Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,414	G→A	475 (TAG→TAA)	ascB →	cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,414	0	G	A	100.0%	65.2 / NA	21	475 (TAG→TAA)	ascB	cryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/8); total (13/8)

AAATGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAG  >  NC_000913/2842281‑2842521

aaaTGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGAGGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAAc                                                                                                        >  2:739246/1‑139 (MQ=255)

  aTGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAg                                                                                                      >  1:599450/1‑139 (MQ=255)

             cTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATgcg                                                                                           <  1:739246/139‑1 (MQ=255)

                             gttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                            >  1:1035936/1‑138 (MQ=255)

                             gttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                            <  2:1035936/138‑1 (MQ=255)

                                                       tACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGagtaagta                                                                                                          <  1:254950/82‑1 (MQ=255)

                                                       tACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGagtaagta                                                                                                          >  2:254950/1‑82 (MQ=255)

                                                                   cACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAag                                     >  1:854748/1‑139 (MQ=255)

                                                                                  aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                       <  1:206847/138‑1 (MQ=255)

                                                                                  aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                       >  2:206847/1‑138 (MQ=255)

                                                                                        gtggtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTa                >  2:245464/1‑139 (MQ=255)

                                                                                         tggtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAg               <  1:245464/139‑1 (MQ=255)

                                                                                           gtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGaa                                                <  2:881251/104‑1 (MQ=255)

                                                                                           gtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGaa                                                >  1:881251/1‑104 (MQ=255)

                                                                                            aataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAt            >  2:708645/2‑139 (MQ=255)

                                                                                              taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAttt          >  2:413587/1‑139 (MQ=255)

                                                                                              taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAttt          >  2:630627/1‑139 (MQ=255)

                                                                                                    aGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATc    <  1:413587/139‑1 (MQ=255)

                                                                                                     gTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                           >  1:445706/1‑83 (MQ=255)

                                                                                                     gTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                           <  2:445706/83‑1 (MQ=255)

                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATgcg                                                                                           >  2:251676/1‑50 (MQ=255)

                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATgcg                                                                                           <  1:251676/50‑1 (MQ=255)

                                                                                                      tGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCag  >  1:856325/1‑139 (MQ=255)

AAATGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAG  >  NC_000913/2842281‑2842521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A