Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,373	C→T	578 (TAG→TAA)	recJ ←	ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,373	0	C	T	100.0%	72.6 / NA	24	578 (TAG→TAA)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/11); total (13/11)

GGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTAAAAG  >  NC_000913/3036243‑3036506

ggTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                               <  1:307096/139‑1 (MQ=255)

   ccTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCa                                                                                                                            >  1:391329/1‑139 (MQ=255)

                        gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGt                                                                                                                    >  2:491742/1‑126 (MQ=255)

                        gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGt                                                                                                                    <  1:491742/126‑1 (MQ=255)

                                        ggTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCcg                                                                                       >  1:891604/1‑139 (MQ=255)

                                                    tttATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTg                                                                           >  1:763518/1‑139 (MQ=255)

                                                                     aaTGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGc                                                          <  2:943938/139‑1 (MQ=255)

                                                                       tGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGcc                                                                                                                             >  1:216559/1‑70 (MQ=255)

                                                                       tGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGcc                                                                                                                             <  2:216559/70‑1 (MQ=255)

                                                                                   agCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTcgc                                            >  2:873489/1‑139 (MQ=255)

                                                                                      gggATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa                                         <  2:763518/139‑1 (MQ=255)

                                                                                             tACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGtt                                  >  2:1096818/1‑139 (MQ=255)

                                                                                               cccAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTAt                                <  1:873489/139‑1 (MQ=255)

                                                                                                  aaTCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                                   <  1:589619/37‑1 (MQ=255)

                                                                                                  aaTCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                                   >  2:589619/1‑37 (MQ=255)

                                                                                                    tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                                  <  1:41173/84‑1 (MQ=255)

                                                                                                    tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                                  >  2:41173/1‑84 (MQ=255)

                                                                                                       gcGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                                  >  1:683206/2‑81 (MQ=255)

                                                                                                       aCGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                                  <  2:683206/81‑1 (MQ=255)

                                                                                                            ctTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGc                   >  2:514430/1‑139 (MQ=255)

                                                                                                              tttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCgg                 >  2:626019/1‑139 (MQ=255)

                                                                                                               ttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGc                           >  2:618862/1‑128 (MQ=255)

                                                                                                               ttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGc                           <  1:618862/128‑1 (MQ=255)

                                                                                                                             tGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTAAAAg  <  2:326045/139‑1 (MQ=255)

GGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTAAAAG  >  NC_000913/3036243‑3036506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A