Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,789,913 |
C→T |
A57T (GCA→ACA) |
waaH ← |
LPS(HepIII)‑glucuronic acid glycosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,789,913 | 0 | C | T | 100.0%
| 74.5
/ NA
| 25 | A57T (GCA→ACA) | waaH | LPS(HepIII)‑glucuronic acid glycosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/15); total (10/15) |
GGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAA > NC_000913/3789783‑3790042
|
ggATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACt > 2:67361/1‑139 (MQ=255)
ggATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCAGTGTAATACt > 2:917592/1‑139 (MQ=255)
gATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACtt > 2:218391/1‑139 (MQ=255)
aCTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGc > 2:124577/1‑137 (MQ=255)
aCTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGc < 1:124577/137‑1 (MQ=255)
tCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCaa > 1:9124/1‑139 (MQ=255)
gTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACa < 2:9124/139‑1 (MQ=255)
cATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAAACCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAAtt < 1:915533/139‑1 (MQ=255)
cGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATc < 2:13174/139‑1 (MQ=255)
aCATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCAt > 2:24709/1‑139 (MQ=255)
tGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATg < 1:67361/139‑1 (MQ=255)
aaTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCaga < 1:917592/139‑1 (MQ=255)
aTCCCACGATTACGCGCCACCGATGCGCCAGCATTTGCATGATGCAACAAACCAACGTGCGTATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAAATATCCGTTGAACCATCGTTAATAATAATGATGTCCagag < 1:378472/139‑1 (MQ=255)
aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag > 1:415720/1‑139 (MQ=255)
aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag < 2:415720/139‑1 (MQ=255)
aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag < 1:359/139‑1 (MQ=255)
cGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTcc > 2:400323/1‑139 (MQ=255)
gcgcCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTg < 1:863628/139‑1 (MQ=255)
gcCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCaa < 1:694470/79‑1 (MQ=255)
gcCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCaa > 2:694470/1‑79 (MQ=255)
cgcCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGa < 1:400323/139‑1 (MQ=255)
ccAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAAtt > 1:86285/1‑74 (MQ=255)
ccAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAAtt < 2:86285/74‑1 (MQ=255)
cgaacgTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGc < 1:218391/139‑1 (MQ=255)
aGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATaa < 2:782036/139‑1 (MQ=255)
|
GGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAA > NC_000913/3789783‑3790042
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
