Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,695,997	C→T	63 (TAG→TAA)	yhjR ←	DUF2629 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,695,997	0	C	T	100.0%	150.0 / NA	47	63 (TAG→TAA)	yhjR	DUF2629 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/21); total (26/21)

CATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695874‑3696132

caTCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCa                                                                                                                          >  2:1013699/1‑139 (MQ=255)

  tCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaaa                                                                                                                        <  2:313951/139‑1 (MQ=255)

          caGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaa                                                                                                                         >  2:777833/1‑130 (MQ=255)

          caGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaa                                                                                                                         <  1:777833/130‑1 (MQ=255)

            ggACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGc                                                                                                              <  2:753622/139‑1 (MQ=255)

                aTTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACg                                                                                                          >  2:424257/1‑139 (MQ=255)

                   ttCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGcc                                                                                                       <  1:424257/139‑1 (MQ=255)

                    tctcCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCCGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCa                                                                                                      >  1:1107973/1‑139 (MQ=255)

                                       aCCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   >  1:810710/1‑139 (MQ=255)

                                       aCCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   >  1:838070/1‑139 (MQ=255)

                                       aCCAGGCTAATGCAGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   >  2:71060/1‑139 (MQ=255)

                                        ccAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCa                                                                                  >  2:686757/1‑139 (MQ=255)

                                              tAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                            <  2:623759/139‑1 (MQ=255)

                                                   cggcggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTg                                                                                                                   >  1:182327/1‑95 (MQ=255)

                                                   cggcggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTg                                                                                                                   <  2:182327/95‑1 (MQ=255)

                                                      cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                                    >  1:901653/1‑139 (MQ=255)

                                                      cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                                    >  1:903278/1‑139 (MQ=255)

                                                             ggTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAACCTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGTGAAatat                                                             <  2:1107973/139‑1 (MQ=255)

                                                             ggTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAatat                                                             <  1:71060/139‑1 (MQ=255)

                                                                tgttgtCCCCACGTCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCgg                                                          >  2:719775/1‑139 (MQ=255)

                                                                 gttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                         <  2:265227/139‑1 (MQ=255)

                                                                  ttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                        <  1:299399/139‑1 (MQ=255)

                                                                       cccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATc                                                   >  1:436623/1‑139 (MQ=255)

                                                                         cACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCaa                                                 >  2:900207/1‑139 (MQ=255)

                                                                                          ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAAt                                >  2:1074059/1‑139 (MQ=255)

                                                                                                  cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCtt                        >  2:655754/1‑139 (MQ=255)

                                                                                                    cAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTa                      >  1:565195/1‑139 (MQ=255)

                                                                                                     aGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa                     <  2:810710/139‑1 (MQ=255)

                                                                                                     aGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa                     <  2:436623/139‑1 (MQ=255)

                                                                                                      gTACGGCCATTCACCAATCCGTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAAc                    >  2:653057/1‑139 (MQ=255)

                                                                                                      gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAAc                    >  2:1028595/1‑139 (MQ=255)

                                                                                                       tACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                                  >  2:656739/1‑44 (MQ=255)

                                                                                                       tACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                                  <  1:656739/44‑1 (MQ=255)

                                                                                                        aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa                     <  1:910503/136‑1 (MQ=255)

                                                                                                        aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa                     >  2:910503/1‑136 (MQ=255)

                                                                                                        aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc                  >  2:1008126/1‑139 (MQ=255)

                                                                                                            ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                         >  2:704302/1‑96 (MQ=255)

                                                                                                            ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                         <  1:704302/96‑1 (MQ=255)

                                                                                                               ttCACCAATCCCTTACTTTTGTTGCGCAGACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAata           <  1:667192/139‑1 (MQ=255)

                                                                                                                tCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAatat          <  1:1028595/139‑1 (MQ=255)

                                                                                                                 cACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc                  <  2:799589/130‑1 (MQ=255)

                                                                                                                 cACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc                  >  1:799589/1‑130 (MQ=255)

                                                                                                                    cAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCAtt      <  1:1074059/139‑1 (MQ=255)

                                                                                                                    cAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCAtt      <  1:655754/139‑1 (MQ=255)

                                                                                                                      aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                         >  2:532876/1‑86 (MQ=255)

                                                                                                                      aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                         <  1:532876/86‑1 (MQ=255)

                                                                                                                      aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCt    >  1:231822/1‑139 (MQ=255)

                                                                                                                        ccctTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg  <  2:947713/139‑1 (MQ=255)

CATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695874‑3696132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A