Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 781 46.9 2231650 93.5% 2086592 109.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,240,984 (G)7→8 coding (1041/1245 nt) sstT → sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,9771.G100.0% 138.6 / NA 39A345G (GCG→GGG) sstTsodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/21);  total (18/21)

GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAC  >  NC_000913/3240844‑3241107
                                                                                                                                      |                                                                                                                                  
gcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggg                                                                                                                               >  2:548739/1‑139 (MQ=255)
  gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggg                                                                                                                             <  2:499419/139‑1 (MQ=255)
    gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           <  1:901741/139‑3 (MQ=255)
    gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           >  1:486085/1‑137 (MQ=255)
        agcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTctct                                                                                                                        <  1:548739/139‑1 (MQ=255)
         gcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCAGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctg                                                                                                                       <  2:886/139‑1 (MQ=255)
                    tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATccc                                                                                                            >  1:298499/1‑139 (MQ=255)
                       cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         <  2:486085/139‑1 (MQ=255)
                         tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         >  1:315844/1‑137 (MQ=255)
                         tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         <  2:315844/137‑1 (MQ=255)
                         tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                       >  2:327827/1‑139 (MQ=255)
                         tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                       >  2:336503/1‑139 (MQ=255)
                           ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                                                     >  2:61292/1‑139 (MQ=255)
                           ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTAGcc                                                                                                     >  2:405917/1‑139 (MQ=255)
                           ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           >  2:223905/1‑114 (MQ=255)
                           ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           <  1:223905/116‑3 (MQ=255)
                               cGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                                 <  1:336503/139‑1 (MQ=255)
                                   ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                <  1:635508/103‑1 (MQ=255)
                                   ggctgCGGTTAATACGCAGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                >  2:635508/1‑103 (MQ=255)
                                      tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGtt                                                                                          <  1:327827/139‑1 (MQ=255)
                                        cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      >  2:42924/1‑125 (MQ=255)
                                        cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      <  1:42924/125‑1 (MQ=255)
                                          gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           >  2:994119/1‑99 (MQ=255)
                                          gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           <  1:994119/101‑3 (MQ=255)
                                                    tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      >  2:124698/1‑113 (MQ=255)
                                                    tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      <  1:124698/113‑1 (MQ=255)
                                                     gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  2:590767/1‑139 (MQ=255)
                                                     gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  2:452909/1‑139 (MQ=255)
                                                     gggTATTCCGGCCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           <  1:722071/139‑1 (MQ=255)
                                                          ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                                                                                                                   >  1:941082/1‑77 (MQ=255)
                                                          ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                                                                                                                   <  2:941082/77‑1 (MQ=255)
                                                                  gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                              >  2:213847/1‑139 (MQ=255)
                                                                              gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      >  2:47112/1‑87 (MQ=255)
                                                                              gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      <  1:47112/87‑1 (MQ=255)
                                                                                  tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                                                         <  1:350097/96‑1 (MQ=255)
                                                                                  tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                                                         >  2:350097/1‑96 (MQ=255)
                                                                                    ctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCttt                                            >  2:482355/1‑139 (MQ=255)
                                                                                                 tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           <  2:771664/95‑1 (MQ=255)
                                                                                                 tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  1:771664/1‑95 (MQ=255)
                                                                                                     ttCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCa                           <  1:55960/139‑1 (MQ=255)
                                                                                                         ctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGAc                       <  2:915474/139‑1 (MQ=255)
                                                                                                                    tgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           <  2:165658/76‑1 (MQ=255)
                                                                                                                    tgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  1:165658/1‑76 (MQ=255)
                                                                                                                     ggCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAAcc           <  2:461947/139‑1 (MQ=255)
                                                                                                                      gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         >  2:44500/1‑44 (MQ=38)
                                                                                                                      gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         <  1:44500/44‑1 (MQ=255)
                                                                                                                      gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGt                                                                                                  >  1:713908/1‑51 (MQ=255)
                                                                                                                      gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGt                                                                                                  <  2:713908/51‑1 (MQ=255)
                                                                                                                           tCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCTGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg     <  1:590767/139‑1 (MQ=255)
                                                                                                                           tCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg     <  1:452909/139‑1 (MQ=255)
                                                                                                                              ggCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAc  <  1:213847/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAC  >  NC_000913/3240844‑3241107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A