Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,101,039 |
(C)7→8 |
coding (585/720 nt) |
yggN ← |
DUF2884 family putative periplasmic protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,101,032 | 1 | . | C | 97.1%
| 110.9
/ ‑3.2
| 34 | coding (592/720 nt) | yggN | DUF2884 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (13/20); total (13/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 |
AATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCC > NC_000913/3100895‑3101138
|
aaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c < 2:185235/139‑1 (MQ=255)
gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccc > 2:1340394/1‑139 (MQ=255)
aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTccc < 2:1281307/139‑1 (MQ=255)
aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCa < 2:664079/139‑1 (MQ=255)
tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGca < 2:1321888/139‑1 (MQ=255)
ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGcaca > 1:265852/1‑139 (MQ=255)
ggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa > 1:216976/1‑139 (MQ=255)
ggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa > 2:1013508/1‑139 (MQ=255)
tGCGGCTATCTTCCATAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg < 1:861945/114‑5 (MQ=255)
tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg > 2:861945/1‑110 (MQ=255)
tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca < 1:474143/112‑3 (MQ=255)
tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca > 2:474143/1‑110 (MQ=255)
tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 1:640546/139‑1 (MQ=255)
tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTGTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 1:1340394/139‑1 (MQ=255)
tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCtt < 2:265852/139‑1 (MQ=255)
tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTTCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACAtt < 2:1209740/111‑1 (MQ=255)
tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACAtt > 1:1209740/1‑111 (MQ=255)
tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt < 1:638604/109‑1 (MQ=255)
tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt > 2:638604/1‑109 (MQ=255)
tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 2:436060/1‑139 (MQ=255)
tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 1:1297173/1‑139 (MQ=255)
acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAcc > 2:568937/1‑139 (MQ=255)
gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgc > 1:7170/1‑139 (MQ=255)
cTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg < 1:1013508/139‑1 (MQ=255)
cTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg < 2:216976/139‑1 (MQ=255)
aaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt < 2:7170/139‑1 (MQ=255)
cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt < 1:523318/139‑1 (MQ=255)
tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg > 1:597575/1‑105 (MQ=255)
tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 2:597575/105‑1 (MQ=255)
ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTg > 2:504916/1‑139 (MQ=255)
ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 2:1033928/139‑1 (MQ=255)
ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 1:568937/139‑1 (MQ=255)
tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTccc > 2:1035225/1‑61 (MQ=255)
tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTccc < 1:1035225/61‑1 (MQ=255)
cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc < 1:436060/139‑1 (MQ=255)
ccTGCTTTTTCCCCTCGGTTTTGATTGAGGATTGCACC‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa < 1:746410/139‑1 (MQ=255)
cTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc < 1:504916/139‑1 (MQ=255)
tttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc < 2:1057772/139‑1 (MQ=255)
tCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca > 2:287842/1‑35 (MQ=255)
tCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca < 1:287842/37‑3 (MQ=255)
ggATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa > 2:353652/1‑99 (MQ=255)
ggATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 1:353652/99‑1 (MQ=255)
|
AATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCC > NC_000913/3100895‑3101138
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
