Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,820,029 |
A→G |
V381A (GTG→GCG) |
ligB ← |
DNA ligase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,820,029 | 0 | A | G | 100.0%
| 93.0
/ NA
| 27 | V381A (GTG→GCG) | ligB | DNA ligase B |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/12); total (15/12) |
AGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACT > NC_000913/3819953‑3820104
|
aGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGcgccacgcca < 1:684796/80‑1 (MQ=255)
aGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCAcatc < 1:684797/81‑1 (MQ=255)
tCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGaa > 2:684833/1‑81 (MQ=255)
gTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCt > 2:684842/1‑80 (MQ=255)
gTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCt > 1:684825/1‑80 (MQ=255)
gCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCgg > 2:684852/1‑80 (MQ=255)
ggTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGc > 2:684844/1‑79 (MQ=255)
ggTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCaa < 1:684798/81‑1 (MQ=255)
tCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCt < 2:684799/80‑1 (MQ=255)
cTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa > 2:684831/1‑81 (MQ=255)
cTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa > 1:684839/1‑81 (MQ=255)
cTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa > 1:684847/1‑81 (MQ=255)
gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc < 2:684801/79‑1 (MQ=255)
gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc < 2:684802/79‑1 (MQ=255)
gCGGTGTCGGTTTTGTACGTTCTGCACCACGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc < 2:684800/79‑1 (MQ=255)
cGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACgag < 2:684803/81‑1 (MQ=255)
ggTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACgag > 2:684830/1‑80 (MQ=255)
gtgtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGaa > 2:684840/1‑81 (MQ=255)
gtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATc > 2:684836/1‑81 (MQ=255)
gtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATc > 2:684851/1‑81 (MQ=255)
ttttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATc < 1:684804/81‑1 (MQ=255)
tttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACTCTGACCGGCAAGGCTGACGAGAATCTGATCa > 2:684846/1‑81 (MQ=255)
cGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAgg < 2:684805/80‑1 (MQ=255)
gTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcg < 1:684806/81‑1 (MQ=255)
tCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcgc > 1:684837/1‑80 (MQ=255)
cTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCa < 1:684807/80‑1 (MQ=255)
cACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAatat < 1:684808/81‑1 (MQ=255)
cgcgCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc > 1:684845/1‑80 (MQ=255)
gccacgccacATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACt > 2:684866/1‑81 (MQ=255)
|
AGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACT > NC_000913/3819953‑3820104
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
