Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,902,543	G→A	G216G (GGG→GGA)	ygcE →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,902,543	0	G	A	100.0%	164.5 / NA	53	G216G (GGG→GGA)	ygcE	putative kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/26); total (27/26)

GTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCT  >  NC_000913/2902407‑2902677

gTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCTGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                      <  1:477047/139‑1 (MQ=255)

gTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                      <  1:381711/139‑1 (MQ=255)

 tCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGcc                                                                                                                                     <  2:739977/139‑1 (MQ=255)

       gCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTa                                                                                                                               <  2:383144/139‑1 (MQ=255)

            ttGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGga                                                                                                                          <  2:113119/139‑1 (MQ=255)

                            tGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTa                                                                                                          >  2:44354/1‑139 (MQ=255)

                                 ccGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtc                                                                                                     >  1:60123/1‑139 (MQ=255)

                                   gTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                   <  2:585638/139‑1 (MQ=255)

                                   gTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                   <  1:57407/139‑1 (MQ=255)

                                    tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTctct                                                                                                  >  1:67036/1‑139 (MQ=255)

                                    tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTctct                                                                                                  >  1:320538/1‑139 (MQ=255)

                                     ccTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCtt                                                                                                 <  2:457756/139‑1 (MQ=255)

                                            cATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATGTCTCTTGGCACtt                                                                                          <  2:582573/139‑1 (MQ=255)

                                                    accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACa                                                                                       >  2:578305/1‑134 (MQ=255)

                                                    accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACa                                                                                       <  1:578305/134‑1 (MQ=255)

                                                    accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCa                                                                                  >  1:93054/1‑139 (MQ=255)

                                                    accacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCa                                                                                  >  2:155328/1‑139 (MQ=255)

                                                     ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAt                                                                                 >  1:672647/1‑139 (MQ=255)

                                                      cacaAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAtt                                                                                >  2:591806/1‑139 (MQ=255)

                                                       acaAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtg                                                                               >  1:593036/1‑139 (MQ=255)

                                                        caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcggcg                                                                                                             >  1:374922/1‑108 (MQ=255)

                                                        caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcggcg                                                                                                             <  2:374922/108‑1 (MQ=255)

                                                         aaGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgat                                                                             >  1:256647/1‑139 (MQ=255)

                                                          aGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCtt                                                                                                 >  1:618876/1‑118 (MQ=255)

                                                          aGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCtt                                                                                                 <  2:618876/118‑1 (MQ=255)

                                                           gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGAt                                                                                                                                 >  2:665762/1‑85 (MQ=255)

                                                           gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGAt                                                                                                                                 <  1:665762/85‑1 (MQ=255)

                                                                    gCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCa                                                                  <  2:60123/139‑1 (MQ=255)

                                                                              tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc                                                        >  2:233111/1‑139 (MQ=255)

                                                                                 cGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa                                                     <  2:256647/139‑1 (MQ=255)

                                                                                 cGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa                                                     <  2:593036/139‑1 (MQ=255)

                                                                                 cGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa                                                     <  2:696317/139‑1 (MQ=255)

                                                                                   gCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACAt                                                                                      >  1:506737/1‑104 (MQ=255)

                                                                                   gCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACAt                                                                                      <  2:506737/104‑1 (MQ=255)

                                                                                                    ttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTgg                                  <  1:233111/139‑1 (MQ=255)

                                                                                                          caccaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGAtt                            <  1:44354/139‑1 (MQ=255)

                                                                                                             caGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACAt                                                                                      <  2:616518/78‑1 (MQ=255)

                                                                                                             caGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACAt                                                                                      >  1:616518/1‑78 (MQ=255)

                                                                                                               gTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtc                       >  1:701332/1‑139 (MQ=255)

                                                                                                                    aaaCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCACATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTa                  >  2:624339/1‑139 (MQ=255)

                                                                                                                      aCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTAtt                >  2:83995/1‑139 (MQ=255)

                                                                                                                      aCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTAtt                <  1:223036/139‑1 (MQ=255)

                                                                                                                       ccGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGTACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTc               >  2:278453/1‑139 (MQ=255)

                                                                                                                          gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCAc                                                                                            >  2:375925/1‑59 (MQ=255)

                                                                                                                          gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCAc                                                                                            <  1:375925/59‑1 (MQ=255)

                                                                                                                          gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatgatg                                                                         >  2:101720/1‑78 (MQ=255)

                                                                                                                          gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatgatg                                                                         <  1:101720/78‑1 (MQ=255)

                                                                                                                          gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGc            >  2:255039/1‑139 (MQ=255)

                                                                                                                                  tctGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGTACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg    <  1:278453/139‑1 (MQ=255)

                                                                                                                                  tctGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg    >  2:393220/1‑139 (MQ=255)

                                                                                                                                  tctGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg    <  2:320538/139‑1 (MQ=255)

                                                                                                                                  tctGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg    >  1:291713/1‑139 (MQ=255)

                                                                                                                                    tGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCt  >  2:242087/1‑139 (MQ=255)

GTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCT  >  NC_000913/2902407‑2902677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A