Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)_7→8	coding (1041/1245 nt)	sstT →	sodium:serine/threonine symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	93.8%	271.7 / 8.4	80	coding (1034/1245 nt)	sstT	sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (38/37); total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTC  >  NC_000913/3240843‑3241112

ggcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g                                                                                                                                      <  1:1338153/139‑1 (MQ=255)

 gcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTTAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑g                                                                                                                                    >  2:1687946/1‑139 (MQ=255)

 gcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTTAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑g                                                                                                                                    >  1:1749112/1‑139 (MQ=255)

 gcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑g                                                                                                                                    >  2:1385304/1‑139 (MQ=255)

   gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggg                                                                                                                                  <  1:1101893/139‑1 (MQ=255)

   gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggg                                                                                                                                  <  2:694737/139‑1 (MQ=255)

     gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggt                                                                                                                                >  1:1648232/1‑137 (MQ=255)

     gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggt                                                                                                                                <  1:1687946/139‑3 (MQ=255)

        cagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggtctc                                                                                                                             <  1:751079/139‑6 (MQ=255)

          gcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTctg                                                                                                                            <  1:1334792/139‑1 (MQ=255)

          gcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTctg                                                                                                                            <  1:1658761/139‑1 (MQ=255)

             aTCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTctgctg                                                                                                                         <  2:1648232/139‑1 (MQ=255)

                 cTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGa                                                                                                                     <  1:1468740/139‑1 (MQ=255)

                   aTTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggt                                                                                                                                <  2:1110414/125‑3 (MQ=255)

                   aTTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggt                                                                                                                                >  1:1110414/1‑123 (MQ=255)

                     tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATccc                                                                                                                 >  1:1619145/1‑139 (MQ=255)

                      aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCa                                                                                                                <  1:1452711/139‑1 (MQ=255)

                        cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              <  1:710216/139‑1 (MQ=255)

                         gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGTTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g                                                                                                                                      >  1:1284860/1‑114 (MQ=255)

                         gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g                                                                                                                                      <  2:1284860/114‑1 (MQ=255)

                          tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTgg                                                                                                            <  2:1311842/139‑1 (MQ=255)

                           gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           <  1:383895/139‑1 (MQ=255)

                           gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           <  1:949363/139‑1 (MQ=255)

                            ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              <  1:72946/135‑1 (MQ=255)

                            ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              >  2:72946/1‑135 (MQ=255)

                                     gctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                                      >  2:1379094/1‑133 (MQ=255)

                                     gctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                                      <  1:1379094/133‑1 (MQ=255)

                                      ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGt                                                                                                >  2:1598035/1‑139 (MQ=255)

                                      ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              >  2:982759/1‑124 (MQ=255)

                                      ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              <  1:982759/124‑1 (MQ=255)

                                               aTACGATGGGTATTCCGGTCGATTTGCCGACGGCGCTGCTGTTGAGCGTGGAGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGCG‑GGGTCCCTGCTGCTGTTCCCACTGGCCTGTAATATGTTCGGTAttt                                                                                       >  1:1737487/1‑139 (MQ=255)

                                                     tGGGTATTCCGGTCGATCTGCCCCCGGCGCTGCTGTTGAGCGTGGTGGCTTCTTGGTGTGCCTGTGGCGCATCCGGCTTGGGGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTG‑AATATGTTCGGTATTTCGAACGa                                                                                <  2:1456877/139‑1 (MQ=255)

                                                     tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTg                                                                                                             >  1:198723/1‑111 (MQ=255)

                                                     tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTg                                                                                                             <  2:198723/111‑1 (MQ=255)

                                                     tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACg                                                                                 >  2:626687/1‑139 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTgg                                                                                                            >  2:520187/1‑111 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTgg                                                                                                            <  1:520187/111‑1 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           >  2:984524/1‑112 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           >  2:348359/1‑112 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           <  1:984524/112‑1 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           <  1:348359/112‑1 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                                >  1:1468812/1‑139 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                                >  1:735088/1‑139 (MQ=255)

                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGCGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                                >  1:294509/1‑139 (MQ=255)

                                                        gTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                              <  2:1749112/139‑1 (MQ=255)

                                                        gTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                              >  2:379013/1‑139 (MQ=255)

                                                         tATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGatat                                                                             <  2:1468812/139‑1 (MQ=255)

                                                           ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCg                                                                           <  2:1701822/139‑1 (MQ=255)

                                                            tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                                                          >  1:1643678/1‑139 (MQ=255)

                                                            tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                                                          >  1:1348556/1‑139 (MQ=255)

