Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,695,997	C→T	63 (TAG→TAA)	yhjR ←	DUF2629 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,695,997	0	C	T	100.0%	130.9 / NA	42	63 (TAG→TAA)	yhjR	DUF2629 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/20); total (22/20)

TGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695862‑3696132

tGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTAc                                                                                                                                      <  2:483374/139‑1 (MQ=255)

 gTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACt                                                                                                                                     >  1:497915/1‑139 (MQ=255)

     gggCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttg                                                                                                                                 <  1:43119/139‑1 (MQ=255)

         aggcaTCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgc                                                                                                                             >  1:191893/1‑139 (MQ=255)

                 accaccAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAActc                                                                                                                     >  1:448113/1‑139 (MQ=255)

                              tttCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                        <  1:545075/139‑1 (MQ=255)

                                        cATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTa                                                                                              <  1:113361/139‑1 (MQ=255)

                                                             tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            <  2:27030/88‑1 (MQ=255)

                                                             tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            >  1:27030/1‑88 (MQ=255)

                                                             tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgc                                                                         >  1:401993/1‑139 (MQ=255)

                                                              gcggcgGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        <  1:276506/139‑1 (MQ=255)

                                                                  cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATGCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                                    >  2:545622/1‑139 (MQ=255)

                                                                  cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                                    >  1:369736/1‑139 (MQ=255)

                                                                       aTGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAat                                                               <  1:417475/139‑1 (MQ=255)

                                                                        tGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAata                                                              <  2:191893/139‑1 (MQ=255)

                                                                             gttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACATTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                         <  2:552296/139‑1 (MQ=255)

                                                                               tgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        >  2:88635/1‑122 (MQ=255)

                                                                               tgtCCCCACGCCTACCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        <  1:88635/122‑1 (MQ=255)

                                                                                gtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCACTCCCTTACTTTTGTTGCCCAAACTCTGCCAGCAACGGCCAGCGTTGTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATa                                                      >  2:450937/1‑139 (MQ=255)

                                                                                 tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAata                                                              <  1:46710/130‑1 (MQ=255)

                                                                                 tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAata                                                              >  2:46710/1‑130 (MQ=255)

                                                                                 tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATaa                                                     >  1:759816/1‑139 (MQ=255)

                                                                                  ccccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAAt                                                    <  2:448113/139‑1 (MQ=255)

                                                                                   cccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATc                                                   >  2:763671/1‑139 (MQ=255)

                                                                                         ccTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATc                                             <  1:586363/139‑1 (MQ=255)

                                                                                             cccGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGTGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGc                                         >  1:208355/1‑139 (MQ=255)

                                                                                                      ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   <  2:33881/88‑1 (MQ=255)

                                                                                                      ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   >  1:33881/1‑88 (MQ=255)

                                                                                                       cTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATg                               >  2:124542/1‑139 (MQ=255)

                                                                                                       cTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATg                               >  1:338258/1‑139 (MQ=255)

                                                                                                       cTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATg                               <  2:576472/139‑1 (MQ=255)

                                                                                                        tGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGc                              >  1:551952/1‑139 (MQ=255)

                                                                                                              cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        <  1:405255/91‑1 (MQ=255)

                                                                                                              cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        >  2:405255/1‑91 (MQ=255)

                                                                                                                    aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc                  >  1:136788/1‑139 (MQ=255)

                                                                                                                      ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca                <  2:369736/139‑1 (MQ=255)

                                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                            >  1:27334/1‑77 (MQ=255)

                                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                            <  2:27334/77‑1 (MQ=255)

                                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAata           >  1:271132/1‑139 (MQ=255)

                                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAata           >  1:102088/1‑139 (MQ=255)

                                                                                                                              aCCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGGCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCa        <  2:551952/139‑1 (MQ=255)

                                                                                                                                cAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCAtt      <  2:102088/139‑1 (MQ=255)

                                                                                                                                    ccctTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg  <  1:124542/139‑1 (MQ=255)

TGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695862‑3696132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A