Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,921,515	C→T	T179T (ACG→ACA)	atpB ←	F0 sector of membrane‑bound ATP synthase, subunit a

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,921,515	0	C	T	100.0%	140.6 / NA	44	T179T (ACG→ACA)	atpB	F0 sector of membrane‑bound ATP synthase, subunit a
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/23); total (21/23)

AGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCC  >  NC_000913/3921393‑3921650

aGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGa                                                                                                                         >  1:459107/1‑139 (MQ=255)

    cACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGACTGAACGGCTGCAGTGTCAACTATTTCGTGAAgcc                                                                                                                     >  1:359859/1‑139 (MQ=255)

                        aaCAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGa                                                                                                 <  1:484534/139‑1 (MQ=255)

                        aaCAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGa                                                                                                 <  1:698756/139‑1 (MQ=255)

                         aCAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGAt                                                                                                >  1:164756/1‑139 (MQ=255)

                          cAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATg                                                                                               <  2:459107/139‑1 (MQ=255)

                          cAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATg                                                                                               <  2:359859/139‑1 (MQ=255)

                               gCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTa                                                                                          >  2:759254/1‑139 (MQ=255)

                                    ccGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACa                                                                                     <  2:118479/139‑1 (MQ=255)

                                       aGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGaa                                                                                  >  2:395908/1‑139 (MQ=255)

                                        gTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAAt                                                                                 <  2:113779/139‑1 (MQ=255)

                                                     ggACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACg                                                                    <  1:395908/139‑1 (MQ=255)

                                                       aCAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAAGGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTATCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGcc                                                                  >  2:537535/1‑139 (MQ=255)

                                                       aCAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGcc                                                                  <  2:295424/139‑1 (MQ=255)

                                                       aCAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGcc                                                                  <  1:117786/139‑1 (MQ=255)

                                                         agcagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                                <  2:547188/139‑1 (MQ=255)

                                                         agcagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                                <  1:759254/139‑1 (MQ=255)

                                                         agcagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                                <  1:395367/139‑1 (MQ=255)

                                                           cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGt                                                              >  2:635750/1‑139 (MQ=255)

                                                           cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGt                                                              >  1:507811/1‑139 (MQ=255)

                                                                ttACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                                >  2:780354/1‑132 (MQ=255)

                                                                ttACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                                <  1:780354/132‑1 (MQ=255)

                                                                 tACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATa                                                        >  1:637190/1‑139 (MQ=255)

                                                                   ccccTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATATGCCCAGTGCCATAGa                                                      <  1:537535/139‑1 (MQ=255)

                                                                      cTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGGGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTCATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAg                                                   >  1:750167/1‑139 (MQ=255)

                                                                        tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGaa                                                                                       >  2:758710/1‑101 (MQ=255)

                                                                        tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGaa                                                                                       <  1:758710/101‑1 (MQ=255)

                                                                        tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCg                                                 >  2:345645/1‑139 (MQ=255)

                                                                        tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCg                                                 >  2:301269/1‑139 (MQ=255)

                                                                            ggATTAAGTTGACAGGAATGAACGCCCAGTGATTGAGCGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTAc                                             >  1:692823/1‑139 (MQ=255)

                                                                                 aaGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCa                                        <  1:301269/139‑1 (MQ=255)

                                                                                 aaGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCa                                        >  2:112395/1‑139 (MQ=255)

                                                                                        cAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGc                                 >  2:662923/1‑139 (MQ=255)

                                                                                                     ccAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCa                                        <  1:631254/119‑1 (MQ=255)

                                                                                                     ccAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCa                                        >  2:631254/1‑119 (MQ=255)

                                                                                                      cAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCacac                   <  1:6066/139‑1 (MQ=255)

                                                                                                       aGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACg                  <  1:635750/139‑1 (MQ=255)

                                                                                                       aGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACg                  <  2:640674/139‑1 (MQ=255)

                                                                                                               aaCGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTgcag          >  1:70965/1‑139 (MQ=255)

                                                                                                                   gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAg                                                                              >  1:5670/1‑67 (MQ=255)

                                                                                                                   gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAg                                                                              <  2:5670/67‑1 (MQ=255)

                                                                                                                   gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACcaca                    <  2:187472/125‑1 (MQ=255)

                                                                                                                   gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACcaca                    >  1:187472/1‑125 (MQ=255)

                                                                                                                       cAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGAccc  <  1:662923/139‑1 (MQ=255)

AGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCC  >  NC_000913/3921393‑3921650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A