Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,815,863 |
C→T |
pseudogene (19/48 nt) |
rph ← |
ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,815,863 | 0 | C | T | 100.0%
| 95.2
/ NA
| 30 | pseudogene (19/48 nt) | rph | ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/16); total (14/16) |
ATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCA > NC_000913/3815767‑3815978
|
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGa > 1:302295/1‑139 (MQ=255)
cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAg < 1:1050273/139‑1 (MQ=255)
tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGct > 2:120757/1‑139 (MQ=255)
ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt < 1:989705/96‑1 (MQ=255)
ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt > 2:989705/1‑96 (MQ=255)
ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAGATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgat > 2:724824/1‑88 (MQ=255)
ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAGATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgat < 1:724824/88‑1 (MQ=255)
tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCtt > 1:179744/1‑123 (MQ=255)
tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCtt < 2:179744/123‑1 (MQ=255)
cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgat > 2:217909/1‑81 (MQ=255)
cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgat < 1:217909/81‑1 (MQ=255)
aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCaga < 1:844951/94‑1 (MQ=255)
aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCaga > 2:844951/1‑94 (MQ=255)
cAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg < 1:935260/139‑1 (MQ=255)
aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgat > 1:889706/1‑78 (MQ=255)
aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgat < 2:889706/78‑1 (MQ=255)
gACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa < 1:120757/139‑1 (MQ=255)
aCTCTTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTTCAATGgattcga < 1:566109/65‑1 (MQ=255)
aCTCATCAGTCGCCTTAAAAATCAGTTTGGCAGCGCCGCCTTCTGCGTCGTTACAATGgattcga > 2:566109/1‑65 (MQ=255)
gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc < 2:892434/105‑1 (MQ=255)
gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc > 1:892434/1‑105 (MQ=255)
ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg > 1:985717/1‑124 (MQ=255)
ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg < 2:985717/124‑1 (MQ=255)
aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGcc > 2:152392/1‑60 (MQ=255)
aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGcc < 1:152392/60‑1 (MQ=255)
aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg < 2:337932/113‑1 (MQ=255)
aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg > 1:337932/1‑113 (MQ=255)
ttGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca < 2:302295/139‑1 (MQ=255)
ccTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg < 2:434703/88‑1 (MQ=255)
ccTTCTGCGTCGTTACAATGGATTCGATTCCCCTC‑GGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg > 1:434703/1‑88 (MQ=255)
|
ATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCA > NC_000913/3815767‑3815978
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
