Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,373	C→T	578 (TAG→TAA)	recJ ←	ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,373	0	C	T	100.0%	171.7 / NA	54	578 (TAG→TAA)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/23); total (31/23)

GTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTAAAAG  >  NC_000913/3036244‑3036506

gTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGc                                                                                                                              <  1:900054/139‑1 (MQ=255)

    tGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGa                                                                                                                          >  1:15035/1‑139 (MQ=255)

           gATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTc                                                                                                                   >  2:794519/1‑139 (MQ=255)

           gATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTc                                                                                                                   >  2:269905/1‑139 (MQ=255)

           gATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTc                                                                                                                   >  2:207388/1‑139 (MQ=255)

                       gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                               >  2:768840/1‑115 (MQ=255)

                       gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                               <  1:768840/115‑1 (MQ=255)

                       gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTg                                                                                                       >  2:530993/1‑139 (MQ=255)

                       gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTg                                                                                                       >  1:1036579/1‑139 (MQ=255)

                        aTTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGc                                                                                                      >  1:710360/1‑139 (MQ=255)

                                  aaCATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGt                                                                                            >  2:1169449/1‑139 (MQ=255)

                                  aaCATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTCTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGt                                                                                            >  1:879233/1‑139 (MQ=255)

                                   aCATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGtt                                                                                           <  1:269905/139‑1 (MQ=255)

                                       ggTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCgatg                                                                                                               >  2:327963/1‑115 (MQ=255)

                                       ggTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCgatg                                                                                                               <  1:327963/115‑1 (MQ=255)

                                       ggTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCcg                                                                                       >  2:591860/1‑139 (MQ=255)

                                        gTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                                   <  1:944006/94‑1 (MQ=255)

                                        gTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                                   >  2:944006/1‑94 (MQ=255)

                                             aTTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                               <  1:982981/93‑1 (MQ=255)

                                             aTTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                               >  2:982981/1‑93 (MQ=255)

                                                       ttGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                                  >  1:809510/1‑128 (MQ=255)

                                                       ttGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                                  <  2:809510/128‑1 (MQ=255)

                                                            cTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGt                                                                                            <  1:474093/113‑1 (MQ=255)

                                                            cTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGt                                                                                            >  2:474093/1‑113 (MQ=255)

                                                               gTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTta                                                               <  1:1169449/139‑1 (MQ=255)

                                                                         ggTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGtt                                                     >  2:431397/1‑139 (MQ=255)

                                                                          gTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                                   >  1:324013/1‑60 (MQ=255)

                                                                          gTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                                   <  2:324013/60‑1 (MQ=255)

                                                                                    cGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                          >  1:54560/1‑139 (MQ=255)

                                                                                     gggATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa                                         <  2:879233/139‑1 (MQ=255)

                                                                                       gATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACg                                       >  1:934222/1‑139 (MQ=255)

                                                                                         ttGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGcc                                     <  2:607539/139‑1 (MQ=255)

                                                                                           gTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGt                                   <  2:1061191/139‑1 (MQ=255)

                                                                                            tACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                    <  2:708308/105‑1 (MQ=255)

                                                                                            tACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                    >  1:708308/1‑105 (MQ=255)

                                                                                             aCCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGa                                                                                    <  2:472230/88‑1 (MQ=255)

                                                                                             aCCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGa                                                                                    >  1:472230/1‑88 (MQ=255)

                                                                                               ccAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATc                               <  1:530993/139‑1 (MQ=255)

                                                                                                 aaTCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGata                                                                                                                        <  2:1082998/48‑1 (MQ=255)

                                                                                                 aaTCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGata                                                                                                                        >  1:1082998/1‑48 (MQ=255)

                                                                                                 aaTCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCg                             >  1:1270136/1‑139 (MQ=255)

                                                                                                  aTCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                          >  2:796617/1‑125 (MQ=255)

                                                                                                  aTCCACGATCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATCATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                          <  1:796617/125‑1 (MQ=255)

                                                                                                   tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGACGTTAtaccgc                           <  1:794519/139‑6 (MQ=255)

                                                                                                     cACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCa                         <  2:710360/139‑1 (MQ=255)

                                                                                                             tttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACt                                                                                >  2:922258/1‑76 (MQ=255)

                                                                                                             tttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACt                                                                                <  1:922258/76‑1 (MQ=255)

                                                                                                                      agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACAt        >  2:95989/1‑139 (MQ=255)

                                                                                                                      agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACAt        >  1:842869/1‑139 (MQ=255)

                                                                                                                        aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTa      >  2:770812/1‑139 (MQ=255)

                                                                                                                           aTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTaaaa   >  2:710206/1‑139 (MQ=255)

                                                                                                                            tGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTAAAAg  <  1:95989/139‑1 (MQ=255)

                                                                                                                             gACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGt                                   >  2:1047003/1‑105 (MQ=255)

                                                                                                                             gACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGt                                   <  1:1047003/105‑1 (MQ=255)

GTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTAAAAG  >  NC_000913/3036244‑3036506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A