                                                                 tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTgg                                                                                                            <  1:716561/100‑1 (MQ=255)

                                                                 tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTgg                                                                                                            >  2:716561/1‑100 (MQ=255)

                                                                 tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGc                                                                     >  2:1066483/1‑139 (MQ=255)

                                                                   gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                                   >  1:1464579/1‑139 (MQ=255)

                                                                      cTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggtctc                                                                                                                             <  1:1234756/77‑6 (MQ=255)

                                                                      cTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggtctc                                                                                                                             >  2:1234756/1‑72 (MQ=255)

                                                                           cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAat                                                                                                    <  2:762004/98‑1 (MQ=255)

                                                                           cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAat                                                                                                    >  1:762004/1‑98 (MQ=255)

                                                                              ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              <  1:667903/85‑1 (MQ=255)

                                                                              ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              >  2:667903/1‑85 (MQ=255)

                                                                              ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                        >  2:90620/1‑139 (MQ=255)

                                                                              ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                        <  1:1066483/139‑1 (MQ=255)

                                                                                 gctgctGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           <  2:218118/85‑1 (MQ=255)

                                                                                 gctgctGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           >  1:218118/1‑85 (MQ=255)

                                                                                   tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt                                                   >  1:1504732/1‑139 (MQ=255)

                                                                                         tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatca                                             <  2:1348556/139‑1 (MQ=255)

                                                                                          gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGCTTGCCGTCGGCTTTatcat                                            <  2:1198871/139‑1 (MQ=255)

                                                                                               tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTg                                                                                                        <  2:1274683/74‑1 (MQ=255)

                                                                                               tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTg                                                                                                        >  1:1274683/1‑74 (MQ=255)

                                                                                               tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                       <  2:1464579/139‑1 (MQ=255)

                                                                                               tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                       <  2:1643678/139‑1 (MQ=255)

                                                                                               tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggt                                                                                                                                <  1:1347473/49‑3 (MQ=255)

                                                                                               tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg‑g‑ggggt                                                                                                                                >  2:1347473/1‑47 (MQ=255)

                                                                                                    gCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTg                                  >  1:1404773/1‑139 (MQ=255)

                                                                                                            gtgtgCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGActc                          >  2:345516/1‑139 (MQ=255)

                                                                                                               tgCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           <  1:410225/55‑1 (MQ=255)

                                                                                                               tgCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           >  2:410225/1‑55 (MQ=255)

                                                                                                               tgCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                       <  1:204846/123‑1 (MQ=255)

                                                                                                               tgCCTGTGGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                       >  2:204846/1‑123 (MQ=255)

                                                                                                                   tgtgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           <  1:477874/51‑1 (MQ=255)

                                                                                                                   tgtgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGc                                                                                                           >  2:477874/1‑51 (MQ=255)

                                                                                                                   tgtgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaa                   >  1:677857/1‑139 (MQ=255)

                                                                                                                   tgtgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCACGCCGGGGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaa                   >  1:1534334/1‑139 (MQ=255)

                                                                                                                     tgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                                                         <  1:1423329/99‑1 (MQ=255)

                                                                                                                     tgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                                                         >  2:1423329/1‑99 (MQ=255)

                                                                                                                     tgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat                                            >  2:36613/1‑112 (MQ=255)

                                                                                                                     tgGCGCATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat                                            <  1:36613/112‑1 (MQ=255)

                                                                                                                         gcATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                        >  1:959734/1‑96 (MQ=255)

                                                                                                                         gcATCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                        <  2:959734/96‑1 (MQ=255)

                                                                                                                            tCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              >  2:607089/1‑39 (MQ=255)

                                                                                                                            tCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACt                                                                                                              <  1:607089/39‑1 (MQ=255)

                                                                                                                            tCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg                                                                                             >  2:1145878/1‑56 (MQ=255)

                                                                                                                            tCCGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg                                                                                             <  1:1145878/56‑1 (MQ=255)

                                                                                                                             ccGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGa         <  1:90620/139‑1 (MQ=255)

                                                                                                                             ccGGCGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGa         >  2:194712/1‑139 (MQ=255)

                                                                                                                                 cGTGGCGGGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAActc     >  1:1253970/1‑139 (MQ=255)

                                                                                                                                 cGTGGCGGGG‑G‑GGGTCTCTGCTGCTGACCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAActc     >  1:916205/1‑139 (MQ=255)

                                                                                                                                    ggC‑GGG‑GTGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTc  <  2:1737487/139‑1 (MQ=255)

GGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGG‑G‑GGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTC  >  NC_000913/3240843‑3241112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A