Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I2 R1 758 59.3 2864096 93.9% 2689386 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,205,324 C→T *311* (TAG→TAA)  ttdR ← transcriptional activator of ttdABT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,205,3240CT100.0% 186.6 / NA 58*311* (TAG→TAA) ttdRtranscriptional activator of ttdABT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/28);  total (30/28)

GTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCTT  >  NC_000913/3205189‑3205456
                                                                                                                                       |                                                                                                                                    
gTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTAc                                                                                                                                   <  2:821874/139‑1 (MQ=255)
   tCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATg                                                                                                                                <  2:495702/139‑1 (MQ=255)
                   tGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                <  2:550102/139‑1 (MQ=255)
                          gctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCtt                                                                                                         >  2:108450/1‑139 (MQ=255)
                           ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTg                                                                                                        >  2:978703/1‑139 (MQ=255)
                           ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTg                                                                                                        >  2:131105/1‑139 (MQ=255)
                                tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  >  2:136399/1‑108 (MQ=255)
                                tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  <  1:136399/108‑1 (MQ=255)
                                tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                   >  2:1388915/1‑139 (MQ=255)
                                tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                   >  1:722329/1‑139 (MQ=255)
                                tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATGCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                   >  1:45296/1‑139 (MQ=255)
                                  atcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc                                                                                                 <  1:108450/139‑1 (MQ=255)
                                   tcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCg                                                                                                <  2:45296/139‑1 (MQ=255)
                                          aacaTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTgg                                                                                         >  1:148821/1‑139 (MQ=255)
                                            caTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCa                                                                                       >  1:235497/1‑139 (MQ=255)
                                              tCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCAcc                                                                                     >  1:1091628/1‑139 (MQ=255)
                                              tCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCAcc                                                                                     >  2:746734/1‑139 (MQ=255)
                                                cAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCAt                                                                                   >  2:844198/1‑139 (MQ=255)
                                                       tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCa                                                                            >  1:222723/1‑139 (MQ=255)
                                                       tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCa                                                                            >  2:1158994/1‑139 (MQ=255)
                                                        gtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAg                                                                           <  2:235497/139‑1 (MQ=255)
                                                            cgtcgtCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCCGCCGTTGCTGGCACCATGCCGCCAGAAAt                                                                       >  1:1387214/1‑139 (MQ=255)
                                                                gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  >  1:1202626/1‑76 (MQ=255)
                                                                gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  <  2:1202626/76‑1 (MQ=255)
                                                                gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa                                                                   <  1:709584/139‑1 (MQ=255)
                                                                gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa                                                                   >  2:606763/1‑139 (MQ=255)
                                                                gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa                                                                   >  2:1223526/1‑139 (MQ=255)
                                                                 tcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTTTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                >  2:293146/1‑93 (MQ=255)
                                                                 tcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTTTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                <  1:293146/93‑1 (MQ=255)
                                                                  cAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACg                                                                 <  1:1223526/139‑1 (MQ=255)
                                                                      agaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                             <  2:1091628/139‑1 (MQ=255)
                                                                      agaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                             <  2:722329/139‑1 (MQ=255)
                                                                       gaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGac                                                            <  2:222723/139‑1 (MQ=255)
                                                                        aCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGaca                                                           >  1:405501/1‑139 (MQ=255)
                                                                        aCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGaca                                                           >  1:277158/1‑139 (MQ=255)
                                                                          aaaaaTCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACg                                                         <  1:1388915/139‑1 (MQ=255)
                                                                          aaaaaTCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACg                                                         <  1:746734/139‑1 (MQ=255)
                                                                                cTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAAttt                                                   <  2:148821/139‑1 (MQ=255)
                                                                                    aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               <  1:606763/139‑1 (MQ=255)
                                                                                    aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               <  1:844198/139‑1 (MQ=255)
                                                                                    aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               >  1:657613/1‑139 (MQ=255)
                                                                                     cGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtgc                                              <  2:177424/139‑1 (MQ=255)
                                                                                               aagaaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATagag                                    <  1:1158994/139‑1 (MQ=255)
                                                                                                agaaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTG‑CCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCg                                  <  1:131105/139‑1 (MQ=255)
                                                                                                    agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCt                               >  2:201439/1‑139 (MQ=255)
                                                                                                      cgcgAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTcc                             >  2:549227/1‑139 (MQ=255)
                                                                                                       gcgAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTccc                            >  2:115814/1‑139 (MQ=255)
                                                                                                             aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                      <  1:841485/139‑1 (MQ=255)
                                                                                                              ggATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAAc                     <  2:1292470/139‑1 (MQ=255)
                                                                                                                aTCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtgc                                              <  2:34558/112‑1 (MQ=255)
                                                                                                                aTCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtgc                                              >  1:34558/1‑112 (MQ=255)
                                                                                                                       gTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                <  1:15881/39‑1 (MQ=255)
                                                                                                                       gTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                >  2:15881/1‑39 (MQ=255)
                                                                                                                          aTTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               >  1:507674/1‑101 (MQ=255)
                                                                                                                          aTTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               <  2:507674/101‑1 (MQ=255)
                                                                                                                                 gtggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCtt  >  2:567109/1‑139 (MQ=255)
                                                                                                                                  tggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCa                                                               <  2:1036053/77‑1 (MQ=255)
                                                                                                                                  tggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCa                                                               >  1:1036053/1‑77 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCTT  >  NC_000913/3205189‑3205456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A