Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,876,944	G→A	A122T (GCC→ACC)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,876,944	0	G	A	100.0%	158.7 / NA	50	A122T (GCC→ACC)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/24); total (26/24)

CGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTA  >  NC_000913/2876818‑2877074

cGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCg                                                                                                                        <  2:70785/139‑1 (MQ=255)

 ggTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGa                                                                                                                       >  2:568145/1‑139 (MQ=255)

             ccgcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGa                                                                                                           >  1:96597/1‑139 (MQ=255)

             ccgcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGa                                                                                                           >  1:5992/1‑139 (MQ=255)

             ccgcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGa                                                                                                           >  1:306753/1‑139 (MQ=255)

               gcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATg                                                                                                         >  1:77282/1‑139 (MQ=255)

                cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGa                                                                                                                           <  2:395241/120‑1 (MQ=255)

                cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGa                                                                                                                           >  1:395241/1‑120 (MQ=255)

                cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                        >  1:164553/1‑139 (MQ=255)

                cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                        >  2:558059/1‑139 (MQ=255)

                cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                        >  1:464688/1‑139 (MQ=255)

                cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                        >  1:298006/1‑139 (MQ=255)

                                        aTAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACa                                                                                <  2:164553/139‑1 (MQ=255)

                                          aaCGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAg                                                                              <  1:581923/139‑1 (MQ=255)

                                            cGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                            <  2:96597/139‑1 (MQ=255)

                                            cGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                            <  2:168866/139‑1 (MQ=255)

                                             gTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                                                                           <  1:293440/139‑1 (MQ=255)

                                                aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                                     >  2:240731/1‑126 (MQ=255)

                                                aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                                     <  1:240731/126‑1 (MQ=255)

                                                aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATgg                                                                        >  1:630402/1‑139 (MQ=255)

                                                    gAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga                                                                    >  1:390287/1‑139 (MQ=255)

                                                      aGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATa                                                                  >  2:329719/1‑139 (MQ=255)

                                                             tGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccgc                                                           <  2:464688/139‑1 (MQ=255)

                                                                  gCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGtt                                                      >  2:22016/1‑139 (MQ=255)

                                                                      cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTa                                                                                  <  2:229537/107‑1 (MQ=255)

                                                                      cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTa                                                                                  >  1:229537/1‑107 (MQ=255)

                                                                      cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAgg                                                  >  1:292531/1‑139 (MQ=255)

                                                                       tCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                                     >  2:11372/1‑103 (MQ=255)

                                                                       tCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                                     <  1:11372/103‑1 (MQ=255)

                                                                        cATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                                <  1:603212/139‑1 (MQ=255)

                                                                             aGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                           <  1:329719/139‑1 (MQ=255)

                                                                               gCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCt                                         <  1:218600/139‑1 (MQ=255)

                                                                                     cgccgcAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAAtt                                   >  2:82538/1‑139 (MQ=255)

                                                                                             cagATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAAc                           <  1:558059/139‑1 (MQ=255)

                                                                                                  catcatTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACACGGAATGGATGATAACGCCGCAGGTATAGGTGTCATGCTGCAATTGGCAGAACGCCTg                      >  1:72242/1‑139 (MQ=255)

                                                                                                       ttATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGaaaaa                 >  1:397739/1‑139 (MQ=255)

                                                                                                          tGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc              >  2:54658/1‑139 (MQ=255)

                                                                                                             cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGcc           <  2:306753/139‑1 (MQ=255)

                                                                                                              gcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCt          <  1:82538/139‑1 (MQ=255)

                                                                                                              gcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCt          <  2:5992/139‑1 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                           <  2:45466/105‑1 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                           >  1:45466/1‑105 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa         <  2:504353/139‑1 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa         >  2:402215/1‑139 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa         <  2:397739/139‑1 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa         <  2:77282/139‑1 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa         <  2:316063/139‑1 (MQ=255)

                                                                                                               cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa         >  1:627093/1‑139 (MQ=255)

                                                                                                                aTCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTAc        >  2:745625/1‑139 (MQ=255)

                                                                                                                      aTACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTa  <  1:22016/139‑1 (MQ=255)

CGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTA  >  NC_000913/2876818‑2877074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A