Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,205,324 |
C→T |
*311* (TAG→TAA) |
ttdR ← |
transcriptional activator of ttdABT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,205,324 | 0 | C | T | 100.0%
| 84.6
/ NA
| 27 | *311* (TAG→TAA) | ttdR | transcriptional activator of ttdABT |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/11); total (16/11) |
ATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCTT > NC_000913/3205195‑3205456
|
aTGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGAcc > 1:526015/1‑139 (MQ=255)
ctctTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATa > 2:521394/1‑139 (MQ=255)
gcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGcc > 2:944994/1‑139 (MQ=255)
atcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc < 1:30073/139‑1 (MQ=255)
atGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGtt > 1:316234/1‑139 (MQ=255)
acaacaTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCt > 1:879136/1‑139 (MQ=255)
cGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATgcc > 1:1001311/1‑139 (MQ=255)
gtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc > 1:539834/1‑117 (MQ=255)
gtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc < 2:539834/117‑1 (MQ=255)
tgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGa > 2:831997/1‑139 (MQ=255)
tcgtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTc > 1:232521/1‑139 (MQ=255)
gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa > 2:191227/1‑139 (MQ=255)
gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa > 2:420748/1‑139 (MQ=255)
cGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtgc < 1:944994/139‑1 (MQ=255)
tCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGcc < 1:323298/77‑1 (MQ=255)
tCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGcc > 2:323298/1‑77 (MQ=255)
aaagCGCGAAAAGGATCCGGAGGGATTTCTGGTGGATTTACTTGACCTAATGGCCTTAATCGGGTTGGCCCAGCCGTTGCTGGCCCCATGCCGCCAGAAATTCCACGCAAACACTAAATTTCATGCTGCGATAGGGCgc > 1:746926/1‑138 (MQ=255)
aaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACAt < 2:200477/43‑1 (MQ=255)
aaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACAt > 1:200477/1‑43 (MQ=255)
aagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGc < 1:831997/139‑1 (MQ=255)
agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCt > 1:446101/1‑139 (MQ=255)
cgAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCg < 2:526015/139‑1 (MQ=255)
gATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACa < 2:879136/139‑1 (MQ=255)
tggtggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGgcgc < 2:232521/139‑1 (MQ=255)
gtggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCtt < 1:115774/139‑1 (MQ=255)
tggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGc < 2:820881/108‑1 (MQ=255)
tggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGc > 1:820881/1‑108 (MQ=255)
|
ATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCTT > NC_000913/3205195‑3205456
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
