Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,876,944	G→A	A122T (GCC→ACC)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,876,944	0	G	A	100.0%	136.7 / NA	43	A122T (GCC→ACC)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/20); total (23/20)

TAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACC  >  NC_000913/2876811‑2877069

tAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCg                                                                                                                          <  1:1064405/139‑1 (MQ=255)

     gTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAg                                                                                                                     >  2:454900/1‑139 (MQ=255)

                      gcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATg                                                                                                    >  1:580816/1‑139 (MQ=255)

                       cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                   >  1:1106508/1‑139 (MQ=255)

                       cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                   >  2:162394/1‑139 (MQ=255)

                       cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATAGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                   >  1:1263539/1‑139 (MQ=255)

                                            ggCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGtt                                                                              >  2:1367124/1‑139 (MQ=255)

                                             gCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTa                                                                             >  2:1361697/1‑139 (MQ=255)

                                              cATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTAc                                                                            <  1:798356/139‑1 (MQ=255)

                                               aTAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACa                                                                           <  2:1106508/139‑1 (MQ=255)

                                                   cGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATAGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                       <  2:1263539/139‑1 (MQ=255)

                                                       aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATgg                                                                   >  2:847288/1‑139 (MQ=255)

                                                       aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATgg                                                                   >  1:471799/1‑139 (MQ=255)

                                                                    tGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccgc                                                      <  1:725411/139‑1 (MQ=255)

                                                                         gCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                                   <  1:1105052/105‑1 (MQ=255)

                                                                         gCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                                   >  2:1105052/1‑105 (MQ=255)

                                                                                    aGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                      <  1:686066/139‑1 (MQ=255)

                                                                                          aGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGaa                                >  1:825113/1‑139 (MQ=255)

                                                                                                   gcagATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGATGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGaa                       <  1:925264/139‑1 (MQ=255)

                                                                                                              ttATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGaaaaa            >  2:41684/1‑139 (MQ=255)

                                                                                                                 tGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc         >  1:323305/1‑139 (MQ=255)

                                                                                                                  ggCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACg        <  1:553515/139‑1 (MQ=255)

                                                                                                                  ggCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACg        >  1:100877/1‑139 (MQ=255)

                                                                                                                   gcgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGc       >  2:1178439/1‑139 (MQ=255)

                                                                                                                   gcgcATCTGGATACTTACACCACGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGAAGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGACGGAAAAATACGc       >  1:369703/1‑139 (MQ=255)

                                                                                                                     gcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCt     <  1:847288/139‑1 (MQ=255)

                                                                                                                     gcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCt     <  2:471799/139‑1 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                       <  2:618494/72‑1 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                       >  1:618494/1‑72 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCt     >  2:1258845/1‑138 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCt     <  1:1258845/138‑1 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa    <  1:162394/139‑1 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa    <  1:1367124/139‑1 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa    <  2:323305/139‑1 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa    <  2:580816/139‑1 (MQ=255)

                                                                                                                      cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGC‑GGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTAc   >  1:582854/1‑139 (MQ=255)

                                                                                                                        tCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTAcc  >  1:357336/1‑139 (MQ=255)

                                                                                                                         cTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGc       >  1:507887/1‑133 (MQ=255)

                                                                                                                         cTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGc       <  2:507887/133‑1 (MQ=255)

                                                                                                                                cTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACCAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGCAATTGGCATaa                       <  1:792694/110‑1 (MQ=255)

                                                                                                                                cTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                                                                      <  2:837617/63‑1 (MQ=255)

                                                                                                                                cTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                                                                      >  1:837617/1‑63 (MQ=255)

                                                                                                                                cTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGaa                       >  2:792694/1‑110 (MQ=255)

TAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACC  >  NC_000913/2876811‑2877069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A