Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,414	G→A	475 (TAG→TAA)	ascB →	cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,414	0	G	A	100.0%	164.8 / NA	52	475 (TAG→TAA)	ascB	cryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/24); total (28/24)

CAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCC  >  NC_000913/2842288‑2842549

cAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCg                                                                                                                             >  1:792243/1‑139 (MQ=255)

  aaCGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGa                                                                                                                           >  1:848153/1‑139 (MQ=255)

      cTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATgcg                                                                                                                       <  2:848153/139‑1 (MQ=255)

      cTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAAAGGAAGTGACAGTGCCGGATgcg                                                                                                                       <  2:1015706/139‑1 (MQ=255)

           gCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                                  <  2:449688/139‑1 (MQ=255)

              ttgtctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAAc                                                                                                               >  2:197880/1‑139 (MQ=255)

               tgtctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACg                                                                                                              >  2:1536497/1‑139 (MQ=255)

                           ccGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                                  >  1:139535/1‑123 (MQ=255)

                           ccGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                                  <  2:139535/123‑1 (MQ=255)

                                acgacgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAt                                                                                             >  2:637228/1‑139 (MQ=255)

                                      cAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                       <  1:1495166/139‑1 (MQ=255)

                                         gCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGa                                                                                    >  1:141742/1‑139 (MQ=255)

                                          cAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGaa                                                                                   <  2:998542/139‑1 (MQ=255)

                                               gTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATg                                                                              <  2:1554830/139‑1 (MQ=255)

                                                 aCGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGaa                                                                            >  1:160425/1‑139 (MQ=255)

                                                       aCGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCg                                                                      >  2:1228926/1‑139 (MQ=255)

                                                                          cATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                   <  2:160425/139‑1 (MQ=255)

                                                                                ggtggtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAAttt                                             >  2:612927/1‑139 (MQ=255)

                                                                                     tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGAt                                                                                                                          >  1:555566/1‑57 (MQ=255)

                                                                                     tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGAt                                                                                                                          <  2:555566/57‑1 (MQ=255)

                                                                                     tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGTGGCGTGAACGCCTTATcc                                                                                                      <  1:815730/77‑1 (MQ=255)

                                                                                     tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGTGGCGTGAACGCCTTATcc                                                                                                      >  2:815730/1‑77 (MQ=255)

                                                                                       taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                   <  1:1491346/126‑1 (MQ=255)

                                                                                       taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                   >  2:1491346/1‑126 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAATTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                   <  2:1494470/118‑1 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                       >  2:676547/1‑82 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                       <  1:676547/82‑1 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGaa                                                                                   >  2:1578753/1‑86 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGaa                                                                                   <  1:1578753/86‑1 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                   >  1:186755/1‑118 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                   <  2:186755/118‑1 (MQ=255)

                                                                                               tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                   >  1:1494470/1‑118 (MQ=255)

                                                                                                 aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                                  <  2:746080/53‑1 (MQ=255)

                                                                                                 aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                                  >  1:746080/1‑53 (MQ=255)

                                                                                                 aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAag                                                                 <  1:1224885/102‑1 (MQ=255)

                                                                                                 aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAag                                                                 >  2:1224885/1‑102 (MQ=255)

                                                                                                 aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCagag                            <  2:685090/139‑1 (MQ=255)

                                                                                                    gCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTg                         >  1:290031/1‑139 (MQ=255)

                                                                                                         tAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCa                    >  2:1224991/1‑139 (MQ=255)

                                                                                                         tAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGACTACATGGCGCACGAACCATGAACGCCCGATAAGCGCAGCGCATCCGGCAATTTAGCATTTGGCATCAGAGCTGGTTCa                    >  2:154487/1‑139 (MQ=38)

                                                                                                           aTGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCAcc                  <  1:1228926/139‑1 (MQ=255)

                                                                                                              gggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGa                                                                                                                 >  2:1578065/1‑41 (MQ=255)

                                                                                                              gggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGa                                                                                                                 <  1:1578065/41‑1 (MQ=255)

                                                                                                              gggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCa               >  1:579152/1‑139 (MQ=255)

                                                                                                                gAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                       >  1:704482/1‑65 (MQ=255)

                                                                                                                gAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                       <  2:704482/65‑1 (MQ=255)

                                                                                                                  aGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCaa           >  1:1430969/1‑139 (MQ=255)

                                                                                                                   gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAg          >  1:835509/1‑139 (MQ=255)

                                                                                                                   gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAg          >  1:1260318/1‑139 (MQ=255)

                                                                                                                     tttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGaa        <  2:1607254/139‑1 (MQ=255)

                                                                                                                     tttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGaa        <  2:141742/139‑1 (MQ=255)

                                                                                                                     tttAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGaa        <  2:835509/139‑1 (MQ=255)

                                                                                                                          agtaagtaACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATcc   <  1:931082/139‑1 (MQ=255)

                                                                                                                           gtaagtaaCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATccc  <  1:612927/139‑1 (MQ=255)

CAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCC  >  NC_000913/2842288‑2842549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A