Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,609	G→A	196 (TAG→TAA)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,609	0	G	A	100.0%	176.2 / NA	55	196 (TAG→TAA)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/28); total (27/28)

AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC  >  NC_000913/3898479‑3898734

aagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  1:1124809/139‑1 (MQ=255)

aagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  1:1299628/139‑1 (MQ=255)

   aTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaata                                                                                                                    >  2:44931/1‑139 (MQ=255)

     tgtgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaataat                                                                                                                  >  2:782165/1‑139 (MQ=255)

     tgtgAGAATTTCGGTATTAGAAATTCTGAACTAGCTAAATGCCTGAAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGCGTGATTTTTCGTAATTATTTAaataat                                                                                                                  >  1:789180/1‑139 (MQ=255)

       tgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATga                                                                                                                >  1:222656/1‑139 (MQ=255)

                 ggTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAGATAATGAGAACAGGCCg                                                                                                      <  1:782165/139‑1 (MQ=255)

                 ggTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                      >  1:678119/1‑139 (MQ=255)

                                  tCTAGCTAACTGCCTGGAAAAGGGTGGTGATTGTCAGGGTTAGCTCTTCCGCTGTCTCCACTGCGGCAAGCTGGGACGGTGGGGTGATTTTTCGTAATTAGTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACAt                                                                                     <  1:937424/139‑1 (MQ=255)

                                    tAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATcc                                                                                   <  2:222656/139‑1 (MQ=255)

                                    tAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATcc                                                                                   <  2:678119/139‑1 (MQ=255)

                                         aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          <  2:1134212/127‑1 (MQ=255)

                                         aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          >  1:1134212/1‑127 (MQ=255)

                                         aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCt                                                                              <  2:734420/139‑1 (MQ=255)

                                           aTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTa                                                                            >  2:614173/1‑139 (MQ=255)

                                               cTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                                        <  1:475275/139‑1 (MQ=255)

                                                tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          <  1:415123/120‑1 (MQ=255)

                                                tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          >  2:415123/1‑120 (MQ=255)

                                                tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                       >  2:1206413/1‑139 (MQ=255)

                                                tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                       <  1:1208053/139‑1 (MQ=255)

                                                tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                       >  2:440836/1‑139 (MQ=255)

                                                          tggtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCg                                                                                  <  2:1212937/118‑1 (MQ=255)

                                                          tggtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCg                                                                                  >  1:1212937/1‑118 (MQ=255)

                                                            gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  1:440836/139‑1 (MQ=255)

                                                                           aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTa                                                                                                                            <  1:51093/59‑1 (MQ=255)

                                                                           aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTa                                                                                                                            >  2:51093/1‑59 (MQ=255)

                                                                           aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCg                                            >  1:198586/1‑139 (MQ=255)

                                                                                     cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       >  1:577623/1‑54 (MQ=255)

                                                                                     cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  2:577623/54‑1 (MQ=255)

                                                                                     cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                                      <  1:957656/103‑1 (MQ=255)

                                                                                     cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                                      >  2:957656/1‑103 (MQ=255)

                                                                                     cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCtt                                  >  2:986423/1‑139 (MQ=255)

                                                                                        tctcCACTGCGGCAAGCTGAGACTGTTGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                               <  1:986423/139‑1 (MQ=255)

                                                                                        tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGc                                                                                                  <  2:204781/72‑1 (MQ=255)

                                                                                        tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGc                                                                                                  >  1:204781/1‑72 (MQ=255)

                                                                                        tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          >  1:421104/1‑80 (MQ=255)

                                                                                        tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          <  2:421104/80‑1 (MQ=255)

                                                                                        tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                               <  1:237211/139‑1 (MQ=255)

                                                                                          tcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                             >  2:937449/1‑139 (MQ=255)

                                                                                           ccACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg                            <  1:614173/139‑1 (MQ=255)

                                                                                                  ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  2:538902/41‑1 (MQ=255)

                                                                                                  ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       >  1:538902/1‑41 (MQ=255)

                                                                                                                 tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCt                                                                              >  1:317499/1‑67 (MQ=255)

                                                                                                                 tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCt                                                                              <  2:317499/67‑1 (MQ=255)

                                                                                                                 tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           >  2:377178/1‑86 (MQ=255)

                                                                                                                 tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  1:377178/86‑1 (MQ=255)

                                                                                                                 tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGc                                    >  2:1058052/1‑109 (MQ=255)

                                                                                                                 tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGc                                    <  1:1058052/109‑1 (MQ=255)

                                                                                                                  ggggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  2:808969/85‑1 (MQ=255)

                                                                                                                  ggggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           >  1:808969/1‑85 (MQ=255)

                                                                                                                   gggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  1:576886/84‑1 (MQ=255)

                                                                                                                   gggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           >  2:576886/1‑84 (MQ=255)

                                                                                                                     gTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGc  >  1:683860/1‑139 (MQ=255)

                                                                                                                           tttCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCg                                      <  1:493475/97‑1 (MQ=255)

                                                                                                                           tttCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCg                                      >  2:493475/1‑97 (MQ=255)

AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC  >  NC_000913/3898479‑3898734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A