Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,609	G→A	196 (TAG→TAA)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,609	0	G	A	100.0%	102.5 / NA	32	196 (TAG→TAA)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/15); total (17/15)

TAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCA  >  NC_000913/3898515‑3898717

tAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                   >  2:960631/1‑122 (MQ=255)

tAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                   <  1:960631/122‑1 (MQ=255)

  gCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                   <  2:345270/120‑1 (MQ=255)

  gCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                   >  1:345270/1‑120 (MQ=255)

   cTAAATGCCTGGAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCTGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGc                                                                                 <  2:732377/121‑1 (MQ=255)

   cTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGACCGGAGc                                                                                 >  1:732377/1‑121 (MQ=255)

                      tggtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                         <  2:731886/110‑1 (MQ=255)

                      tggtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                         >  1:731886/1‑110 (MQ=255)

                        gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                          >  1:1044869/1‑139 (MQ=255)

                        gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                          >  2:159036/1‑139 (MQ=255)

                        gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                          <  2:1044869/139‑1 (MQ=255)

                        gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                          <  1:159036/139‑1 (MQ=255)

                             tGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                      <  2:86974/74‑1 (MQ=255)

                             tGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                      >  1:86974/1‑74 (MQ=255)

                              gTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCgg                                                                <  1:524012/111‑1 (MQ=255)

                              gTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCgg                                                                >  2:524012/1‑111 (MQ=255)

                                  gggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGaa                                                                                            >  2:341912/1‑79 (MQ=255)

                                  gggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGaa                                                                                            <  1:341912/79‑1 (MQ=255)

                                       aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                     <  1:670090/113‑1 (MQ=255)

                                       aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                     >  2:670090/1‑113 (MQ=255)

                                                   gTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                       <  1:532045/99‑1 (MQ=255)

                                                   gTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                       >  2:532045/1‑99 (MQ=255)

                                                    tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt              >  1:525619/1‑139 (MQ=255)

                                                           tGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGTCCTTATTTCTTAAGCTTAAc                                            >  2:26920/1‑102 (MQ=255)

                                                           tGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAAc                                            <  1:26920/102‑1 (MQ=255)

                                                            gCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCt                                    >  2:85453/1‑109 (MQ=255)

                                                            gCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCt                                    <  1:85453/109‑1 (MQ=255)

                                                                cAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                       >  2:455904/1‑86 (MQ=255)

                                                                cAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                       <  1:455904/86‑1 (MQ=255)

                                                                cAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCa  >  2:774712/1‑139 (MQ=255)

                                                                             tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg           <  2:1008419/117‑1 (MQ=255)

                                                                             tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg           >  1:1008419/1‑117 (MQ=255)

TAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCA  >  NC_000913/3898515‑3898717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A