Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,921,515	C→T	T179T (ACG→ACA)	atpB ←	F0 sector of membrane‑bound ATP synthase, subunit a

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,921,515	0	C	T	100.0%	157.2 / NA	49	T179T (ACG→ACA)	atpB	F0 sector of membrane‑bound ATP synthase, subunit a
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/24); total (25/24)

AGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAG  >  NC_000913/3921381‑3921646

aGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCa                                                                                                                                 >  2:987544/1‑139 (MQ=255)

 gAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCaa                                                                                                                                <  1:987544/139‑1 (MQ=255)

                      cATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGAt                                                                                                           <  2:1120224/139‑1 (MQ=255)

                            tGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCttt                                                                                                     <  1:280126/139‑1 (MQ=255)

                             gTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCaa                                                                                                                                <  2:38022/111‑1 (MQ=255)

                             gTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCaa                                                                                                                                >  1:38022/1‑111 (MQ=255)

                                    aaCAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGa                                                                                             <  1:494784/139‑1 (MQ=255)

                                     aCAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGAt                                                                                            >  2:475294/1‑139 (MQ=255)

                                      cAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATg                                                                                           >  2:343255/1‑139 (MQ=255)

                                                   aGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGaa                                                                              >  2:1017500/1‑139 (MQ=255)

                                                        aaCTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAgg                                                                         <  2:933815/139‑1 (MQ=255)

                                                         aCTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCaa                                                                                                                                <  2:19196/83‑1 (MQ=255)

                                                         aCTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCaa                                                                                                                                >  1:19196/1‑83 (MQ=255)

                                                         aCTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGa                                                                        <  1:343255/139‑1 (MQ=255)

                                                             gTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATaaa                                                                    >  1:163570/1‑139 (MQ=255)

                                                                     agcagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                            <  1:475294/139‑1 (MQ=255)

                                                                     agcagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                            <  1:1230995/139‑1 (MQ=255)

                                                                       cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGa                                                                                             >  2:1455850/1‑104 (MQ=255)

                                                                       cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGa                                                                                             <  1:1455850/104‑1 (MQ=255)

                                                                       cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGt                                                          >  1:456928/1‑139 (MQ=255)

                                                                       cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGt                                                          >  1:274421/1‑139 (MQ=255)

                                                                           cTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTg                                                                                        >  2:1184046/1‑105 (MQ=255)

                                                                           cTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTg                                                                                        <  1:1184046/105‑1 (MQ=255)

                                                                                    tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTg                                                                                        >  1:853493/1‑96 (MQ=255)

                                                                                    tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTg                                                                                        <  2:853493/96‑1 (MQ=255)

                                                                                    tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTg                                                                                        >  2:1074968/1‑96 (MQ=255)

                                                                                    tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTg                                                                                        <  1:1074968/96‑1 (MQ=255)

                                                                                    tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCg                                             >  2:1661678/1‑139 (MQ=255)

                                                                                           ttAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGtt                                      >  1:522365/1‑139 (MQ=255)

                                                                                                   aCAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGccgccg                                                                                                             >  2:991225/1‑60 (MQ=255)

                                                                                                   aCAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGccgccg                                                                                                             <  1:991225/60‑1 (MQ=255)

                                                                                                    cAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGGCAACTCTTTCGTGAAGCCGCCGATGCCTTCCATTTTGATGCTGTAGAACAGAATCCGGATCAATACGCCCAGTGCCATAGACAGCATTCCGTTCACGTCCGc                             >  2:235557/1‑139 (MQ=255)

                                                                                                            aaCGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGaa                     >  2:930641/1‑139 (MQ=255)

                                                                                                             aCGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGaa                                                                                   >  1:101495/1‑76 (MQ=255)

                                                                                                             aCGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGaa                                                                                   <  2:101495/76‑1 (MQ=255)

                                                                                                              cGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATg                                                                                           >  2:264030/1‑67 (MQ=255)

                                                                                                              cGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATg                                                                                           <  1:264030/67‑1 (MQ=255)

                                                                                                              cGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAAcc                   >  1:1645059/1‑139 (MQ=255)

                                                                                                               gCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                            >  1:737675/1‑97 (MQ=255)

                                                                                                               gCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                            <  2:737675/97‑1 (MQ=255)

                                                                                                                 ccAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACcaca                >  2:777255/1‑139 (MQ=255)

                                                                                                                   aGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACg              <  2:522365/139‑1 (MQ=255)

                                                                                                                    gTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGc             <  2:456928/139‑1 (MQ=255)

                                                                                                                        ttGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTg         <  1:777255/139‑1 (MQ=255)

                                                                                                                            aCGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTgcagg     <  1:930641/139‑1 (MQ=255)

                                                                                                                              ggCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTgcaggca   <  2:698606/139‑1 (MQ=255)

                                                                                                                               gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTgcaggcag  >  1:37746/1‑139 (MQ=255)

                                                                                                                                cTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                            >  1:777353/1‑80 (MQ=255)

                                                                                                                                cTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa                                                            <  2:777353/80‑1 (MQ=255)

AGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAG  >  NC_000913/3921381‑3921646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A