Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,902,543 |
G→A |
G216G (GGG→GGA) |
ygcE → |
putative kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,902,543 | 0 | G | A | 100.0%
| 184.4
/ NA
| 57 | G216G (GGG→GGA) | ygcE | putative kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/25); total (32/25) |
TTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCT > NC_000913/2902418‑2902677
|
tttGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTgg > 2:909593/1‑139 (MQ=255)
aTGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGgatga > 2:437305/1‑139 (MQ=255)
gCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaa > 2:656225/1‑139 (MQ=255)
aaaTGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAc > 1:463356/1‑139 (MQ=255)
gCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAAt > 2:185847/1‑139 (MQ=255)
tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg < 2:182077/105‑1 (MQ=255)
tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg > 1:182077/1‑105 (MQ=255)
tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcgg > 2:443495/1‑126 (MQ=255)
tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcgg < 1:443495/126‑1 (MQ=255)
tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTctct > 1:519539/1‑139 (MQ=255)
ccTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCtt < 1:656225/139‑1 (MQ=255)
cTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTg < 2:463356/139‑1 (MQ=255)
tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTAc > 1:859167/1‑139 (MQ=255)
atCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACa > 1:180120/1‑139 (MQ=255)
acacCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCg < 1:185847/139‑1 (MQ=255)
accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaa > 2:737808/1‑106 (MQ=255)
accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaa < 1:737808/106‑1 (MQ=255)
ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc < 2:1105255/86‑1 (MQ=255)
ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc > 1:1105255/1‑86 (MQ=255)
ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCAt < 1:904368/139‑1 (MQ=255)
acaAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATtg > 2:158310/1‑139 (MQ=255)
caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGcc < 2:505035/84‑1 (MQ=255)
caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGcc > 1:505035/1‑84 (MQ=255)
caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATtga < 2:180120/139‑1 (MQ=255)
gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc > 1:1089857/1‑80 (MQ=255)
gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc < 2:1089857/80‑1 (MQ=255)
gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGAt < 2:715563/85‑1 (MQ=255)
gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGAt > 1:715563/1‑85 (MQ=255)
aCTGGCGACACATTTCCCGGCAGGCCTGCCGGTTGTTTGTACCACCAGTGCTAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTCATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAAc > 2:784971/1‑139 (MQ=255)
tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAc > 1:800972/1‑82 (MQ=255)
tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAc < 2:800972/82‑1 (MQ=255)
tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTg < 1:1005542/137‑1 (MQ=255)
tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTg > 2:1005542/1‑137 (MQ=255)
cGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa < 1:158310/139‑1 (MQ=255)
ggACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATc < 2:519539/139‑1 (MQ=255)
gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa > 1:521173/1‑139 (MQ=255)
gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa > 2:1129032/1‑125 (MQ=255)
gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa < 1:1129032/125‑1 (MQ=255)
gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATcc > 1:592700/1‑139 (MQ=255)
gCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTa < 1:909593/139‑1 (MQ=255)
accaccAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa > 2:377577/1‑108 (MQ=255)
accaccAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa < 1:377577/108‑1 (MQ=255)
ccaccaGTGATAAACAGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTg < 2:521173/139‑1 (MQ=255)
accaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa < 2:183145/105‑1 (MQ=255)
accaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCa > 1:183145/1‑105 (MQ=255)
caGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGc > 1:1033132/1‑131 (MQ=255)
caGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGc < 2:1033132/131‑1 (MQ=255)
gATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt > 1:558866/1‑139 (MQ=255)
gATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt > 1:1059703/1‑139 (MQ=255)
aTAAACCGGTAGAAGCTCTGGGAGCCGGTTTACTCGATGATGAAACGCCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTCTTGATTAACGGCAAAGCACTGCCTAAAGATCCAGTCGCGCACTGGCCGATTAGCtcttc > 2:565714/1‑139 (MQ=255)
aCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt > 1:396162/1‑134 (MQ=255)
aCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt < 2:396162/134‑1 (MQ=255)
gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTa > 1:136914/1‑62 (MQ=255)
gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTa < 2:136914/62‑1 (MQ=255)
gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCg > 2:129984/1‑96 (MQ=255)
gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCg < 1:129984/96‑1 (MQ=255)
tctGGGAGCCGGATTACTGGATGATGAAACGGCGCTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg < 1:565714/139‑1 (MQ=255)
tGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCt > 2:1041441/1‑139 (MQ=255)
|
TTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGG‑‑‑‑‑‑CACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCT > NC_000913/2902418‑2902677
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
