gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                              <  1:236974/97‑1 (MQ=255)

                                                                                                                       tttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGaa  <  1:1071979/139‑1 (MQ=255)

AGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAA  >  NC_000913/2842286‑2842543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A